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Cellosaurus Karpas-45 (CVCL_1326)

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Cell line name Karpas-45
Synonyms KARPAS-45; Karpas 45; KARPAS 45; KARPAS45; K45; T-45; T45; Line 45
Accession CVCL_1326
Resource Identification Initiative To cite this cell line use: Karpas-45 (RRID:CVCL_1326)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 50-100 hours (ECACC=06072602).
Microsatellite instability: Stable (MSS) (Sanger).
Microsatellite instability: Instable (MSI) (PubMed=31068700).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Gene fusion; HGNC; HGNC:7139; FOXO4 + HGNC; HGNC:7132; KMT2A; Name(s)=KMT2A-FOXO4, MLL-MLLT7, MLL-AFX1 (PubMed=14671638; PubMed=15843827).
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Val2194Ala (c.6581T>C); ClinVar=VCV000565658; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:3430; ERBB2; Simple; p.Ala586Gly (c.1757C>G); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:3495; ETV6; Simple; p.His328Tyr (c.982C>T); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:3689; FGFR2; Simple; p.Met584Arg (c.1751T>G) (p.Met585Arg, c.1754T>G); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:6307; KDR; Simple; p.Arg962His (c.2885G>A); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:6342; KIT; Simple; p.Asp131Asn (c.391G>A); ClinVar=VCV000409719; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:7029; MET; Simple; p.Ala48Thr (c.142G>A); ClinVar=VCV000454188; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:7029; MET; Simple; p.Glu157Gly (c.470A>G); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:7325; MSH2; Simple; p.Gly221Val (c.662G>T); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Asp1852Asn (c.5554G>A); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Val2045Ile (c.6133G>A); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Gln2459Ter (c.7375C>T); Zygosity=Heterozygous (PubMed=15472075).
  • Mutation; HGNC; HGNC:8804; PDGFRB; Simple; p.His277Gln (c.831C>A); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:9967; RET; Simple; p.Asn879Ser (c.2636A>G); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:11103; SMARCB1; Simple; p.Asn288Lys (c.864C>A); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273Cys (c.817C>T); ClinVar=VCV000043594; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; HGNC:12796; WT1; Simple; p.Arg394Gln (c.1181G>A) (p.Arg467Gln, c.1400G>A); ClinVar=VCV000419332; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.06
Native American0.47
East Asian, North0.03
East Asian, South0
South Asian2.75
European, North68.54
European, South28.16
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 2Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=907272; DSMZ=ACC-105

Markers:
AmelogeninX,Y
CSF1PO11,14
D2S133817,26
D3S135814,15
D5S81811,12
D7S8208,11
D8S117911,15
D13S3179,14
D16S5399,13
D18S5116,18
D19S43314
D21S1130,31.2
FGA22,23
Penta D12,13
Penta E5,10
TH019
TPOX8
vWA14,18

Run an STR similarity search on this cell line
Web pages Info; T-ALL cell lines db; -; https://humantallcelllines.wordpress.com/additionalcells/karpas-45/
Publications

DOI=10.1016/0145-2126(77)90063-7
Abraham Karpas, Frank George James Hayhoe, Joel S. Greenberger, Christopher R. Barker, Jerome C. Cawley, Ray M. Lowenthal, William Curry Moloney;
The establishment and cytological, cytochemical and immunological characterisation of human haemic cell lines: evidence for heterogeneity.
Leuk. Res. 1:35-49(1977)

DOI=10.1007/978-1-4613-2823-0_29
Abraham Karpas;
Viruses and human leukemia in vitro.
(In book chapter) Human leukemia. Cytochemical and ultrastructural techniques in diagnosis and research. Developments in Oncology, Vol. 14; Polliack, Aaron (eds.); pp.495-526; Martinus Nijhoff Publishing; Boston; USA (1984)

PubMed=3264071; DOI=10.1073/pnas.85.23.9229; PMCID=PMC282712
Abraham Karpas, George William John Fleet, Raymond Allen Dwek, Sigthor Petursson, Sung K. Namgoong, Nigel G. Ramsden, Gary S. Jacob, Thomas W. Rademacher;
Aminosugar derivatives as potential anti-human immunodeficiency virus agents.
Proc. Natl. Acad. Sci. U.S.A. 85:9229-9233(1988)

PubMed=9933131; DOI=10.1016/S0145-2126(98)00133-7
Renate Burger, Thomas E. Hansen-Hagge, Hans Gunther Drexler, Martin Gramatzki;
Heterogeneity of T-acute lymphoblastic leukemia (T-ALL) cell lines: suggestion for classification by immunophenotype and T-cell receptor studies.
Leuk. Res. 23:19-27(1999)

