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Cellosaurus GI-ME-N (CVCL_1232)

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Cell line name GI-ME-N
Synonyms Gi-ME-N; Gi-MEN; GIMEN; Gimen; Gimen1; Gaslini Institute-ME-Neuroblastoma
Accession CVCL_1232
Resource Identification Initiative To cite this cell line use: GI-ME-N (RRID:CVCL_1232)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: ~25 hours (CLS=300179); ~24 hours (DSMZ=ACC-654).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Bone marrow; UBERON=UBERON_0002371.
Sequence variations
  • Gene deletion; HGNC; HGNC:7765; NF1; Zygosity=Heterozygous (PubMed=20655465).
  • Mutation; HGNC; HGNC:7765; NF1; Unexplicit; Microdeletion; Zygosity=Heterozygous (PubMed=20655465).
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.35
Native American0.42
East Asian, North0.23
East Asian, South0
South Asian7.63
European, North34.54
European, South55.82
Disease Neuroblastoma (NCIt: C3270)
Neuroblastoma (ORDO: Orphanet_635)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_RS22 (GI-ME-NrCDDP500)CVCL_RS07 (GI-ME-NrDOX5)CVCL_RR32 (GI-ME-NrVCR14)
Sex of cell Female
Age at sampling 3Y6M
Category Cancer cell line
STR profile Source(s): CLS=300179; Cosmic-CLP=906872; DepMap=ACH-001344; DSMZ=ACC-654

Markers:
AmelogeninX
CSF1PO11,12
D2S133819,20
D3S135814
D5S81812
D7S82010,11
D8S117910,14 (DepMap=ACH-001344)
11,15 (DSMZ=ACC-654)
D13S3178,12 (Cosmic-CLP=906872; DepMap=ACH-001344; DSMZ=ACC-654)
12 (CLS=300179)
D16S5399,12
D18S5112,17
D19S43313,14
D21S1131
FGA19,22
Penta D9,13
Penta E15,20 (DepMap=ACH-001344)
15,21 (DSMZ=ACC-654)
TH016,7
TPOX11
vWA16,19

Run an STR similarity search on this cell line
Publications

PubMed=3615018
Armando Melodia, Laura Cornara, Roberta Bertelli, Marco Canepa, Giorgio Gimelli, Guillermo Repetto, Paolo Cornaglia-Ferraris;
New line of human neuroblastoma derived from bone marrow.
Pathologica 78:371-384(1986)

PubMed=3204111; DOI=10.1007/BF00398190
Letizia Longo, Holger Christiansen, Nina M. Christiansen, Paolo Cornaglia-Ferraris, Fritz Lampert;
N-myc amplification at chromosome band 1p32 in neuroblastoma cells as investigated by in situ hybridization.
J. Cancer Res. Clin. Oncol. 114:636-640(1988)

PubMed=3406012
Mirco Ponzoni, Armando Melodia, Carmela Cirillo, Antonella Casalaro, Paolo Cornaglia-Ferraris;
Effect of cytosine arabinoside on the growth and phenotypic expression of GI-ME-N, a new human neuroblastoma cell line.
Prog. Clin. Biol. Res. 271:437-448(1988)

PubMed=3422578; DOI=10.1016/0165-4608(88)90188-4
Emilio Donti, Letizia Longo, Gian Paolo Tonini, Giovanna Verdona, Armando Melodia, Edoardo Lanino, Paolo Cornaglia-Ferraris;
Cytogenetic and molecular study of two human neuroblastoma cell lines.
Cancer Genet. Cytogenet. 30:225-231(1988)

PubMed=2535035; DOI=10.1002/gcc.2870010111
Tommy Martinsson, Andreas Weith, Celina Cziepluch, Manfred Schwab;
Chromosome 1 deletions in human neuroblastomas: generation and fine mapping of microclones from the distal 1p region.
Genes Chromosomes Cancer 1:67-78(1989)

PubMed=2917605; DOI=10.1016/0014-4827(89)90196-1
Mirco Ponzoni, Marina Lanciotti, Armando Melodia, Antonella Casalaro, Paolo Cornaglia-Ferraris;
Morphologic and phenotypic changes of human neuroblastoma cells in culture induced by cytosine arabinoside.
Exp. Cell Res. 181:226-237(1989)

PubMed=2296463; DOI=10.1203/00006450-199001000-00001
Paolo Cornaglia-Ferraris, Mirco Ponzoni, Paolo G. Montaldo, Gian Luigi Mariottini, Emilio Donti, Daniela Di Martino, Gian Paolo Tonini;
A new human highly tumorigenic neuroblastoma cell line with undetectable expression of N-myc.
Pediatr. Res. 27:1-6(1990)

DOI=10.1016/B978-0-12-333530-2.50006-X
Mark A. Israel, Carol Jeanne Thiele;
Tumor cell lines of the peripheral nervous system.
(In book chapter) Atlas of human tumor cell lines; Hay, Robert J. & Park, Jae-Gahb & Gazdar, Adi F. (eds.); pp.43-78; Academic Press; New York; USA (1994)

PubMed=7838528
Ngan Ching Cheng, Nadine Van Roy, Alvin Chan, Mabel Beitsma, Andries Westerveld, Frank Speleman, Rogier Versteeg;
Deletion mapping in neuroblastoma cell lines suggests two distinct tumor suppressor genes in the 1p35-36 region, only one of which is associated with N-myc amplification.
Oncogene 10:291-297(1995)

