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Cellosaurus EW-8 (CVCL_1658)

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Cell line name EW-8
Synonyms EW8; EW8(Rh1); RH1; Rh1; RH-1; Rh-1; SJRH-1; SJRH1
Accession CVCL_1658
Secondary accession CVCL_5915; CVCL_V618
Resource Identification Initiative To cite this cell line use: EW-8 (RRID:CVCL_1658)
Comments Problematic cell line: Misclassified. Originally thought to be a rhabdomyosarcoma cell line but shown to be from an Ewing sarcoma (PubMed=17154184).
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: Ewing Sarcoma Cell Line Atlas (ESCLA).
Population: Caucasian.
Doubling time: 30 hours (PubMed=25984343); ~30-40 hours (DSMZ=ACC-493).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; ChIP-seq; FLI1.
Omics: Genomics; ChIP-seq; H3K27ac.
Omics: Genomics; ChIP-seq; H3K27me3.
Omics: Genomics; ChIP-seq; H3K4me3.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Genomics; Whole genome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Phenotyping; shRNA library screening.
Omics: Proteomics.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Bone marrow; UBERON=UBERON_0002371.
Sequence variations
  • Gene fusion; HGNC; HGNC:3508; EWSR1 + HGNC; HGNC:3749; FLI1; Name(s)=EWSR1-FLI1, EWS-FLI1; Note=Type 1 fusion (Ex7/Ex6) (PubMed=17154184; PubMed=25010205; PubMed=36476851).
  • Mutation; HGNC; HGNC:6342; KIT; Simple; p.Arg55Trp (c.163A>T); Zygosity=Heterozygous (Cosmic-CLP=971773; DepMap=ACH-000499).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (PubMed=25010205; Cosmic-CLP=971773; DepMap=ACH-000499).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North1.08
East Asian, South0
South Asian0
European, North66.53
European, South32.38
Disease Ewing sarcoma (NCIt: C4817)
Ewing sarcoma (ORDO: Orphanet_319)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=971773; DSMZ=ACC-493; PubMed=25010205

Markers:
AmelogeninX,Y
CSF1PO11
D2S133816,19
D3S135811,18 (PubMed=25010205)
18 (DSMZ=ACC-493)
D5S81811
D7S8208,12
D8S117914,16
D13S31710
D16S53913
D18S5115
D19S43313,15.2 (PubMed=25010205)
13,16 (DSMZ=ACC-493)
D21S1129,30
FGA22,23
Penta D9,13
Penta E5,16
TH019.3
TPOX8
vWA17

Run an STR similarity search on this cell line
Web pages Info; ESCLA; -; https://hgserver1.amc.nl/cgi-bin/r2/main.cgi?option=about_dscope
Publications

PubMed=8275086; DOI=10.1038/ng1193-230
Galili N., Davis R.J., Fredericks W.J., Mukhopadhyay S., Rauscher F.J. 3rd, Emanuel B.S., Rovera G., Barr F.G.
Fusion of a fork head domain gene to PAX3 in the solid tumour alveolar rhabdomyosarcoma.
Nat. Genet. 5:230-235(1993)

PubMed=7536457; DOI=10.1002/gcc.2870120305
Biegel J.A., Nycum L.M., Valentine V.A., Barr F.G., Shapiro D.N.
Detection of the t(2;13)(q35;q14) and PAX3-FKHR fusion in alveolar rhabdomyosarcoma by fluorescence in situ hybridization.
Genes Chromosomes Cancer 12:186-192(1995)

PubMed=11051265
Petak I., Douglas L., Tillman D.M., Vernes R., Houghton J.A.
Pediatric rhabdomyosarcoma cell lines are resistant to Fas-induced apoptosis and highly sensitive to TRAIL-induced apoptosis.
Clin. Cancer Res. 6:4119-4127(2000)

PubMed=17154184; DOI=10.1002/pbc.21099
Smith M.A., Morton C.L., Phelps D.A., Girtman K., Neale G., Houghton P.J.
SK-NEP-1 and Rh1 are Ewing family tumor lines.
Pediatr. Blood Cancer 50:703-706(2008)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=23578105; DOI=10.1111/cas.12173; PMCID=PMC7657110
Nishimura R., Takita J., Sato-Otsubo A., Kato M., Koh K., Hanada R., Tanaka Y., Kato K., Maeda D., Fukayama M., Sanada M., Hayashi Y., Ogawa S.
Characterization of genetic lesions in rhabdomyosarcoma using a high-density single nucleotide polymorphism array.
Cancer Sci. 104:856-864(2013)

