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Cellosaurus NCI-H510A (CVCL_1565)

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Cell line name NCI-H510A
Synonyms H510A; H-510A; NCI-H510; NCIH510; H510; H-510
Accession CVCL_1565
Secondary accession CVCL_3973
Resource Identification Initiative To cite this cell line use: NCI-H510A (RRID:CVCL_1565)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 66 hours (PubMed=2985258); ~1 week (DSMZ=ACC-568).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; miRNA profiling; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Adrenal gland; UBERON=UBERON_0002369.
Sequence variations
  • Mutation; HGNC; HGNC:9884; RB1; Simple; p.Lys265Ter (c.793A>T); Zygosity=Homozygous (ATCC=HTB-184; Cosmic-CLP=753605; DepMap=ACH-000871).
  • Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Gly30Ter (c.88G>T); Zygosity=Homozygous (ATCC=HTB-184; Cosmic-CLP=753605; DepMap=ACH-000871).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg282Gly (c.844C>G); ClinVar=VCV000140821; Zygosity=Homozygous (PubMed=1312696; PubMed=1565469; PubMed=20557307; ATCC=HTB-184; Cosmic-CLP=753605; DepMap=ACH-000871).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,01:01
HLA-BB*07:02,58:05
HLA-CC*07:01,07:01
Class II
HLA-DQDQA1*03:02,05:02
HLA-DRDRB1*04:05,04:05
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.05
Native American0.3
East Asian, North2.01
East Asian, South0
South Asian0
European, North64.53
European, South33.11
Disease Lung small cell carcinoma (NCIt: C4917)
Small cell lung cancer (ORDO: Orphanet_70573)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 53Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-184; Cosmic-CLP=753605; DSMZ=ACC-568; PubMed=25877200

Markers:
AmelogeninX
CSF1PO10,12
D2S133817,24
D3S135816,18
D5S8189,12
D7S82010,11
D8S117915
D13S31711,12
D16S53913
D18S5115,17
D19S43314,15
D21S1130
FGA22
Penta D9,11
Penta E12,16
TH017,9.3
TPOX8
vWA15

Run an STR similarity search on this cell line
Publications

PubMed=2985257
Desmond Niall Carney, Adi F. Gazdar, Gerold Bepler, John G. Guccion, Paul J. Marangos, Terry W. Moody, Mark H. Zweig, John D. Minna;
Establishment and identification of small cell lung cancer cell lines having classic and variant features.
Cancer Res. 45:2913-2923(1985)

PubMed=2985258
Adi F. Gazdar, Desmond Niall Carney, Marion M. Nau, John D. Minna;
Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties.
Cancer Res. 45:2924-2930(1985)

PubMed=2569043; DOI=10.1093/jnci/81.16.1223
Bruce E. Johnson, Jacqueline Whang-Peng, Susan L. Naylor, Berton Zbar, Hiltrud Brauch, Elaine C. Lee, Alfreda M. Simmons, Edward Russell, Myong H. Nam, Adi F. Gazdar;
Retention of chromosome 3 in extrapulmonary small cell cancer shown by molecular and cytogenetic studies.
J. Natl. Cancer Inst. 81:1223-1228(1989)

PubMed=1312696
Domenico D'Amico, David P. Carbone, Tetsuya Mitsudomi, Marion M. Nau, Joseph Fedorko, Edward Russell, Britt Johnson, Dotothy L. Buchhagen, Sara M. Bodner, Ruby M. Phelps, Adi F. Gazdar, John D. Minna;
High frequency of somatically acquired p53 mutations in small-cell lung cancer cell lines and tumors.
Oncogene 7:339-346(1992)

PubMed=1563005
Giuseppe Giaccone, James Battey, Adi F. Gazdar, Herbert Kazuto Oie, Muriel Draoui, Terry W. Moody;
Neuromedin B is present in lung cancer cell lines.
Cancer Res. 52:2732s-2736s(1992)

PubMed=1565469
Sara M. Bodner, John D. Minna, Sandra M. Jensen, Domenico D'Amico, David P. Carbone, Tetsuya Mitsudomi, Joseph Fedorko, Dotothy L. Buchhagen, Marion M. Nau, Adi F. Gazdar, Ritva Ilona Linnoila;
Expression of mutant p53 proteins in lung cancer correlates with the class of p53 gene mutation.
Oncogene 7:743-749(1992)