PubMed=9283597; DOI=10.1016/S0165-4608(96)00362-7
Nadine Van Roy, Anna Jauch, Mireille Van Gele, Genevieve Laureys, Rogier Versteeg, Anne De Paepe, Thomas Cremer, Frank Speleman;
Comparative genomic hybridization analysis of human neuroblastomas: detection of distal 1p deletions and further molecular genetic characterization of neuroblastoma cell lines.
Cancer Genet. Cytogenet. 97:135-142(1997)

PubMed=9516836; DOI=10.1016/S0959-8049(97)00319-5
Nadine Van Roy, Genevieve Laureys, Mireille Van Gele, Ghislain Opdenakker, R. Miura, Pauline van der Drift, Alvin Chan, Rogier Versteeg, Frank Speleman;
Analysis of 1;17 translocation breakpoints in neuroblastoma: implications for mapping of neuroblastoma genes.
Eur. J. Cancer 33:1974-1978(1997)

DOI=10.1007/0-306-46872-7_2
Carol Jeanne Thiele;
Neuroblastoma.
(In book chapter) Human cell culture. Vol. 1. Cancer cell lines part 1; Masters, John R.W. & Palsson, Bernhard Orn (eds.); pp.21-53; Kluwer Academic Publishers; New York; USA (1999)

PubMed=11550280; DOI=10.1002/gcc.1174
Nadine Van Roy, Heidi Van Limbergen, Jo Vandesompele, Mireille Van Gele, Bruce Poppe, Helen R. Salwen, Genevieve Laureys, Nunes Manoel, Anne De Paepe, Frank Speleman;
Combined M-FISH and CGH analysis allows comprehensive description of genetic alterations in neuroblastoma cell lines.
Genes Chromosomes Cancer 32:126-135(2001)

PubMed=17506115; DOI=10.1002/nbm.1181
Andrew C. Peet, Carmel M. McConville, Martin Paul Wilson, Barry A. Levine, Michelle Reed, Sara A. Dyer, Emma C. Edwards, Mary C. Strachan, Dominic J. McMullan, Timothy M. Wilkes, Richard G. Grundy;
1H MRS identifies specific metabolite profiles associated with MYCN-amplified and non-amplified tumour subtypes of neuroblastoma cell lines.
NMR Biomed. 20:692-700(2007)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20655465; DOI=10.1016/j.cell.2010.06.004; PMCID=PMC2913027
Michael Holzel, Si-Dong Huang, Jan Koster, Ingrid Ora, Arjan Lakeman, Huib Nicolaas Caron, Wouter Nijkamp, Jing Xie, Tom Callens, Shahab Asgharzadeh, Robert C. Seeger ...Show all 14 authors... , Ludwine M. Messiaen, Rogier Versteeg, Rene Bernards; Show fewer authors
NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome.
Cell 142:218-229(2010)

PubMed=22213050; DOI=10.1002/ijc.27415; PMCID=PMC3757132
Joanna E. Gawecka, Dirk Geerts, Jan Koster, Maisel J. Caliva, Florian J. Sulzmaier, John Opoku-Ansah, Randal K. Wada, Andre S. Bachmann, Joe W. Ramos;
PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.
Int. J. Cancer 131:1556-1568(2012)

PubMed=24466371; DOI=10.1593/tlo.13544; PMCID=PMC3890703
Nadine Loschmann, Martin Michaelis, Florian Rothweiler, Richard Zehner, Jaroslaw Cinatl, Yvonne Voges, Mohsen Sharifi, Kristoffer Riecken, Jochen Meyer, Andreas von Deimling, Iduna Fichtner ...Show all 14 authors... , Taravat Ghafourian, Frank Westermann, Jindrich Cinatl Jr.; Show fewer authors
Testing of SNS-032 in a panel of human neuroblastoma cell lines with acquired resistance to a broad range of drugs.
Transl. Oncol. 6:685-696(2013)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) CLS; 300179
DSMZ; ACC-654
ICLC; HTL98011
Cell line databases/resources CLO; CLO_0003494
CLDB; cl1464
CLDB; cl4931
cancercelllines; CVCL_1232
Cell_Model_Passport; SIDM00227
Cosmic-CLP; 906872
DepMap; ACH-001344
DSMZCellDive; ACC-654
LINCS_LDP; LCL-1983
Anatomy/cell type resources BTO; BTO_0005808
Biological sample resources BioSample; SAMN03473467
Chemistry resources ChEMBL-Cells; CHEMBL3308868
ChEMBL-Targets; CHEMBL2366074
GDSC; 906872
PharmacoDB; GIMEN_405_2019
PubChem_Cell_line; CVCL_1232
Encyclopedic resources Wikidata; Q54835813
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM692856
GEO; GSM1669810
GEO; GSM3145615
GEO; GSM3145705
Polymorphism and mutation databases Cosmic; 755616
Cosmic; 906872
Cosmic; 1167992
Cosmic; 1518065
Cosmic; 1526625
Cosmic; 1543771
Cosmic; 2239471
IARC_TP53; 23838
IARC_TP53; 27693
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number42