PubMed=23882450; DOI=10.3389/fonc.2013.00183; PMCID=PMC3713458
Hinson A.R.P., Jones R., Crose L.E.S., Belyea B.C., Barr F.G., Linardic C.M.
Human rhabdomyosarcoma cell lines for rhabdomyosarcoma research: utility and pitfalls.
Front. Oncol. 3:183.1-183.12(2013)

PubMed=25010205; DOI=10.1371/journal.pgen.1004475; PMCID=PMC4091782
Brohl A.S., Solomon D.A., Chang W., Wang J.-J., Song Y., Sindiri S., Patidar R., Hurd L., Chen L., Shern J.F., Liao H.-L., Wen X.-Y., Gerard J., Kim J.-S., Lopez Guerrero J.A., Machado I., Wai D.H., Picci P., Triche T.J., Horvai A.E., Miettinen M.M., Wei J.S., Catchpoole D., Llombart-Bosch A., Waldman T.A., Khan J.
The genomic landscape of the Ewing sarcoma family of tumors reveals recurrent STAG2 mutation.
PLoS Genet. 10:e1004475.1-e1004475.13(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035.1-140035.12(2014)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074; PMCID=PMC4636476
Teicher B.A., Polley E.C., Kunkel M.W., Evans D., Silvers T.E., Delosh R.M., Laudeman J., Ogle C., Reinhart R., Selby M., Connelly J., Harris E., Monks A., Morris J.
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

DOI=10.5282/edoc.27750
Orth M.F.
Systematic multi-omics profiling of Ewing sarcoma cell lines.
Thesis PhD (2021); Ludwig Maximilians University of Munich; Munich; Germany

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

PubMed=36476851; DOI=10.1016/j.celrep.2022.111761; PMCID=PMC10333306
Orth M.F., Surdez D., Faehling T., Ehlers A.C., Marchetto A., Grossetete-Lalami S., Volckmann R., Zwijnenburg D.A., Gerke J.S., Zaidi S., Alonso J., Sastre A., Baulande S., Sill M., Cidre-Aranaz F., Ohmura S., Kirchner T., Hauck S.M., Reischl E., Gymrek M., Pfister S.M., Strauch K., Koster J., Delattre O., Grunewald T.G.P.
Systematic multi-omics cell line profiling uncovers principles of Ewing sarcoma fusion oncogene-mediated gene regulation.
Cell Rep. 41:111761.1-111761.28(2022)

Cross-references
Cell line collections (Providers) DSMZ; ACC-493
Cell line databases/resources CLO; CLO_0009878
cancercelllines; CVCL_1658
Cell_Model_Passport; SIDM00427
Cell_Model_Passport; SIDM01500
Cosmic-CLP; 971773
DepMap; ACH-000499
DepMap; ACH-002194 - Discontinued
DSMZCellDive; ACC-493
LINCS_LDP; LCL-1456
Anatomy/cell type resources BTO; BTO_0005378
Biological sample resources BioSample; SAMN03473566
BioSample; SAMN10988420
CRISP screens repositories BioGRID_ORCS_Cell_line; 172
Chemistry resources ChEMBL-Cells; CHEMBL3308524
ChEMBL-Targets; CHEMBL2366078
GDSC; 971773
PharmacoDB; EW8_361_2019
PharmacoDB; RH1_1310_2019
PubChem_Cell_line; CVCL_1658
Encyclopedic resources Wikidata; Q54833004
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM1670376
GEO; GSM1676298
GEO; GSM1701633
GEO; GSM5359688
GEO; GSM5359689
GEO; GSM5359690
GEO; GSM5359691
GEO; GSM5359692
GEO; GSM5359693
GEO; GSM5362948
GEO; GSM5362949
GEO; GSM5362950
GEO; GSM5362951
GEO; GSM5362952
GEO; GSM5362953
GEO; GSM5363996
GEO; GSM5363997
GEO; GSM5363998
GEO; GSM5363999
GEO; GSM5364000
Polymorphism and mutation databases Cosmic; 801755
Cosmic; 971773
Cosmic; 1037294
Cosmic; 1048107
Cosmic; 1097750
Cosmic; 1104556
Cosmic; 1108744
Cosmic; 2228229
Cosmic; 2294583
IARC_TP53; 27229
Progenetix; CVCL_V618
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number44