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Cellosaurus EW-3 (CVCL_1216)

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Cell line name EW-3
Synonyms EW3; IARC-EW3; IARC-EW-3; IARC-EW 3
Accession CVCL_1216
Resource Identification Initiative To cite this cell line use: EW-3 (RRID:CVCL_1216)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: Ewing Sarcoma Cell Line Atlas (ESCLA).
Population: Caucasian.
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; ChIP-seq; ERG.
Omics: Genomics; ChIP-seq; H3K27ac.
Omics: Genomics; ChIP-seq; H3K27me3.
Omics: Genomics; ChIP-seq; H3K4me3.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Genomics; Whole genome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; HGNC:3446; ERG + HGNC; HGNC:3508; EWSR1; Name(s)=EWSR1-ERG, EWS-ERG; Note=EWSR1 exon 7 with ERG exon 6 (PubMed=11423975; PubMed=16631476; PubMed=36476851).
  • Mutation; HGNC; HGNC:11355; STAG2; Simple; p.Arg216Ter (c.646C>T); Zygosity=Homozygous (PubMed=25223734; DepMap=ACH-002118).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; c.852_858del7; Zygosity=Unspecified (PubMed=19787792; PubMed=26428435).
  • Mutation; HGNC; HGNC:12363; TSC2; Simple; p.Arg462His (c.1385G>A); ClinVar=VCV000050193; Zygosity=Heterozygous (DepMap=ACH-002118).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0.33
East Asian, North0.83
East Asian, South0.4
South Asian6.18
European, North47.05
European, South45.2
Disease Ewing sarcoma (NCIt: C4817)
Ewing sarcoma (ORDO: Orphanet_319)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=949161; PubMed=19787792

Markers:
AmelogeninX,Y
CSF1PO11,12
D2S133820,22
D3S135816
D5S81812
D7S8208,11
D8S117912,14
D13S31712
D16S53911,12
D18S5116,18
D19S43313,15
D21S1129,31.2
FGA22,25
SE3315.3,16
TH019.3,10
TPOX8,9
vWA15,16

Run an STR similarity search on this cell line
Web pages Info; ESCLA; -; https://hgserver1.amc.nl/cgi-bin/r2/main.cgi?option=about_dscope
Publications

PubMed=6713356; DOI=10.1016/0165-4608(84)90002-5
Claude Turc-Carel, Irene Philip, Marie-Pierre Berger, Thierry Philip, Gilbert Marcel Lenoir;
Chromosome study of Ewing's sarcoma (ES) cell lines. Consistency of a reciprocal translocation t(11;22)(q24;q12).
Cancer Genet. Cytogenet. 12:1-19(1984)

PubMed=11423975; DOI=10.1038/sj.onc.1204437
Luce Dauphinot, Catherine De Oliveira, Thomas Melot, Nicolas Sevenet, Venetia Thomas, Bernard E. Weissman, Olivier Delattre;
Analysis of the expression of cell cycle regulators in Ewing cell lines: EWS-FLI-1 modulates p57KIP2and c-Myc expression.
Oncogene 20:3258-3265(2001)

PubMed=16631476; DOI=10.1016/j.cancergencyto.2005.11.006
Karoly Szuhai, Marije IJszenga, Hans J. Tanke, Carla Rosenberg, Pancras Cornelis Wilhelmus Hogendoorn;
Molecular cytogenetic characterization of four previously established and two newly established Ewing sarcoma cell lines.
Cancer Genet. Cytogenet. 166:173-179(2006)

PubMed=19787792; DOI=10.1002/gcc.20717
Laura Ottaviano, Karl-Ludwig Schaefer, Melanie Gajewski, Wolfgang Huckenbeck, Stefan E. Baldus, Uwe Rogel, Carlos Mackintosh, Enrique de Alava, Ola Myklebost, Stine Henrichson Kresse, Leonardo Andres Meza-Zepeda ...Show all 18 authors... , Massimo Serra, Anne-Marie Cleton-Jansen, Pancras Cornelis Wilhelmus Hogendoorn, Horst Buerger, Thomas Aigner, Helmut Erich Gabbert, Christopher Poremba; Show fewer authors
Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.
Genes Chromosomes Cancer 49:40-51(2010)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=21822310; DOI=10.1038/onc.2011.317
Carlos Mackintosh, Jose L. Ordonez, Daniel J. Garcia-Dominguez, Vicky Sevillano, Antonio Llombart-Bosch, Karoly Szuhai, Katia Scotlandi, Marco Alberghini, Raf Sciot, Friedl Sinnaeve, Pancras Cornelis Wilhelmus Hogendoorn ...Show all 17 authors... , Piero Picci, Sakari Knuutila, Uta Dirksen, Maria Debiec-Rychter, Karl-Ludwig Schaefer, Enrique de Alava; Show fewer authors
1q gain and CDT2 overexpression underlie an aggressive and highly proliferative form of Ewing sarcoma.
Oncogene 31:1287-1298(2012)

