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Cellosaurus Ras[V12]-H3 (CVCL_1B60)

[Text version]
Cell line name Ras[V12]-H3
Synonyms Ras-H3; Ras H3; R3
Accession CVCL_1B60
Resource Identification Initiative To cite this cell line use: Ras[V12]-H3 (RRID:CVCL_1B60)
Comments Group: Insect cell line.
Doubling time: 45 hours (DGRC=200).
Genetic integration: Method=Transfection/transduction; Gene=FlyBase_Gene; FBgn0003205; Ras85D (Note=With p.Gly12Val).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein).
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Sex of cell Male
Age at sampling Embryonic stage
Category Transformed cell line
Publications

PubMed=18670627; DOI=10.1371/journal.pgen.1000142; PMCID=PMC2474701
Amanda Alice Simcox, Sayan Mitra, Sharon L. Truesdell, Litty Paul, Ting Chen, Jonathan P. Butchar, Steven E. Justiniano;
Efficient genetic method for establishing Drosophila cell lines unlocks the potential to create lines of specific genotypes.
PLoS Genet. 4:e1000142.1-e1000142.12(2008)

PubMed=26438832; DOI=10.1073/pnas.1517729112; PMCID=PMC4620889
Mary-Lee Dequeant, Delphine Fagegaltier, Yan-Hui Hu, Kerstin Spirohn, Amanda Alice Simcox, Gregory James Hannon, Norbert Perrimon;
Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization.
Proc. Natl. Acad. Sci. U.S.A. 112:12974-12979(2015)

PubMed=26772746; DOI=10.1534/g3.115.023366; PMCID=PMC4777130
Marcus Stoiber, Susan E. Celniker, Lucy Cherbas, Ben Brown, Peter Cherbas;
Diverse hormone response networks in 41 independent Drosophila cell lines.
G3 (Bethesda) 6:683-694(2016)

Cross-references
Cell line collections (Providers) DGRC; 200
Kerafast; EF4004
Cell line databases/resources FlyBase_Cell_line; FBtc0000200
Encyclopedic resources Wikidata; Q54949221
Entry history
Entry creation08-Jul-2015
Last entry update10-Apr-2025
Version number16