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Cellosaurus NCI-H660 (CVCL_1576)

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Cell line name NCI-H660
Synonyms H660; H-660; NCIH660; NCI660
Accession CVCL_1576
Resource Identification Initiative To cite this cell line use: NCI-H660 (RRID:CVCL_1576)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 100 hours (PubMed=25984343).
Microsatellite instability: Stable (MSS) (PubMed=23671654; Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Phenotyping; shRNA library screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; miRNA profiling; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Caution: Was originally published as originating from a small cell lung carcinoma (PubMed=2985257), but was later shown to originate from a prostate small cell carcinoma (PubMed=2569043).
Misspelling: NCI_H600; Cosmic=2669168; Note=Prior to 2020.
Derived from site: Metastatic; Cervical lymph node; UBERON=UBERON_0002429.
Sequence variations
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,68:01
HLA-BB*44:02,57:01
HLA-CC*07:04,07:04
Genome ancestry Source: PubMed=30894373

Origin% genome
African2.18
Native American0
East Asian, North2.77
East Asian, South0
South Asian0
European, North58.05
European, South37
Disease Prostate small cell carcinoma (NCIt: C6766)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 63Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-5813; Cosmic-CLP=1330975; PubMed=11304728; PubMed=14518029; PubMed=25877200

Markers:
AmelogeninX
CSF1PO12
D2S133818,24
D3S135814,18
D5S81811
D7S8208,10
D8S11799,10
D13S3178
D16S53911
D18S5115,16
D19S43315
D21S1128,31
FGA23,24
Penta D9,12
Penta E12
TH017,9.3
TPOX8
vWA17

Run an STR similarity search on this cell line
Publications

PubMed=2985257
Desmond Niall Carney, Adi F. Gazdar, Gerold Bepler, John G. Guccion, Paul J. Marangos, Terry W. Moody, Mark H. Zweig, John D. Minna;
Establishment and identification of small cell lung cancer cell lines having classic and variant features.
Cancer Res. 45:2913-2923(1985)

PubMed=2569043; DOI=10.1093/jnci/81.16.1223
Bruce E. Johnson, Jacqueline Whang-Peng, Susan L. Naylor, Berton Zbar, Hiltrud Brauch, Elaine C. Lee, Alfreda M. Simmons, Edward Russell, Myong H. Nam, Adi F. Gazdar;
Retention of chromosome 3 in extrapulmonary small cell cancer shown by molecular and cytogenetic studies.
J. Natl. Cancer Inst. 81:1223-1228(1989)

PubMed=1563005
Giuseppe Giaccone, James Battey, Adi F. Gazdar, Herbert Kazuto Oie, Muriel Draoui, Terry W. Moody;
Neuromedin B is present in lung cancer cell lines.
Cancer Res. 52:2732s-2736s(1992)

PubMed=8806092; DOI=10.1002/jcb.240630505
Ruby M. Phelps, Bruce E. Johnson, Daniel Carlyle Ihde, Adi F. Gazdar, David P. Carbone, Patrick R. McClintock, Ritva Ilona Linnoila, Mary J. Matthews, Paul A. Bunn Jr., Desmond Niall Carney, John D. Minna, James L. Mulshine;
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. Suppl. 24:32-91(1996)

PubMed=8806103; DOI=10.1002/jcb.240630516
Bruce E. Johnson, Edward Russell, Alfreda M. Simmons, Ruby M. Phelps, Seth M. Steinberg, Daniel Carlyle Ihde, Adi F. Gazdar;
MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.
J. Cell. Biochem. Suppl. 24:210-217(1996)

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Sanjay Modi, Akihito Kubo, Herbert Kazuto Oie, Amy B. Coxon, Ahad Rehmatulla, Frederic J. Kaye;
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=11304728; DOI=10.1002/pros.1045
Adrie van Bokhoven, Marileila Varella-Garcia, Christopher T. Korch, Daphne Hessels, Gary J. Miller;
Widely used prostate carcinoma cell lines share common origins.
Prostate 47:36-51(2001)

PubMed=14518029; DOI=10.1002/pros.10290
Adrie van Bokhoven, Marileila Varella-Garcia, Christopher T. Korch, Widya U. Johannes, E. Erin Smith, Heidi L. Miller, Steven K. Nordeen, Gary J. Miller, Marshall Scott Lucia;
Molecular characterization of human prostate carcinoma cell lines.
Prostate 57:205-225(2003)

CLPUB00698
Adrie van Bokhoven;
Models for prostate cancer. Molecular characterization and critical appraisal of human prostate carcinoma cell lines.
Thesis PhD (2004); Katholieke Universiteit Nijmegen; Nijmegen; Netherlands

PubMed=15643172; DOI=10.1097/01.ju.0000141580.30910.57
Richard E. Sobel, Marianne D. Sadar;
Cell lines used in prostate cancer research: a compendium of old and new lines -- part 1.
J. Urol. 173:342-359(2005)

