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Cellosaurus HCE-T (CVCL_1272)

[Text version]
Cell line name HCE-T
Synonyms HCET; Human Corneal Epithelial cells-Transformed; HCE; SV40-HCEC
Accession CVCL_1272
Resource Identification Initiative To cite this cell line use: HCE-T (RRID:CVCL_1272)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Japanese.
Doubling time: 24.4 hours (PubMed=7534282).
Transformant: Ad-SV40 hybrid virus.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Eye, cornea, epithelium; UBERON=UBERON_0001772.
Cell type: Epithelial cell of cornea; CL=CL_0000575.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 49Y
Category Transformed cell line
STR profile Source(s): PubMed=26900324; PubMed=29787296; RCB=RCB2280

Markers:
AmelogeninX
CSF1PO9,12 (PubMed=26900324)
12 (RCB=RCB2280; PubMed=29787296)
D2S133819,20
D3S135815,17
D5S8189,13 (PubMed=26900324; PubMed=29787296)
13 (RCB=RCB2280)
D7S82010,13
D8S117911,13
D13S3178
D16S5399,11
D18S5113 (PubMed=29787296)
13,15 (PubMed=26900324)
D19S43313,14
D21S1127,30
FGA20
Penta D12,15
Penta E15,16
TH017,8
TPOX8,11
vWA18,19

Run an STR similarity search on this cell line
Publications

PubMed=7534282
Kaoru Araki-Sasaki, Yuichi Ohashi, Tetsuo Sasabe, Kozaburo Hayashi, Hitoshi Watanabe, Yasuo Tano, Hiroshi Handa;
An SV40-immortalized human corneal epithelial cell line and its characterization.
Invest. Ophthalmol. Vis. Sci. 36:614-621(1995)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=26900324; PMCID=PMC4734150
Debra Akin, Jeremy R.B. Newman, Lauren M. McIntyre, Stephen P. Sugrue;
RNA-seq analysis of impact of PNN on gene expression and alternative splicing in corneal epithelial cells.
Mol. Vis. 22:40-60(2016)

PubMed=28315467; DOI=10.1016/j.ejps.2017.03.018
Marina Juretic, Bisera Jurisic Dukovski, Iva Krtalic, Stephan Reichl, Biserka Cetina-Cizmek, Jelena Filipovic-Grcic, Jasmina Lovric, Ivan Pepic;
HCE-T cell-based permeability model: a well-maintained or a highly variable barrier phenotype?
Eur. J. Pharm. Sci. 104:23-30(2017)

DOI=10.22028/D291-35665
Anna-Klara Rubelowski;
Characterization of the HCE-T cell line (RCB 2280) and its comparison with primary limbal epithelial cell culture and primary corneal epithelial cells.
Thesis MD (2018); Saarland University; Saarbrucken; Germany

PubMed=29787296; DOI=10.1080/02713683.2018.1480043; PMCID=PMC6590053
Alison M. McDermott, Hasna Baidouri, Ashley M. Woodward, Wendy R. Kam, Yang Liu, Xiao-Min Chen, Jillian F. Ziemanski, Kerry Vistisen, Linda D. Hazlett, Kelly K. Nichols, Pablo Argueso, David A. Sullivan;
Short tandem repeat (STR) profiles of commonly used human ocular surface cell lines.
Curr. Eye Res. 43:1097-1101(2018)

PubMed=31927639; DOI=10.1007/s00417-019-04563-0
Anna-Klara Rubelowski, Lorenz Latta, Priya Katiyar, Tanja Stachon, Barbara Kasmann-Kellner, Berthold Seitz, Nora Szentmary;
HCE-T cell line lacks cornea-specific differentiation markers compared to primary limbal epithelial cells and differentiated corneal epithelium.
Graefes Arch. Clin. Exp. Ophthalmol. 258:565-575(2020)

Cross-references
Cell line collections (Providers) BCRJ; 0349 - Discontinued
CLS; 305255
RCB; RCB1384 - Discontinued
RCB; RCB2280
Cell line databases/resources CLO; CLO_0050405
CLO; CLO_0050406
Cell_Model_Passport; SIDM00075
DepMap; ACH-002336
FCS-free; 4-2-4-2-4-3
FCS-free; 4-2-6-1-4-3
LINCS_LDP; LCL-1047
Anatomy/cell type resources BTO; BTO_0006396
Biological sample resources BioSample; SAMN03472134
Chemistry resources ChEMBL-Cells; CHEMBL3308200
ChEMBL-Targets; CHEMBL2366139
PharmacoDB; HCET_516_2019
PubChem_Cell_line; CVCL_1272
Encyclopedic resources Wikidata; Q54881856
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSE73060
GEO; GSM1669867
Polymorphism and mutation databases Cosmic; 907049
IARC_TP53; 27695
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number31