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Cellosaurus HT-3 (CVCL_1293)

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Cell line name HT-3
Synonyms HT3
Accession CVCL_1293
Resource Identification Initiative To cite this cell line use: HT-3 (RRID:CVCL_1293)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK 784-01.
Population: Caucasian.
Doubling time: 2.48 days (PubMed=29156801).
Microsatellite instability: Stable (MSS) (Sanger).
Transformant: NCBI_TaxID; 10611; Human papillomavirus type 30 (HPV30).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Caution: Indicated as originating from a 58 year old female patient in ATCC and from a 53 year old in Sloan Kettering tech transfer site.
Derived from site: Metastatic; Lymph node; UBERON=UBERON_0000029.
Sequence variations
HLA typing Source: PubMed=77569
Class I
HLA-AA*03,28
HLA-BB*8,05/w35
HLA-CC*w02,w03
Genome ancestry Source: PubMed=30894373

Origin% genome
African2.04
Native American0
East Asian, North3.34
East Asian, South0
South Asian1.53
European, North55.35
European, South37.73
Disease Cervical carcinoma (NCIt: C9039)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-32; Cosmic-CLP=907068; DepMap=ACH-001525; PubMed=25877200

Markers:
AmelogeninX
CSF1PO12
D2S133821
D3S135815
D5S81810,13
D7S8208,10
D8S117913,14
D13S31712
D16S53912,13
D18S5115
D19S43314
D21S1129
FGA21,22
Penta D12
Penta E11,12
TH016,7
TPOX8
vWA15,18

Run an STR similarity search on this cell line
Web pages Provider; MSKCC; -; https://www.mskcc.org/research-advantage/support/technology/tangible-material/ht-3-human-cervical-cell-line
Publications

DOI=10.1007/978-1-4757-1647-4_5
Jorgen Fogh, Germain L. Trempe;
New human tumor cell lines.
(In book chapter) Human tumor cells in vitro; Fogh, Jorgen (eds.); pp.115-159; Springer; New York; USA (1975)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Jorgen Fogh, Jens M. Fogh, Thomas Orfeo;
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Jorgen Fogh, William C. Wright, James D. Loveless;
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=924690; DOI=10.1002/ijc.2910200505
Robert S. Kerbel, Hugh F. Pross, Albert Leibovitz;
Analysis of established human carcinoma cell lines for lymphoreticular-associated membrane receptors.
Int. J. Cancer 20:673-679(1977)

PubMed=77569; DOI=10.1111/j.1399-0039.1978.tb01259.x
Jons Ake Espmark, Lena Ahlqvist-Roth, Lena Sarne, Anita Persson;
Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.
Tissue Antigens 11:279-286(1978)

PubMed=7459858
Monique Rousset, Alain Zweibaum, Jorgen Fogh;
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=6220172
Nicholas C. Dracopoli, Jorgen Fogh;
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=2990217; PMCID=PMC1888002
Carole Yee, Indira Krishnan-Hewlett, Carl C. Baker, Richard Schlegel, Peter Maxwell Howley;
Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.
Am. J. Pathol. 119:361-366(1985)

PubMed=3518877; DOI=10.3109/07357908609038260
Jorgen Fogh;
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=1648218; DOI=10.1073/pnas.88.13.5523; PMCID=PMC51909
Martin Scheffner, Karl Munger, Janet C. Byrne, Peter Maxwell Howley;
The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines.
Proc. Natl. Acad. Sci. U.S.A. 88:5523-5527(1991)

PubMed=10392903
Lisa Kay Naeger, Edward C. Goodwin, Eun-Seong Hwang, Rosa Anna DeFilippis, Hui Zhang, Daniel DiMaio;
Bovine papillomavirus E2 protein activates a complex growth-inhibitory program in p53-negative HT-3 cervical carcinoma cells that includes repression of cyclin A and cdc25A phosphatase genes and accumulation of hypophosphorylated retinoblastoma protein.
Cell Growth Differ. 10:413-422(1999)

PubMed=15531914; DOI=10.1038/sj.onc.1208235
Stefan E. Baldus, Elisabeth Schwarz, Claudia Lohrey, Marc Zapatka, Stephanie Landsberg, Stephan A. Hahn, Dietmar Schmidt, Hans-Peter Dienes, Wolff Helmut Schmiegel, Irmgard Schwarte-Waldhoff;
Smad4 deficiency in cervical carcinoma cells.
Oncogene 24:810-819(2005)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=24134916; DOI=10.1186/1755-8166-6-44; PMCID=PMC3879223
Amanda McCormack, Jiang-Lan Fan, Max Duesberg, Mathew Bloomfield, Christian Fiala, Peter Heinz Duesberg;
Individual karyotypes at the origins of cervical carcinomas.
Mol. Cytogenet. 6:44.1-44.23(2013)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=29156801; DOI=10.18632/oncotarget.21174; PMCID=PMC5689691
Nene N. Kalu, Tuhina Mazumdar, Shao-Hua Peng, Li Shen, Vaishnav Sambandam, Xia-Yu Rao, Yuan-Xin Xi, Le-Rong Li, Yuan Qi, Frederico O. Gleber-Netto, Ameeta Patel ...Show all 16 authors... , Jing Wang, Mitchell J. Frederick, Jeffrey N. Myers, Curtis R. Pickering, Faye M. Johnson; Show fewer authors
Genomic characterization of human papillomavirus-positive and -negative human squamous cell cancer cell lines.
Oncotarget 8:86369-86383(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; HTB-32
CancerTools; 161930
Cell line databases/resources CLO; CLO_0004286
cancercelllines; CVCL_1293
Cell_Model_Passport; SIDM00679
Cosmic-CLP; 907068
DepMap; ACH-001525
IGRhCellID; HT3
LINCS_LDP; LCL-1304
Biological sample resources BioSample; SAMN03472916
Chemistry resources ChEMBL-Cells; CHEMBL3308745
ChEMBL-Targets; CHEMBL1075470
GDSC; 907068
PharmacoDB; HT3_631_2019
PubChem_Cell_line; CVCL_1293
Encyclopedic resources Wikidata; Q54896535
Experimental variables resources EFO; EFO_0002204
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM253299
GEO; GSM675735
GEO; GSM685442
GEO; GSM1669914
Polymorphism and mutation databases Cosmic; 760222
Cosmic; 809227
Cosmic; 846183
Cosmic; 907068
Cosmic; 925353
Cosmic; 928908
Cosmic; 1131521
Cosmic; 1193036
Cosmic; 1201774
Cosmic; 1995449
Cosmic; 2301540
Cosmic; 2660191
Cosmic; 2816219
IARC_TP53; 325
Progenetix; CVCL_1293
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number39