Cellosaurus logo
expasy logo

Cellosaurus KYSE-30 (CVCL_1351)

[Text version]
Cell line name KYSE-30
Synonyms Kyse-30; KYSE 30; KYSE30; Kyse30; KYSE0030
Accession CVCL_1351
Resource Identification Initiative To cite this cell line use: KYSE-30 (RRID:CVCL_1351)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: KuDOS 95 cell line panel.
Population: Japanese.
Doubling time: 20.8 hours (PubMed=1728357); 22 hours (PubMed=25984343); ~30 hours (DSMZ=ACC-351).
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Phenotyping; shRNA library screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Misspelling: KYSE-300; Cosmic=801333.
Derived from site: In situ; Esophagus; UBERON=UBERON_0001043.
Sequence variations
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Glu120Ter (c.358G>T); Zygosity=Unspecified (PubMed=9033652; DepMap=ACH-000777).
  • Mutation; HGNC; HGNC:5173; HRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000376033; Zygosity=Unspecified (DepMap=ACH-000777).
  • Mutation; HGNC; HGNC:16051; PARD3; Unexplicit; Ex3-22del; Zygosity=Homozygous (PubMed=20215515).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; c.673-2A>G (IVS6-2A>G); ClinVar=VCV000458555; Zygosity=Unspecified; Note=Splice acceptor mutation (PubMed=8575860).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*02:06,24:02
HLA-BB*15:58,54:01
HLA-CC*04:01,08:03
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North75.07
East Asian, South24.93
South Asian0
European, North0
European, South0
Disease Esophageal squamous cell carcinoma (NCIt: C4024)
Squamous cell carcinoma of the esophagus (ORDO: Orphanet_99977)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_E3F6 (KYSE-30-EGFP)CVCL_E0WW (Ubigene KYSE-30 CCHCR1 KO)CVCL_E0WX (Ubigene KYSE-30 CYP26B1 KO)
CVCL_E0WY (Ubigene KYSE-30 DDAH2 KO)CVCL_E0WZ (Ubigene KYSE-30 FASN KO)CVCL_E0X0 (Ubigene KYSE-30 MCM10 KO)
CVCL_E0X1 (Ubigene KYSE-30 TNXB KO)CVCL_E0X2 (Ubigene KYSE-30 ZNF750 KO)
Sex of cell Male
Age at sampling 64Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-351; JCRB=JCRB0188; PubMed=25877200

Markers:
AmelogeninX
CSF1PO10
D2S133823
D3S135815,16
D5S81811
D7S82011,11.3 (JCRB=JCRB0188; PubMed=25877200)
11,12 (DSMZ=ACC-351)
D8S117912,15
D13S3179
D16S53910,12
D18S5114
D19S43315,16
D21S1128
FGA24
Penta D12
Penta E13
TH019
TPOX8,9 (JCRB=JCRB0188)
9 (DSMZ=ACC-351; PubMed=25877200)
vWA16,18 (DSMZ=ACC-351)
16,18,19 (JCRB=JCRB0188; PubMed=25877200)

Run an STR similarity search on this cell line
Publications

PubMed=1728357; DOI=10.1002/1097-0142(19920115)69:2<277::AID-CNCR2820690202>3.0.CO;2-C
Yutaka Shimada, Masayuki Imamura, Takashi Wagata, Nozomi Yamaguchi, Takayoshi Tobe;
Characterization of 21 newly established esophageal cancer cell lines.
Cancer 69:277-284(1992)

PubMed=7913084; DOI=10.1002/ijc.2910580224
Yuji Kanda, Yasuyuki Nishiyama, Yutaka Shimada, Masayuki Imamura, Hiroshi Nomura, Hiroshi Hiai, Manabu Fukumoto;
Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines.
Int. J. Cancer 58:291-297(1994)

PubMed=8575860; DOI=10.1002/(SICI)1097-0215(19960126)65:3<372::AID-IJC16>3.0.CO;2-C
Hisashi Tanaka, Ichio Shibagaki, Yutaka Shimada, Takashi Wagata, Masayuki Imamura, Kanji Ishizaki;
Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.
Int. J. Cancer 65:372-376(1996)

PubMed=9033652; DOI=10.1002/(SICI)1097-0215(19970207)70:4<437::AID-IJC11>3.0.CO;2-C
Hisashi Tanaka, Yutaka Shimada, Masayuki Imamura, Ichio Shibagaki, Kanji Ishizaki;
Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines.
Int. J. Cancer 70:437-442(1997)

PubMed=11092977; DOI=10.1111/j.1349-7006.2000.tb00895.x; PMCID=PMC5926289
Atiphan Pimkhaokham, Yutaka Shimada, Yohji Fukuda, Naoki Kurihara, Issei Imoto, Zeng-Quan Yang, Masayuki Imamura, Yusuke Nakamura, Teruo Amagasa, Johji Inazawa;
Nonrandom chromosomal imbalances in esophageal squamous cell carcinoma cell lines: possible involvement of the ATF3 and CENPF genes in the 1q32 amplicon.
Jpn. J. Cancer Res. 91:1126-1133(2000)

PubMed=11520067; DOI=10.1006/bbrc.2001.5400
Takatsugu Kan, Yutaka Shimada, Fumiaki Sato, Masato Maeda, Atsushi Kawabe, Jun-ichi Kaganoi, Atsushi Itami, Seiji Yamasaki, Masayuki Imamura;
Gene expression profiling in human esophageal cancers using cDNA microarray.
Biochem. Biophys. Res. Commun. 286:792-801(2001)

