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Cellosaurus DH82 (CVCL_2018)

[Text version]
Cell line name DH82
Synonyms DH-82; DH 82
Accession CVCL_2018
Resource Identification Initiative To cite this cell line use: DH82 (RRID:CVCL_2018)
Comments Part of: FACC canine tumor cell line panel.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10389.
Characteristics: Can be infected by Trypanosoma cruzi which can then replicate and differentiate inside the cell.
Characteristics: Susceptible to infection by Ehrlichia ruminantium (PubMed=11585095; PubMed=12234001).
Virology: Supports the replication of equine infectious anemia virus (EIAV) (PubMed=11377712).
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 26 hours (PubMed=27257868).
Omics: Deep exome analysis.
Omics: Transcriptome analysis by microarray.
Sequence variations
  • Mutation; VGNC; 45168; PTPN11; Simple; p.Gly503Val (c.1508G>T); Zygosity=Homozygous (PubMed=31175136).
Disease Canine histiocytic sarcoma (NCIt: C128125)
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Golden Retriever.
Hierarchy Children:
CVCL_3461 (DH82ECOK)
Sex of cell Male
Age at sampling 10Y
Category Cancer cell line
STR profile Source(s): PubMed=27197945; PubMed=31175136

Dog FHC2010231
Dog FHC2054156,168
Dog FHC2079275
Dog PEZ1119,123
Dog PEZ12270,285
Dog PEZ20176
Dog PEZ3121
Dog PEZ5107
Dog PEZ6179
Dog PEZ8232,240

Run an STR similarity search on this cell line
Web pages https://web.archive.org/web/20201109215324/www.infarktforschung.de/macrophages_cell_lines.html

PubMed=3350786; DOI=10.1007/BF02623551
Wellman M.L., Krakowka S., Jacobs R.M., Kociba G.J.
A macrophage-monocyte cell line from a dog with malignant histiocytosis.
In Vitro Cell. Dev. Biol. 24:223-229(1988)

Dawson J.E., Rikihisa Y.
Growing Ehrlichia species in a continuous cell line.
Patent number US5192679, 09-Mar-1993

PubMed=11377712; DOI=10.1016/s0166-0934(01)00288-9
Hines R., Maury W.
DH82 cells: a macrophage cell line for the replication and study of equine infectious anemia virus.
J. Virol. Methods 95:47-56(2001)

Zweygarth E., Josemans A.I.
Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line.
Onderstepoort J. Vet. Res. 68:155-157(2001)

Zweygarth E., Josemans A.I., Van Strijp M.F., Van Heerden H., Allsopp M.T.E.P., Allsopp B.A.
The Kumm isolate of Ehrlichia ruminantium: in vitro isolation, propagation and characterization.
Onderstepoort J. Vet. Res. 69:147-153(2002)

PubMed=21908323; DOI=10.1177/1040638711408064
O'Donoghue L.E., Rivest J.P., Duval D.L.
Polymerase chain reaction-based species verification and microsatellite analysis for canine cell line validation.
J. Vet. Diagn. Invest. 23:780-785(2011)

PubMed=25534080; DOI=10.1016/j.vetimm.2014.11.006
Heinrich F., Contioso V.B., Stein V.M., Carlson R., Tipold A., Ulrich R., Puff C., Baumgartner W., Spitzbarth I.
Passage-dependent morphological and phenotypical changes of a canine histiocytic sarcoma cell line (DH82 cells).
Vet. Immunol. Immunopathol. 163:86-92(2015)

PubMed=27257868; DOI=10.1371/journal.pone.0156689
Maeda J., Froning C.E., Brents C.A., Rose B.J., Thamm D.H., Kato T.A.
Intrinsic radiosensitivity and cellular characterization of 27 canine cancer cell lines.
PLoS ONE 11:E0156689-E0156689(2016)

PubMed=26842912; DOI=10.1111/vco.12197
Hartley G., Faulhaber E., Caldwell A., Coy J., Kurihara J., Guth A., Regan D., Dow S.
Immune regulation of canine tumour and macrophage PD-L1 expression.
Vet. Comp. Oncol. 15:534-549(2017)

PubMed=27197945; DOI=10.1111/vco.12192
Fowles J.S., Dailey D.D., Gustafson D.L., Thamm D.H., Duval D.L.
The Flint Animal Cancer Center (FACC) canine tumour cell line panel: a resource for veterinary drug discovery, comparative oncology and translational medicine.
Vet. Comp. Oncol. 15:481-492(2017)

PubMed=28620374; DOI=10.3389/fimmu.2017.00604
Mendonca P.H.B., da Rocha R.F.D.B., de Braz Moraes J.B., LaRocque-de-Freitas I.F., Logullo J., Morrot A., Nunes M.P., Freire-de-Lima C.G., Decote-Ricardo D.
Canine macrophage DH82 cell line as a model to study susceptibility to Trypanosoma cruzi infection.
Front. Immunol. 8:604.1-604.11(2017)

PubMed=31175136; DOI=10.1158/1535-7163.MCT-18-1346
Das S., Idate R., Cronise K.E., Gustafson D.L., Duval D.L.
Identifying candidate druggable targets in canine cancer cell lines using whole exome sequencing.
Mol. Cancer Ther. 18:1460-1471(2019)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cell line collections (Providers) ATCC; CRL-10389
BCRC; 60110
BCRJ; 0077
CLS; 305003
ECACC; 94062922
ICLC; ATL96008
KCB; KCB 2014070YJ
Cell line databases/resources CLO; CLO_0002775
CLDB; cl1058
CLDB; cl1059
CCRID; 1101CAN-PUMC000818
Encyclopedic resources Wikidata; Q54831065
Gene expression databases GEO; GSM1974787
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number23