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Cellosaurus RPMI-2650 (CVCL_1664)

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Cell line name RPMI-2650
Synonyms RPMI 2650; RPMI2650; R.P.M.I. #2650; Roswell Park Memorial Institute 2650
Accession CVCL_1664
Resource Identification Initiative To cite this cell line use: RPMI-2650 (RRID:CVCL_1664)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: KuDOS 95 cell line panel.
Population: Caucasian.
Doubling time: ~40-50 hours (DSMZ=ACC-287).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Misspelling: CCL30; Note=Based on the ATCC catalog number.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; 13575; BRD4 + HGNC; 29919; NUTM1; Name(s)=BRD4-NUTM1, BRD4-NUT; Note=BRD4 exon 15 fused to NUTM1 exon 2 (PubMed=25485619; PubMed=29348827).
HLA typing Source: PubMed=77569
Class I

Source: PubMed=26589293
Class I
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0.9
East Asian, North1.71
East Asian, South0
South Asian0
European, North68.78
European, South28.23
Disease Head and neck squamous cell carcinoma (NCIt: C34447)
NUT carcinoma (NCIt: C45716)
NUT midline carcinoma (ORDO: Orphanet_443167)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 52Y
Category Cancer cell line
STR profile Source(s): ATCC=CCL-30; CLS=300323; Cosmic-CLP=909700; DepMap=ACH-001385; DSMZ=ACC-287; ECACC=88031602; JCRB=JCRB9058; KCLB=10030; PubMed=25877200

Penta D9,10
Penta E11,19

Run an STR similarity search on this cell line

Moore G.E., Mount D., Tara G., Schwartz N.
Growth of human tumor cells in suspension cultures.
Cancer Res. 23:1735-1741(1963)

PubMed=5831238; DOI=10.1016/0014-4827(65)90022-4
Moorhead P.S.
Human tumor cell line with a quasi-diploid karyotype (RPMI 2650).
Exp. Cell Res. 39:190-196(1965)

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=174085; DOI=10.1073/pnas.72.12.4971
Stiles C.D., Desmond W. Jr., Sato G.H., Saier M.H. Jr.
Failure of human cells transformed by simian virus 40 to form tumors in athymic nude mice.
Proc. Natl. Acad. Sci. U.S.A. 72:4971-4975(1975)

Stiles C.D., Desmond W. Jr., Chuman L.M., Sato G.H., Saier M.H. Jr.
Growth control of heterologous tissue culture cells in the congenitally athymic nude mouse.
Cancer Res. 36:1353-1360(1976)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=77569; DOI=10.1111/j.1399-0039.1978.tb01259.x
Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.
Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.
Tissue Antigens 11:279-286(1978)

Frampton R.J., Suva L.J., Eisman J.A., Findlay D.M., Moore G.E., Moseley J.M., Martin T.J.
Presence of 1,25-dihydroxyvitamin D3 receptors in established human cancer cell lines in culture.
Cancer Res. 42:1116-1119(1982)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

Carey T.E.
Head and neck tumor cell lines.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.79-120; Academic Press; New York (1994)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=29348827; DOI=10.18632/oncotarget.22862
Stirnweiss A., Oommen J., Kotecha R.S., Kees U.R., Beesley A.H.
Molecular-genetic profiling and high-throughput in vitro drug screening in NUT midline carcinoma-an aggressive and fatal disease.
Oncotarget 8:112313-112329(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

Cell line collections (Providers) ATCC; CCL-30
BCRC; 60296
CLS; 300323
ECACC; 88031602
KCLB; 10030
Cell line databases/resources CLO; CLO_0008869
CLO; CLO_0008876
CLDB; cl4174
CLDB; cl4175
CLDB; cl4176
cancercelllines; CVCL_1664
Cell_Model_Passport; SIDM00809
Cosmic-CLP; 909700
DepMap; ACH-001385
DSMZCellDive; ACC-287
IGRhCellID; RPMI2650%20GSE9585
Anatomy/cell type resources BTO; BTO:0004205
Biological sample resources BioSample; SAMN01821660
BioSample; SAMN01821717
BioSample; SAMN03473151
Chemistry resources ChEMBL-Cells; CHEMBL3308780
ChEMBL-Targets; CHEMBL1075566
GDSC; 909700
PharmacoDB; RPMI2650_1324_2019
PubChem_Cell_line; CVCL_1664
Encyclopedic resources Wikidata; Q54951175
Experimental variables resources EFO; EFO_0006478
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-3610
GEO; GSM960607
GEO; GSM960608
GEO; GSM960609
GEO; GSM960610
GEO; GSM960611
GEO; GSM960612
GEO; GSM960613
GEO; GSM1374853
GEO; GSM1374854
GEO; GSM1670386
Polymorphism and mutation databases Cosmic; 686978
Cosmic; 909700
Cosmic; 1006465
Cosmic; 1017789
Cosmic; 1041670
Cosmic; 1339925
Cosmic; 2043886
IARC_TP53; 21290
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number42