PubMed=8626706; DOI=10.1074/jbc.271.19.11477
Kathryn A. Quinn, Anthony M. Treston, Edward J. Unsworth, Mae-Jean Miller, Michele Vos, Chris Grimley, James Battey, James L. Mulshine, Frank Cuttitta;
Insulin-like growth factor expression in human cancer cell lines.
J. Biol. Chem. 271:11477-11483(1996)

PubMed=8806092; DOI=10.1002/jcb.240630505
Ruby M. Phelps, Bruce E. Johnson, Daniel C. Ihde, Adi F. Gazdar, David P. Carbone, Patrick R. McClintock, Ritva Ilona Linnoila, Mary J. Matthews, Paul A. Bunn Jr., Desmond Niall Carney, John D. Minna, James L. Mulshine;
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. Suppl. 24:32-91(1996)

PubMed=8806103; DOI=10.1002/jcb.240630516
Bruce E. Johnson, Edward Russell, Alfreda M. Simmons, Ruby M. Phelps, Seth M. Steinberg, Daniel C. Ihde, Adi F. Gazdar;
MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.
J. Cell. Biochem. Suppl. 24:210-217(1996)

PubMed=10037197
Xao X. Tang, Garrett M. Brodeur, Barbara G. Campling, Naohiko Ikegaki;
Coexpression of transcripts encoding EPHB receptor protein tyrosine kinases and their ephrin-B ligands in human small cell lung carcinoma.
Clin. Cancer Res. 5:455-460(1999)

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Sanjay Modi, Akihito Kubo, Herbert Kazuto Oie, Amy B. Coxon, Ahad Rehmatulla, Frederic J. Kaye;
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=17426248; DOI=10.1158/1541-7786.MCR-06-0367
Edward T. Olejniczak, Charles Van Sant, Mark G. Anderson, Gang Wang, Stephen K. Tahir, Guido Sauter, Rick Lesniewski, Dimitri Semizarov;
Integrative genomic analysis of small-cell lung carcinoma reveals correlates of sensitivity to bcl-2 antagonists and uncovers novel chromosomal gains.
Mol. Cancer Res. 5:331-339(2007)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20557307; DOI=10.1111/j.1349-7006.2010.01622.x; PMCID=PMC11158680
Reika Iwakawa, Takashi Kohno, Masatoshi Enari, Tohru Kiyono, Jun Yokota;
Prevalence of human papillomavirus 16/18/33 infection and p53 mutation in lung adenocarcinoma.
Cancer Sci. 101:1891-1896(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22961666; DOI=10.1158/2159-8290.CD-12-0112; PMCID=PMC3567922
Lauren Averett Byers, Jing Wang, Monique B. Nilsson, Junya Fujimoto, Pierre Saintigny, John Stuart Yordy, Uma Giri, Michael Peyton, You-Hong Fan, Li-Xia Diao, Fatemeh Masrorpour ...Show all 28 authors... , Li Shen, Wen-Bin Liu, Boris Duchemann, Praveen Tumula, Vikas Bhardwaj, James Welsh, Stephanie Weber, Bonnie S. Glisson, Neda Kalhor, Ignacio Ivan Wistuba, Luc Girard, Scott M. Lippman, Gordon B. Mills, Kevin Robert Coombes, John N. Weinstein, John D. Minna, John Victor Heymach; Show fewer authors
Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.
Cancer Discov. 2:798-811(2012)

