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Cellosaurus NCI-H250 (CVCL_1554)

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Cell line name NCI-H250
Synonyms H250; H-250; NCIH250
Accession CVCL_1554
Resource Identification Initiative To cite this cell line use: NCI-H250 (RRID:CVCL_1554)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: African American.
Microsatellite instability: Instable (MSI-low) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; miRNA profiling; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Brain; UBERON=UBERON_0000955.
Sequence variations
  • Mutation; HGNC; HGNC:9884; RB1; Simple; p.Gly840fs (c.2518delG); Zygosity=Heterozygous (Cosmic-CLP=924241; DepMap=ACH-002171).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Cys277Phe (c.830G>T); ClinVar=VCV000458567; Zygosity=Homozygous (Cosmic-CLP=924241; DepMap=ACH-002171).
Genome ancestry Source: PubMed=30894373

Origin% genome
African91.03
Native American0.09
East Asian, North1.17
East Asian, South0
South Asian0
European, North0
European, South7.7
Disease Lung small cell carcinoma (NCIt: C4917)
Small cell lung cancer (ORDO: Orphanet_70573)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 34Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-5828; Cosmic-CLP=924241

Markers:
AmelogeninX,Y
CSF1PO13
D5S81811
D7S8208,11
D13S31712
D16S5399,13
TH019
TPOX8,11
vWA15,16

Run an STR similarity search on this cell line
Publications

PubMed=6272398; DOI=10.1126/science.6272398
Terry W. Moody, Candace Beebe Pert, Adi F. Gazdar, Desmond Niall Carney, John D. Minna;
High levels of intracellular bombesin characterize human small-cell lung carcinoma.
Science 214:1246-1248(1981)

PubMed=6713399
Steven T. Rosen, James L. Mulshine, Frank Cuttitta, Joseph Fedorko, Desmond Niall Carney, Adi F. Gazdar, John D. Minna;
Analysis of human small cell lung cancer differentiation antigens using a panel of rat monoclonal antibodies.
Cancer Res. 44:2052-2061(1984)

PubMed=2985257
Desmond Niall Carney, Adi F. Gazdar, Gerold Bepler, John G. Guccion, Paul J. Marangos, Terry W. Moody, Mark H. Zweig, John D. Minna;
Establishment and identification of small cell lung cancer cell lines having classic and variant features.
Cancer Res. 45:2913-2923(1985)

PubMed=2986243; DOI=10.1007/978-3-642-82372-5_4
Jacqueline Whang-Peng, Elaine C. Lee;
Cytogenetics of human small cell lung cancer.
Recent Results Cancer Res. 97:37-46(1985)

PubMed=8806092; DOI=10.1002/jcb.240630505
Ruby M. Phelps, Bruce E. Johnson, Daniel C. Ihde, Adi F. Gazdar, David P. Carbone, Patrick R. McClintock, Ritva Ilona Linnoila, Mary J. Matthews, Paul A. Bunn Jr., Desmond Niall Carney, John D. Minna, James L. Mulshine;
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. Suppl. 24:32-91(1996)

PubMed=8806103; DOI=10.1002/jcb.240630516
Bruce E. Johnson, Edward Russell, Alfreda M. Simmons, Ruby M. Phelps, Seth M. Steinberg, Daniel C. Ihde, Adi F. Gazdar;
MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.
J. Cell. Biochem. Suppl. 24:210-217(1996)

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Sanjay Modi, Akihito Kubo, Herbert Kazuto Oie, Amy B. Coxon, Ahad Rehmatulla, Frederic J. Kaye;
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa, Jonathan Hinton, Ciara Fahey ...Show all 22 authors... , Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=27247353; DOI=10.1093/jnci/djw122; PMCID=PMC6279282
Eric C. Polley, Mark W. Kunkel, David Evans, Thomas E. Silvers, Rene M. Delosh, Julie Laudeman, Chad Ogle, Russell Reinhart, Michael Selby, John Connelly, Erik Harris, Nicole Fer, Dmitriy Sonkin ...Show all 18 authors... , Gurmeet Kaur, Anne Monks, Shakun Malik, Joel Morris, Beverly A. Teicher; Show fewer authors
Small cell lung cancer screen of oncology drugs, investigational agents, and gene and microRNA expression.
J. Natl. Cancer Inst. 108:djw122.1-djw122.11(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer, Patricia Greninger, Ewald van Dyk ...Show all 39 authors... , Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28766886; DOI=10.1002/cam4.1131; PMCID=PMC5548882
Beverly A. Teicher, Thomas E. Silvers, Michael Selby, Rene M. Delosh, Julie Laudeman, Chad Ogle, Russell Reinhart, Ralph E. Parchment, Julia Krushkal, Dmitriy Sonkin, Lawrence Rubinstein, Joel Morris, David Evans; Show fewer authors ...Show all 13 authors...
Small cell lung carcinoma cell line screen of etoposide/carboplatin plus a third agent.
Cancer Med. 6:1952-1964(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31803961; DOI=10.1002/jcb.29564; PMCID=PMC7496084
James L. Mulshine, Peter Ujhazy, Melissa Antman, Christine M. Burgess, Igor A. Kuzmin, Paul A. Bunn Jr., Bruce E. Johnson, Jack Alan Roth, Harvey I. Pass, Sheila M. Ross, Carolyn R. Aldige, Ignacio Ivan Wistuba, John D. Minna; Show fewer authors ...Show all 13 authors...
From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.
J. Cell. Biochem. 121:3986-3999(2020)

PubMed=32586373; DOI=10.1186/s13148-020-00876-8; PMCID=PMC7318526
Julia Krushkal, Thomas E. Silvers, William C. Reinhold, Dmitriy Sonkin, Suleyman Vural, John Connelly, Sudhir Varma, Paul S. Meltzer, Mark W. Kunkel, Annamaria Rapisarda, David Evans, Yves Pommier, Beverly A. Teicher; Show fewer authors ...Show all 13 authors...
Epigenome-wide DNA methylation analysis of small cell lung cancer cell lines suggests potential chemotherapy targets.
Clin. Epigenetics 12:93.1-93.28(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker, Jennifer Koh, Howard Lightfoot ...Show all 32 authors... , Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-5828
Cell line databases/resources CLO; CLO_0008078
cancercelllines; CVCL_1554
Cell_Model_Passport; SIDM00721
Cosmic-CLP; 924241
DepMap; ACH-002171
Biological sample resources BioSample; SAMN03471747
Chemistry resources ChEMBL-Cells; CHEMBL3308294
ChEMBL-Targets; CHEMBL2366289
GDSC; 924241
PharmacoDB; NCIH250_1094_2019
PubChem_Cell_line; CVCL_1554
Encyclopedic resources Wikidata; Q54907978
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM1670235
GEO; GSM1887908
GEO; GSM1887990
Polymorphism and mutation databases Cosmic; 688020
Cosmic; 924241
Cosmic; 1021858
IARC_TP53; 27193
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number40