DOI=10.1016/B978-0-12-221970-2.50457-5
Hans Gunther Drexler;
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=14671638; DOI=10.1038/sj.leu.2403236
Hans Gunther Drexler, Hilmar Quentmeier, Roderick A.F. MacLeod;
Malignant hematopoietic cell lines: in vitro models for the study of MLL gene alterations.
Leukemia 18:227-232(2004)

PubMed=15472075; DOI=10.1126/science.1102160
Andrew P. Weng, Adolfo A. Ferrando, Woojoong Lee, John P. Morris 4th, Lewis B. Silverman, Cheryll Sanchez-Irizarry, Stephen Charles Blacklow, Alfred Thomas Look, Jon Christopher Aster;
Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.
Science 306:269-271(2004)

PubMed=15843827; DOI=10.1038/sj.leu.2403749
Anna K. Hagstrom-Andersson, Patrik Eden, David Lindgren, Jens Nilsson, Carin Lassen, Jesper Heldrup, Magnus Fontes, Ake Borg, Felix Mitelman, Bertil Johansson, Mattias Hoglund, Thoas Fioretos;
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Leukemia 19:1042-1050(2005)

PubMed=16408098; DOI=10.1038/sj.leu.2404081
Hilmar Quentmeier, Roderick A.F. MacLeod, Margarete Zaborski, Hans Gunther Drexler;
JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Leukemia 20:471-476(2006)

PubMed=17170727; DOI=10.1038/sj.leu.2404486
Yorick Sandberg, Brenda Verhaaf, Ellen J. van Gastel-Mol, Ingrid L.M. Wolvers-Tettero, John De Vos, Roderick A.F. MacLeod, Jeroen G. Noordzij, Willem A. Dik, Jack J.M. van Dongen, Anton W. Langerak;
Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes.
Leukemia 21:230-237(2007)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=22675565; DOI=10.1371/journal.pone.0038463; PMCID=PMC3366948
Zeynep Kalender Atak, Kim De Keersmaecker, Valentina Gianfelici, Ellen Geerdens, Roel Vandepoel, Daphnie Pauwels, Michael Porcu, Idoya Lahortiga, Vanessa Brys, Wilhelm Gerhard Dirks, Hilmar Quentmeier ...Show all 17 authors... , Jacqueline Cloos, Harry Cuppens, Anne Uyttebroeck, Peter Vandenberghe, Jan Cools, Stein Aerts; Show fewer authors
High accuracy mutation detection in leukemia on a selected panel of cancer genes.
PLoS ONE 7:e38463.1-e38463.11(2012)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=35354797; DOI=10.1038/s41467-022-29224-5; PMCID=PMC8967900
Isabelle Rose Leo, Luay Aswad, Matthias Stahl, Elena Kunold, Frederik Post, Tom Erkers, Nona Struyf, Georgios Mermelekas, Rubin Narayan Joshi, Eva Gracia-Villacampa, Paivi Ostling ...Show all 17 authors... , Olli-Pekka Kallioniemi, Katja Pokrovskaja Tamm, Ioannis Siavelis, Janne Lehtio, Mattias Vesterlund, Rozbeh Jafari; Show fewer authors
Integrative multi-omics and drug response profiling of childhood acute lymphoblastic leukemia cell lines.
Nat. Commun. 13:1691.1-1691.19(2022)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) DSMZ; ACC-105 - Discontinued
ECACC; 06072602
NCBI_Iran; C180
Cell line databases/resources CLO; CLO_0007067
CLDB; cl35
cancercelllines; CVCL_1326
Cell_Model_Passport; SIDM01007
Cosmic-CLP; 907272
DepMap; ACH-002256
DSMZCellDive; ACC-105
LINCS_LDP; LCL-1015
Biological sample resources BioSample; SAMN03473409
Chemistry resources ChEMBL-Cells; CHEMBL3308305
ChEMBL-Targets; CHEMBL2366305
GDSC; 907272
PharmacoDB; KARPAS45_731_2019
PubChem_Cell_line; CVCL_1326
Encyclopedic resources Wikidata; Q54899501
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM1669977
GEO; GSM5137719
Polymorphism and mutation databases Cosmic; 907272
Cosmic; 913413
Cosmic; 1012075
Cosmic; 1151781
Cosmic; 1175130
Cosmic; 1191704
Cosmic; 1281370
Cosmic; 1330505
Cosmic; 1524799
Cosmic; 1664523
Cosmic; 1760525
Cosmic; 2165714
Cosmic; 2391812
Cosmic; 2602917
IARC_TP53; 27173
Proteomic databases PRIDE; PXD023662
PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number44