PubMed=25223734; DOI=10.1158/2159-8290.CD-14-0622; PMCID=PMC4264969
Franck Tirode, Didier Surdez, Xiao-Tu Ma, Matthew Parker, Marie-Cecile Le Deley, Armita Bahrami, Zhao-Jie Zhang, Eve Lapouble, Sandrine Grossetete-Lalami, Michael Rusch, Stephanie Reynaud ...Show all 35 authors... , Thomas Rio-Frio, Erin Hedlund, Gang Wu, Xiang Chen, Gaelle Pierron, Odile Oberlin, Sakina Zaidi, Gordon Lemmon, Pankaj Gupta, Bhavin Vadodaria, John Easton, Marta Gut, Li Ding, Elaine R. Mardis, Richard K. Wilson, Sheila Shurtleff, Valerie Laurence, Jean Michon, Perrine Marec-Berard, Ivo G. Gut, James R. Downing, Michael Anthony Dyer, Jing-Hui Zhang, Olivier Delattre; Show fewer authors
International Cancer Genome Consortium
St. Jude Children's Research Hospital-Washington University Pediatric Cancer Genome Project
Genomic landscape of Ewing sarcoma defines an aggressive subtype with co-association of STAG2 and TP53 mutations.
Cancer Discov. 4:1342-1353(2014)

PubMed=26428435; DOI=10.1016/j.ejca.2015.08.020
Laurens G.L. Sand, Katia Scotlandi, Dagmar Berghuis, B. Ewa Snaar-Jagalska, Piero Picci, Thomas Schmidt, Karoly Szuhai, Pancras Cornelis Wilhelmus Hogendoorn;
CXCL14, CXCR7 expression and CXCR4 splice variant ratio associate with survival and metastases in Ewing sarcoma patients.
Eur. J. Cancer 51:2624-2633(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

DOI=10.5282/edoc.27750
Martin Franz Orth;
Systematic multi-omics profiling of Ewing sarcoma cell lines.
Thesis PhD (2021); Ludwig Maximilians University of Munich; Munich; Germany

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

PubMed=36476851; DOI=10.1016/j.celrep.2022.111761; PMCID=PMC10333306
Martin Franz Orth, Didier Surdez, Tobias Faehling, Anna C. Ehlers, Aruna Marchetto, Sandrine Grossetete-Lalami, Richard Volckmann, Danny A. Zwijnenburg, Julia Sophie Gerke, Sakina Zaidi, Javier Alonso ...Show all 25 authors... , Ana Sastre, Sylvain Baulande, Martin Sill, Florencia Cidre-Aranaz, Shunya Ohmura, Thomas Kirchner, Stefanie M. Hauck, Eva Reischl, Melissa Gymrek, Stefan Michael Pfister, Konstantin Strauch, Jan Koster, Olivier Delattre, Thomas Georg Philipp Grunewald; Show fewer authors
Systematic multi-omics cell line profiling uncovers principles of Ewing sarcoma fusion oncogene-mediated gene regulation.
Cell Rep. 41:111761.1-111761.28(2022)

Cross-references
Cell line databases/resources CLO; CLO_0009872
cancercelllines; CVCL_1216
Cell_Model_Passport; SIDM00202
Cosmic-CLP; 949161
DepMap; ACH-002118
LINCS_LDP; LCL-1452
Chemistry resources ChEMBL-Cells; CHEMBL3308521
ChEMBL-Targets; CHEMBL2366307
GDSC; 949161
PharmacoDB; EW3_358_2019
PubChem_Cell_line; CVCL_1216
Encyclopedic resources Wikidata; Q54833002
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM510005
GEO; GSM1669789
GEO; GSM5359664
GEO; GSM5359665
GEO; GSM5359666
GEO; GSM5359667
GEO; GSM5359668
GEO; GSM5359669
GEO; GSM5362912
GEO; GSM5362913
GEO; GSM5362914
GEO; GSM5362915
GEO; GSM5362916
GEO; GSM5362917
GEO; GSM5363966
GEO; GSM5363967
GEO; GSM5363968
GEO; GSM5363969
GEO; GSM5363970
Polymorphism and mutation databases Cosmic; 684070
Cosmic; 949161
Cosmic; 1995405
Cosmic; 2250460
IARC_TP53; 27038
IARC_TP53; 27169
Progenetix; CVCL_1216
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number37