Cellosaurus logo
expasy logo

Cellosaurus HEC-1 (CVCL_1274)

[Text version]
Cell line name HEC-1
Synonyms HEC1; Human Endometrial Cancer-1; HEC-B-296
Accession CVCL_1274
Resource Identification Initiative To cite this cell line use: HEC-1 (RRID:CVCL_1274)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: MD Anderson Cell Lines Project.
Population: Japanese.
Microsatellite instability: Instable (MSI-high) (PubMed=31068700; Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Endometrium; UBERON=UBERON_0001295.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (Cosmic-CLP=907051; DepMap=ACH-001517).
  • Mutation; HGNC; HGNC:7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Heterozygous (DepMap=ACH-001517).
  • Mutation; HGNC; HGNC:7329; MSH6; Simple; c.4001+1G>T; ClinVar=VCV001736936; Zygosity=Heterozygous; Note=Splice donor mutation (Cosmic-CLP=907051; DepMap=ACH-001517).
  • Mutation; HGNC; HGNC:9122; PMS2; Simple; p.Arg802Ter (c.2404C>T); ClinVar=VCV000009237; Zygosity=Heterozygous (DepMap=ACH-001517).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Homozygous (Cosmic-CLP=907051; DepMap=ACH-001517).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North89.14
East Asian, South10.41
South Asian0.45
European, North0
European, South0
Disease Type II endometrial adenocarcinoma (NCIt: C39749)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_0293 (HEC-1-A)CVCL_S835 (HEC-1C)CVCL_S836 (HEC-1D)
Sex of cell Female
Age at sampling 71Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=907051; DepMap=ACH-001517; JCRB=JCRB0042

Markers:
AmelogeninX
CSF1PO10,12
D3S135815
D5S81811,13
D7S82011
D8S117913,14
D13S31711 (JCRB=JCRB0042)
11,16 (Cosmic-CLP=907051; DepMap=ACH-001517)
D16S53911,12
D18S5116,20,21
D21S1130,31
FGA21,22
Penta D9,13
Penta E11
TH016,7
TPOX11
vWA18

Run an STR similarity search on this cell line
Web pages Info; MCLP; -; https://tcpaportal.org/mclp/
Publications

PubMed=4673779; DOI=10.1016/0002-9378(72)90861-7
Hiroyuki Kuramoto, Shozo Tamura, Yukio Notake;
Establishment of a cell line of human endometrial adenocarcinoma in vitro.
Am. J. Obstet. Gynecol. 114:1012-1019(1972)

PubMed=4678779
Hiroyuki Kuramoto;
Studies of the growth and cytogenetic properties of human endometrial adenocarcinoma in culture and its development into an established line.
Acta Obstet. Gynaecol. Jpn. 19:47-58(1972)

PubMed=7448794
David Gal, Paul Cloren MacDonald, John Charles Porter, Jesse W. Smith, Evan R. Simpson;
Effect of cell density and confluency on cholesterol metabolism in cancer cells in monolayer culture.
Cancer Res. 41:473-477(1981)

PubMed=2064198; DOI=10.1111/j.1749-6632.1991.tb37884.x
Hiroyuki Kuramoto, Masato Nishida, Takayuki Morisawa, Mieko Hamano, Hiroki Hata, Yoshiki Kato, Eiji Ohno, Tomohiro Iida;
Establishment and characterization of human endometrial cancer cell lines.
Ann. N. Y. Acad. Sci. 622:402-421(1991)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Li-Qun Jia, Motonobu Osada, Chikashi Ishioka, Makio Gamo, Shuntaro Ikawa, Takao Suzuki, Hideki Shimodaira, Tomohito Niitani, Toshio Kudo, Mitoshi Akiyama, Narimiti Kimura ...Show all 18 authors... , Mitsuyoshi Matsuo, Hiroshi Mizusawa, Noriho Tanaka, Hideki Koyama, Masayoshi Namba, Ryunosuke Kanamaru, Toshio Kuroki; Show fewer authors
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=12227503; DOI=10.1111/j.1749-0774.2002.tb00103.x
Hiroyuki Kuramoto, Mieko Hamano, Manami Imai;
HEC-1 cells.
Hum. Cell 15:81-95(2002)

DOI=10.1007/978-4-431-53981-0_1
Hiroyuki Kuramoto, Mieko Hamano, Manami Imai, Takesi Fujisawa, Yuko Kamata, Tsutomu Arai, Miwa Kawaguchi;
HEC-1 cells: establishment of an in vitro experimental system in endometrial carcinoma.
(In book chapter) Cell and molecular biology of endometrial carcinoma; Kuramoto, Hiroyuki & Nishida, Masato (eds.); pp.3-34; Springer; Tokyo; Japan (2003)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) JCRB; JCRB0042
Cell line databases/resources CLO; CLO_0037162
cancercelllines; CVCL_1274
Cell_Model_Passport; SIDM00596
Cosmic-CLP; 907051
DepMap; ACH-001517
LINCS_HMS; 50014
LINCS_LDP; LCL-1496
Biological sample resources BioSample; SAMN03472810
CRISP screens repositories BioGRID_ORCS_Cell_line; 867
Chemistry resources ChEMBL-Cells; CHEMBL3308740
ChEMBL-Targets; CHEMBL1075462
GDSC; 907051
PharmacoDB; HEC1_523_2019
PubChem_Cell_line; CVCL_1274
Encyclopedic resources Wikidata; Q54882245
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM827285
GEO; GSM1669873
Polymorphism and mutation databases Cosmic; 907051
IARC_TP53; 21367
Progenetix; CVCL_1274
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number39