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Cellosaurus GAK (CVCL_1225)

[Text version]
Cell line name GAK
Accession CVCL_1225
Resource Identification Initiative To cite this cell line use: GAK (RRID:CVCL_1225)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Doubling time: 72 hours (PubMed=6431036); ~55 hours (Note=Lot 07202012) (JCRB=IFO50321); ~32 hours (Note=Lot 02242012) (JCRB=JCRB0180).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Inguinal lymph node; UBERON=UBERON_0001542.
Sequence variations
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (Cosmic-CLP=910932; DepMap=ACH-002120).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North77.89
East Asian, South22.1
South Asian0
European, North0
European, South0
Disease Vulvar melanoma (NCIt: C40329)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 86Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=910932; JCRB=IFO50321; JCRB=JCRB0180; PubMed=25877200

Markers:
AmelogeninX
CSF1PO10
D2S133818,20
D3S135816
D5S81810
D7S82011
D8S117910,11
D13S3179,13
D16S53912
D18S5113
D19S43314,15
D21S1129,30
FGA24
Penta D9,11
Penta E9,10
TH017
TPOX8,9
vWA15,18

Run an STR similarity search on this cell line
Publications

PubMed=6431036
Shiro Nozawa, Hiroaki Ohta, Katsumi Tsukazaki, Yasuhiro Udagawa, Soju Kurihara, Shojiro Kano;
A newly established melanoma cell line (GAK) with 5-S-cysteinyldopa phenotype.
Nihon Sanka Fujinka Gakkai Zasshi 36:883-892(1984)

PubMed=15467732; DOI=10.1038/sj.onc.1208152
Hideaki Tanami, Issei Imoto, Akira Hirasawa, Yasuhiro Yuki, Itaru Sonoda, Jun Inoue, Kohichiroh Yasui, Akiko Misawa-Furihata, Yutaka Kawakami, Johji Inazawa;
Involvement of overexpressed wild-type BRAF in the growth of malignant melanoma cell lines.
Oncogene 23:8796-8804(2004)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) JCRB; FDSC0034 - Discontinued
JCRB; IFO50321
JCRB; JCRB0180
Cell line databases/resources CLO; CLO_0037126
cancercelllines; CVCL_1225
Cell_Model_Passport; SIDM00543
CGH-DB; 9290-4
Cosmic-CLP; 910932
DepMap; ACH-002120
LINCS_LDP; LCL-1272
Biological sample resources BioSample; SAMN03472868
Chemistry resources ChEMBL-Cells; CHEMBL3308154
ChEMBL-Targets; CHEMBL2366301
GDSC; 910932
PharmacoDB; GAK_397_2019
PubChem_Cell_line; CVCL_1225
Encyclopedic resources Wikidata; Q54835424
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM1669803
Polymorphism and mutation databases Cosmic; 905204
Cosmic; 910932
Cosmic; 1995410
IARC_TP53; 21337
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number38