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Cellosaurus SK-PN-DW (CVCL_1703)

[Text version]
Cell line name SK-PN-DW
Synonyms SKPNDW
Accession CVCL_1703
Resource Identification Initiative To cite this cell line use: SK-PN-DW (RRID:CVCL_1703)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Population: Caucasian.
Karyotypic information: Has lost chromosome Y as well as one copy each of chromosomes 11, 13, 17 and 18 (PubMed=31046733).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Retroperitoneal space; UBERON=UBERON_0003693.
Sequence variations
  • Gene fusion; HGNC; HGNC:3508; EWSR1 + HGNC; HGNC:3749; FLI1; Name(s)=EWSR1-FLI1, EWS-FLI1; Note=Type 1 fusion (Ex7/Ex6) (PubMed=8040301).
  • Mutation; HGNC; HGNC:9884; RB1; Simple; p.Trp78Ter (c.234G>A); ClinVar=VCV001456015; Zygosity=Homozygous (Cosmic-CLP=909731; DepMap=ACH-001193).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Cys176Phe (c.527G>T); ClinVar=VCV000376569; Zygosity=Homozygous (Cosmic-CLP=909731; DepMap=ACH-001193).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North1.81
East Asian, South0
South Asian0
European, North62.77
European, South35.42
Disease Primitive neuroectodermal tumor (NCIt: C3716)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 17Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2139; Cosmic-CLP=909731; ECACC=94092301

Markers:
AmelogeninX
CSF1PO12
D2S133817,25
D3S135814,16
D5S81813,14
D7S8208,14
D8S117913,15
D13S3179
D16S53912
D18S5116
D19S43312,13
D21S1129,31
FGA19,22
Penta D10,11
Penta E14,16
TH016
TPOX8,9
vWA16

Run an STR similarity search on this cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-crl-2139
Publications

PubMed=2987426; DOI=10.1007/BF00165170
Lawrence Helson, Christiane Helson;
Human neuroblastoma cells and 13-cis-retinoic acid.
J. Neurooncol. 3:39-41(1985)

PubMed=3024811; DOI=10.1016/0165-4608(87)90084-7
Venkateswara R. Potluri, Fred Gilbert, Christine Helsen, Lawrence Helson;
Primitive neuroectodermal tumor cell lines: chromosomal analysis of five cases.
Cancer Genet. Cytogenet. 24:75-86(1987)

DOI=10.1016/B978-0-12-333530-2.50006-X
Mark A. Israel, Carol Jeanne Thiele;
Tumor cell lines of the peripheral nervous system.
(In book chapter) Atlas of human tumor cell lines; Hay, Robert J. & Park, Jae-Gahb & Gazdar, Adi F. (eds.); pp.43-78; Academic Press; New York; USA (1994)

PubMed=8040301; DOI=10.1172/JCI117360; PMCID=PMC295111
Marco Giovannini, Jaclyn A. Biegel, Massimo Serra, Jian-Ying Wang, Ya-Lin H. Wei, Lynn M. Nycum, Beverly S. Emanuel, Glen A. Evans;
EWS-erg and EWS-Fli1 fusion transcripts in Ewing's sarcoma and primitive neuroectodermal tumors with variant translocations.
J. Clin. Invest. 94:489-496(1994)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=22142829; DOI=10.1158/1078-0432.CCR-11-2056; PMCID=PMC3271129
Neerav Shukla, Nabahet Ameur, Ismail Yilmaz, Khedoudja Nafa, Chyau-Yueh Lau, Angela Marchetti, Laetitia Borsu, Frederic G. Barr, Marc Ladanyi;
Oncogene mutation profiling of pediatric solid tumors reveals significant subsets of embryonal rhabdomyosarcoma and neuroblastoma with mutated genes in growth signaling pathways.
Clin. Cancer Res. 18:748-757(2012)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31046733; DOI=10.1186/s12885-019-5625-1; PMCID=PMC6498632
Na Du, Wan-Guo Bao, Kai-Yu Zhang, Xiang-Lan Lu, Rebecca Crew, Xian-Fu Wang, Guang-Ming Liu, Feng Wang;
Cytogenetic characterization of the malignant primitive neuroectodermal SK-PN-DW tumor cell line.
BMC Cancer 19:412.1-412.12(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

DOI=10.5282/edoc.27750
Martin Franz Orth;
Systematic multi-omics profiling of Ewing sarcoma cell lines.
Thesis PhD (2021); Ludwig Maximilians University of Munich; Munich; Germany

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-2139
ECACC; 94092301
Cell line databases/resources CLO; CLO_0009064
CLDB; cl4358
cancercelllines; CVCL_1703
Cell_Model_Passport; SIDM01097
Cosmic-CLP; 909731
DepMap; ACH-001193
LINCS_LDP; LCL-1048
Biological sample resources BioSample; SAMN03472556
BioSample; SAMN10989621
CRISP screens repositories BioGRID_ORCS_Cell_line; 1015
Chemistry resources ChEMBL-Cells; CHEMBL3308886
ChEMBL-Targets; CHEMBL2366108
GDSC; 909731
PharmacoDB; SKPNDW_1412_2019
PubChem_Cell_line; CVCL_1703
Encyclopedic resources Wikidata; Q54954519
Gene expression databases ArrayExpress; E-MTAB-3610
FANTOM5_SSTAR; 10719-109I8
GEO; GSM1670456
Polymorphism and mutation databases Cosmic; 684073
Cosmic; 909731
Cosmic; 1078390
Cosmic; 1718431
IARC_TP53; 27245
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number38