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Cellosaurus S2R+-Fucci (CVCL_1B58)

[Text version]
Cell line name S2R+-Fucci
Synonyms S2R+-FUCCI; S2R+_FUCCI
Accession CVCL_1B58
Resource Identification Initiative To cite this cell line use: S2R+-Fucci (RRID:CVCL_1B58)
Comments Group: Insect cell line.
Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
Characteristics: Transfected with a Fucci like probe which consist of a nuclear eGFP fused to an E2F1 degron and unlocalized mRFP1 fused to a CycB degron.
Doubling time: ~35 hours (DGRC=243).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: mRFP1, a bright red fluorescent protein derived from drFP583 (DsRed).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R.
Hierarchy Parent: CVCL_Z831 (S2R+)
Sex of cell Male
Age at sampling Late embryonic stage
Category Spontaneously immortalized cell line
Cross-references
Cell line collections (Providers) DGRC; 243
Cell line databases/resources FlyBase_Cell_line; FBtc0000243
Encyclopedic resources Wikidata; Q54951887
Entry history
Entry creation08-Jul-2015
Last entry update02-May-2024
Version number9