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Cellosaurus U373-MAGI (CVCL_1F84)

[Text version]
Cell line name U373-MAGI
Synonyms U373-CD4-LTR/beta-gal; U373-Multinuclear Activation of a Galactosidase Indicator
Accession CVCL_1F84
Resource Identification Initiative To cite this cell line use: U373-MAGI (RRID:CVCL_1F84)
Comments Population: Caucasian.
Characteristics: Transfected with a construct pEQ447 containing the HIV-1 LTR fused to a lacZ reporter gene that contains a MPKKKRK N-terminal sequence directing it to the nucleus (PubMed=7690415).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1678; CD4.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E8AN (U373-CD4)
Children:
CVCL_1F85 (U373-MAGI-CCR5)CVCL_1F86 (U373-MAGI-CXCR4)
Sex of cell Male
Age at sampling 75Y
Category Cancer cell line
Publications

PubMed=7690415; DOI=10.1128/JVI.67.10.5939-5947.1993; PMCID=PMC238014
Robert D. Harrington, Adam Philip Geballe;
Cofactor requirement for human immunodeficiency virus type 1 entry into a CD4-expressing human cell line.
J. Virol. 67:5939-5947(1993)

PubMed=9201229; DOI=10.1006/viro.1997.8606
Marie A. Vodicka, Wei Chun Goh, Lily I. Wu, Mark E. Rogel, Steven R. Bartz, Vicki L. Schweickart, Carol J. Raport, Michael Emerman;
Indicator cell lines for detection of primary strains of human and simian immunodeficiency viruses.
Virology 233:193-198(1997)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-3595
Chemistry resources ChEMBL-Cells; CHEMBL3886081
PubChem_Cell_line; CVCL_1F84
Encyclopedic resources Wikidata; Q54973736
Entry history
Entry creation08-Jul-2015
Last entry update14-Aug-2025
Version number15