PubMed=12963126; DOI=10.1016/S0304-3835(03)00344-6
Mohammad Obaidul Hoque, Shahnaz Begum, Matthias Sommer, Taekyeol Lee, Barry Trink, Edward Ratovitski, David Sidransky;
PUMA in head and neck cancer.
Cancer Lett. 199:75-81(2003)

PubMed=15172977; DOI=10.1158/0008-5472.CAN-04-0172
Itaru Sonoda, Issei Imoto, Jun Inoue, Tatsuhiro Shibata, Yutaka Shimada, Koei Chin, Masayuki Imamura, Teruo Amagasa, Joe W. Gray, Setsuo Hirohashi, Johji Inazawa;
Frequent silencing of low density lipoprotein receptor-related protein 1B (LRP1B) expression by genetic and epigenetic mechanisms in esophageal squamous cell carcinoma.
Cancer Res. 64:3741-3747(2004)

PubMed=16045545; DOI=10.1111/j.0959-9673.2005.00431.x; PMCID=PMC2517430
Sadayuki Ban, Yuichi Michikawa, Ken-ichi Ishikawa, Masashi Sagara, Koji Watanabe, Yutaka Shimada, Johji Inazawa, Takashi Imai;
Radiation sensitivities of 31 human oesophageal squamous cell carcinoma cell lines.
Int. J. Exp. Pathol. 86:231-240(2005)

PubMed=16364037; DOI=10.1111/j.1442-2050.2006.00530.x
M. Su, Suet-Feung Chin, X.-Y. Li, Paul A.W. Edwards, Carlos Caldas, Rebecca Clare Fitzgerald;
Comparative genomic hybridization of esophageal adenocarcinoma and squamous cell carcinoma cell lines.
Dis. Esophagus 19:10-14(2006)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=21191746; DOI=10.1007/s11684-010-0260-x
Jun-Fang Ji, Kun Wu, Min Wu, Qi-Min Zhan;
p53 functional activation is independent of its genotype in five esophageal squamous cell carcinoma cell lines.
Front. Med. China 4:412-418(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
Glenn S. Cowley, Barbara A. Weir, Francisca Vazquez, Pablo Tamayo, Justine A. Scott, Scott Rusin, Alexandra East-Seletsky, Levi D. Ali, William F.J. Gerath, Sarah E. Pantel, Patrick Hall Lizotte ...Show all 40 authors... , Guo-Zhi Jiang, Jessica Hsiao, Aviad Tsherniak, Elizabeth Dwinell, Simon Aoyama, Michael Okamoto, William Harrington, Ellen T. Gelfand, Thomas M. Green, Mark J. Tomko, Shuba Gopal, Terence C. Wong, Hu-Bo Li, Sara Howell, Nicolas Stransky, Ted Liefeld, Dongkeun Jang, Jonathan Bistline, Barbara Hill Meyers, Scott Allen Armstrong, Kenneth Carl Anderson, Kimberly Stegmaier, Michael Reich, David Pellman, Jesse S. Boehm, Jill P. Mesirov, Todd Robert Golub, David E. Root, William Chun Hahn; Show fewer authors
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035.1-140035.12(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
David Paul Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald 3rd, Marian Kalocsay, Judit Jane-Valbuena, Ellen T. Gelfand, Devin K. Schweppe, Mark P. Jedrychowski, Javad Golji ...Show all 20 authors... , Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi Alexander Garraway, William Raj Sellers, Steven P. Gygi; Show fewer authors
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

Cross-references
Cell line collections (Providers) BCRJ; 0404
CLS; 305094
DSMZ; ACC-351
ECACC; 94072011
ICLC; HTL97022
JCRB; JCRB0188
NCBI_Iran; C584
Ubigene; YC-C054
Cell line databases/resources CLO; CLO_0007132
CLDB; cl3073
CLDB; cl3074
cancercelllines; CVCL_1351
Cell_Model_Passport; SIDM00015
CGH-DB; 274-1
CGH-DB; 9223-4
DepMap; ACH-000777
DSMZCellDive; ACC-351
IGRhCellID; KYSE30
LINCS_LDP; LCL-1553
Anatomy/cell type resources BTO; BTO_0004461
Biological sample resources BioSample; SAMN03471050
BioSample; SAMN03471648
BioSample; SAMN03473314
BioSample; SAMN10988382
CRISP screens repositories BioGRID_ORCS_Cell_line; 411
Chemistry resources ChEMBL-Cells; CHEMBL4513120
ChEMBL-Targets; CHEMBL4513130
GDSC; 1298221
PharmacoDB; KYSE30_806_2019
PubChem_Cell_line; CVCL_1351
Encyclopedic resources Wikidata; Q54900823
Experimental variables resources EFO; EFO_0002223
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
GEO; GSM827542
GEO; GSM887251
GEO; GSM888326
GEO; GSM1374609
GEO; GSM1374610
GEO; GSM1374611
Polymorphism and mutation databases Cosmic; 801333
Cosmic; 918521
Cosmic; 1123333
Cosmic; 1298221
Cosmic; 1339921
Cosmic; 1581068
Cosmic; 1876249
Cosmic; 2267697
Cosmic; 2395004
Cosmic; 2650627
Cosmic; 2698430
IARC_TP53; 7691
LiGeA; CCLE_634
Progenetix; CVCL_1351
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number46