PubMed=17401460; DOI=10.1593/neo.07103; PMCID=PMC1838578
Kirsten D. Mertz, Sunita R. Setlur, Saravana M. Dhanasekaran, Francesca Demichelis, Sven Perner, Scott Tomlins, Joelle Tchinda, Bharathi Laxman, Robert Louis Vessella, Rameen Beroukhim, Charles Lee ...Show all 13 authors... , Arul M. Chinnaiyan, Mark A. Rubin; Show fewer authors
Molecular characterization of TMPRSS2-ERG gene fusion in the NCI-H660 prostate cancer cell line: a new perspective for an old model.
Neoplasia 9:200-206(2007)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura Eleanor MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23671654; DOI=10.1371/journal.pone.0063056; PMCID=PMC3646030
Yu-Heng Lu, T. David Soong, Olivier Elemento;
A novel approach for characterizing microsatellite instability in cancer cells.
PLoS ONE 8:e63056.1-e63056.10(2013)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
Glenn S. Cowley, Barbara A. Weir, Francisca Vazquez, Pablo Tamayo, Justine A. Scott, Scott Rusin, Alexandra East-Seletsky, Levi D. Ali, William F.J. Gerath, Sarah E. Pantel, Patrick Hall Lizotte ...Show all 40 authors... , Guo-Zhi Jiang, Jessica Hsiao, Aviad Tsherniak, Elizabeth Dwinell, Simon Aoyama, Michael Okamoto, William Harrington, Ellen T. Gelfand, Thomas M. Green, Mark J. Tomko, Shuba Gopal, Terence C. Wong, Hu-Bo Li, Sara Howell, Nicolas Stransky, Ted Liefeld, Dongkeun Jang, Jonathan Bistline, Barbara Hill Meyers, Scott Allen Armstrong, Kenneth Carl Anderson, Kimberly Stegmaier, Michael Reich, David Pellman, Jesse S. Boehm, Jill P. Mesirov, Todd Robert Golub, David E. Root, William Chun Hahn; Show fewer authors
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035.1-140035.12(2014)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27247353; DOI=10.1093/jnci/djw122; PMCID=PMC6279282
Eric C. Polley, Mark W. Kunkel, David Evans, Thomas E. Silvers, Rene M. Delosh, Julie Laudeman, Chad Ogle, Russell Reinhart, Michael Selby, John Connelly, Erik Harris ...Show all 18 authors... , Nicole Fer, Dmitriy Sonkin, Gurmeet Kaur, Anne Monks, Shakun Malik, Joel Morris, Beverly A. Teicher; Show fewer authors
Small cell lung cancer screen of oncology drugs, investigational agents, and gene and microRNA expression.
J. Natl. Cancer Inst. 108:djw122.1-djw122.11(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28766886; DOI=10.1002/cam4.1131; PMCID=PMC5548882
Beverly A. Teicher, Thomas E. Silvers, Michael Selby, Rene M. Delosh, Julie Laudeman, Chad Ogle, Russell Reinhart, Ralph E. Parchment, Julia Krushkal, Dmitriy Sonkin, Lawrence Rubinstein ...Show all 13 authors... , Joel Morris, David Evans; Show fewer authors
Small cell lung carcinoma cell line screen of etoposide/carboplatin plus a third agent.
Cancer Med. 6:1952-1964(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=32586373; DOI=10.1186/s13148-020-00876-8; PMCID=PMC7318526
Julia Krushkal, Thomas E. Silvers, William C. Reinhold, Dmitriy Sonkin, Suleyman Vural, John Connelly, Sudhir Varma, Paul S. Meltzer, Mark W. Kunkel, Annamaria Rapisarda, David Evans ...Show all 13 authors... , Yves Pommier, Beverly A. Teicher; Show fewer authors
Epigenome-wide DNA methylation analysis of small cell lung cancer cell lines suggests potential chemotherapy targets.
Clin. Epigenetics 12:93.1-93.28(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-5813
Cell line databases/resources CLO; CLO_0008102
cancercelllines; CVCL_1576
Cell_Model_Passport; SIDM01123
Cosmic-CLP; 1330975
DepMap; ACH-000177
IGRhCellID; NCIH660
Anatomy/cell type resources BTO; BTO_0001970
Biological sample resources BioSample; SAMN03473052
BioSample; SAMN10988121
Chemistry resources PharmacoDB; NCIH660_1126_2019
Encyclopedic resources Wikidata; Q54908097
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM482672
GEO; GSM648822
GEO; GSM887440
GEO; GSM888520
GEO; GSM1670256
GEO; GSM1887911
GEO; GSM1887993
Polymorphism and mutation databases Cosmic; 877360
Cosmic; 1021869
Cosmic; 1043331
Cosmic; 1075272
Cosmic; 1689713
Cosmic; 2651769
Cosmic; 2669168
IARC_TP53; 21084
IARC_TP53; 21725
LiGeA; CCLE_659
Progenetix; CVCL_1576
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number37