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Cellosaurus GCT (CVCL_1229)

[Text version]
Cell line name GCT
Synonyms Giant Cell Tumor; UR-HCL-1; University of Rochester-Human Cell Line-1
Accession CVCL_1229
Resource Identification Initiative To cite this cell line use: GCT (RRID:CVCL_1229)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Population: Caucasian.
Doubling time: 55 hours (PubMed=304748).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (PubMed=12068308; ATCC=TIB-223; Cosmic-CLP=906999; DepMap=ACH-000835).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Leu32Arg (c.95_96TG>GT); Zygosity=Homozygous (ATCC=TIB-223; Cosmic-CLP=906999; DepMap=ACH-000835).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg248Trp (c.742C>T); ClinVar=VCV000012347; Zygosity=Heterozygous (ATCC=TIB-223; Cosmic-CLP=906999; DepMap=ACH-000835).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln317Ter (c.948_949delinsTT) (c.948_949CC>TT); Zygosity=Heterozygous (ATCC=TIB-223; Cosmic-CLP=906999; DepMap=ACH-000835).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,23:01
HLA-BB*08:01,15:17
HLA-CC*07:01,07:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.07
Native American0
East Asian, North2.31
East Asian, South0
South Asian1.28
European, North63.83
European, South32.51
Disease Undifferentiated pleomorphic sarcoma (NCIt: C4247)
Undifferentiated pleomorphic sarcoma (ORDO: Orphanet_2023)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_E4CX (GCT-C)
Sex of cell Male
Age at sampling 29Y
Category Cancer cell line
STR profile Source(s): ATCC=TIB-223; CLS=300155; Cosmic-CLP=906999; IZSLER=BS TCL 94

Markers:
AmelogeninX
CSF1PO12
D2S133817,18
D3S135816,17
D5S81813,15
D7S82011,12
D8S117911,13
D13S31711,12
D16S5399
D18S5117,19
D19S43314
D21S1128
FGA21
Penta D12
Penta E12,13
TH018,9.3
TPOX8,9
vWA16,18

Run an STR similarity search on this cell line
Publications

PubMed=304748; DOI=10.1182/blood.V51.3.507.507
John F. DiPersio, James K. Brennan, Marshall Albert Lichtman, Burton Lyle Speiser;
Human cell lines that elaborate colon-stimulating activity for the marrow cells of man and other species.
Blood 51:507-519(1978)

PubMed=6299509; DOI=10.1016/0165-4608(83)90076-6
Frederick Hecht, Carol S. Berger, Barbara Kaiser-McCaw Hecht, Burton Lyle Speiser;
Fibrous histiocytoma cell line: chromosome studies of mouse and man.
Cancer Genet. Cytogenet. 8:359-362(1983)

PubMed=7689482
Jane L. Liesveld, Shelby Rush, Maureen C. Kempski, A. Robert Turner, James K. Brennan, Judith C. Gasson, Camille N. Abboud;
Phenotypic characterization of the human fibrous histiocytoma giant cell tumor (GCT) cell line and its cytokine repertoire.
Exp. Hematol. 21:1342-1352(1993)

PubMed=12068308; DOI=10.1038/nature00766
Helen R. Davies, Graham Robert Bignell, Charles Cox, Philip J. Stephens, Sarah Edkins, Sheila Clegg, Jon W. Teague, Hayley Woffendin, Mathew J. Garnett, William Bottomley, Neil Davis ...Show all 52 authors... , Ed Dicks, Rebecca Ewing, Yvonne Floyd, Kristian Gray, Sarah Hall, Rachel Hawes, Jaime Hughes, Vivian Kosmidou, Andrew Menzies, Catherine Mould, Adrian Parker, Claire Stevens, Stephen Watt, Steven Hooper, Rebecca Wilson, Hiran Jayatilake, Barry Austin Gusterson, Colin S. Cooper, Janet M. Shipley, Darren Hargrave, Kathy Pritchard-Jones, Norman J. Maitland, Georgia Chenevix-Trench, Gregory J. Riggins, Darell Doty Bigner, Giuseppe Palmieri, Antonio Cossu, Adrienne M. Flanagan, Andrew Nicholson, Judy W.C. Ho, Suet Yi Leung, Siu Tsan Yuen, Barbara L. Weber, Hilliard Foster Seigler, Timothy L. Darrow, Hugh F. Paterson, Richard Marais, Christopher John Marshall, Richard Wooster, Michael Rudolf Stratton, P. Andrew Futreal; Show fewer authors
Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; TIB-223
BCRC; 60474
CLS; 300155
IZSLER; BS TCL 94
Cell line databases/resources CLO; CLO_0003464
CLDB; cl1445
cancercelllines; CVCL_1229
Cell_Model_Passport; SIDM00853
Cosmic-CLP; 906999
DepMap; ACH-000835
IGRhCellID; GCT
LINCS_LDP; LCL-1752
Biological sample resources BioSample; SAMN03471887
BioSample; SAMN10987909
Chemistry resources ChEMBL-Cells; CHEMBL3308471
ChEMBL-Targets; CHEMBL1075448
GDSC; 906999
PharmacoDB; GCT_401_2019
PubChem_Cell_line; CVCL_1229
Encyclopedic resources Wikidata; Q54835514
Experimental variables resources EFO; EFO_0002182
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
FANTOM5_SSTAR; 10711-109H9
GEO; GSM184402
GEO; GSM184403
GEO; GSM827496
GEO; GSM887022
GEO; GSM888092
GEO; GSM1669807
Polymorphism and mutation databases Cosmic; 684227
Cosmic; 906999
IARC_TP53; 21341
LiGeA; CCLE_019
Progenetix; CVCL_1229
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number43