PubMed=23716474; DOI=10.1002/gcc.22076; PMCID=PMC3806277
Reika Iwakawa, Masataka Takenaka, Takashi Kohno, Yoko Shimada, Yasushi Totoki, Tatsuhiro Shibata, Koji Tsuta, Ryo Nishikawa, Masayuki Noguchi, Aiko Sato-Otsubo, Seishi Ogawa, Jun Yokota;
Genome-wide identification of genes with amplification and/or fusion in small cell lung cancer.
Genes Chromosomes Cancer 52:802-816(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27247353; DOI=10.1093/jnci/djw122; PMCID=PMC6279282
Eric C. Polley, Mark W. Kunkel, David Evans, Thomas E. Silvers, Rene M. Delosh, Julie Laudeman, Chad Ogle, Russell Reinhart, Michael Selby, John Connelly, Erik Harris ...Show all 18 authors... , Nicole Fer, Dmitriy Sonkin, Gurmeet Kaur, Anne Monks, Shakun Malik, Joel Morris, Beverly A. Teicher; Show fewer authors
Small cell lung cancer screen of oncology drugs, investigational agents, and gene and microRNA expression.
J. Natl. Cancer Inst. 108:djw122.1-djw122.11(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28766886; DOI=10.1002/cam4.1131; PMCID=PMC5548882
Beverly A. Teicher, Thomas E. Silvers, Michael Selby, Rene M. Delosh, Julie Laudeman, Chad Ogle, Russell Reinhart, Ralph E. Parchment, Julia Krushkal, Dmitriy Sonkin, Lawrence Rubinstein ...Show all 13 authors... , Joel Morris, David Evans; Show fewer authors
Small cell lung carcinoma cell line screen of etoposide/carboplatin plus a third agent.
Cancer Med. 6:1952-1964(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31803961; DOI=10.1002/jcb.29564; PMCID=PMC7496084
James L. Mulshine, Peter Ujhazy, Melissa Antman, Christine M. Burgess, Igor A. Kuzmin, Paul A. Bunn Jr., Bruce E. Johnson, Jack Alan Roth, Harvey I. Pass, Sheila M. Ross, Carolyn R. Aldige ...Show all 13 authors... , Ignacio Ivan Wistuba, John D. Minna; Show fewer authors
From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.
J. Cell. Biochem. 121:3986-3999(2020)

PubMed=32586373; DOI=10.1186/s13148-020-00876-8; PMCID=PMC7318526
Julia Krushkal, Thomas E. Silvers, William C. Reinhold, Dmitriy Sonkin, Suleyman Vural, John Connelly, Sudhir Varma, Paul S. Meltzer, Mark W. Kunkel, Annamaria Rapisarda, David Evans ...Show all 13 authors... , Yves Pommier, Beverly A. Teicher; Show fewer authors
Epigenome-wide DNA methylation analysis of small cell lung cancer cell lines suggests potential chemotherapy targets.
Clin. Epigenetics 12:93.1-93.28(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

PubMed=39154123; DOI=10.1038/s41388-024-03125-x; PMCID=PMC11405284
Aiden Nguyen, Clarissa G. Nunez, Tram Anh Tran, Luc Girard, Michael Peyton, Rodrigo Catalan, Cristina D. Guerena, Kimberley Avila, Benjamin J. Drapkin, Raghav Chandra, John D. Minna, Elisabeth D. Martinez;
Jumonji histone demethylases are therapeutic targets in small cell lung cancer.
Oncogene 43:2885-2899(2024)

Cross-references
Cell line collections (Providers) ATCC; HTB-184
DSMZ; ACC-568
ECACC; 96020944
Cell line databases/resources CLO; CLO_0008092
cancercelllines; CVCL_1565
Cell_Model_Passport; SIDM00927
Cosmic-CLP; 753605
DepMap; ACH-000871
DSMZCellDive; ACC-568
LINCS_LDP; LCL-1856
Anatomy/cell type resources BTO; BTO_0003023
Biological sample resources BioSample; SAMN03473048
BioSample; SAMN10987639
Chemistry resources ChEMBL-Cells; CHEMBL3308322
ChEMBL-Targets; CHEMBL2366332
GDSC; 753605
PharmacoDB; NCIH510A_1116_2019
PubChem_Cell_line; CVCL_1565
Encyclopedic resources Wikidata; Q54908071
Experimental variables resources EFO; EFO_0006693
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM169437
GEO; GSM170708
GEO; GSM170709
GEO; GSM784199
GEO; GSM794289
GEO; GSM887432
GEO; GSM888512
GEO; GSM1670246
GEO; GSM1887910
GEO; GSM1887992
Polymorphism and mutation databases Cosmic; 753605
Cosmic; 755466
Cosmic; 897519
Cosmic; 903575
Cosmic; 1006527
Cosmic; 1021865
Cosmic; 1219103
Cosmic; 1995580
Cosmic; 2125210
IARC_TP53; 437
LiGeA; CCLE_268
Progenetix; CVCL_1565
Proteomic databases PRIDE; PXD011896
PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number46