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Cellosaurus D-247MG (CVCL_1153)

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Cell line name D-247MG
Synonyms D-247 MG; D-247-MG; D247MG
Accession CVCL_1153
Resource Identification Initiative To cite this cell line use: D-247MG (RRID:CVCL_1153)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 30.17 hours (PubMed=2881426); 42 hours (PubMed=9842975).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Misspelling: D24.7; PubMed=11351043.
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (PubMed=9842975).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; c.210-1G>C; ClinVar=VCV000428260; Zygosity=Homozygous; Note=Splice acceptor mutation (Cosmic-CLP=946367; DepMap=ACH-002224).
  • Mutation; HGNC; HGNC:11998; TP53; None_reported; -; Zygosity=- (PubMed=14614447).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0.5
East Asian, North0
East Asian, South0
South Asian1.46
European, North67.83
European, South30.2
Disease Gliosarcoma (NCIt: C3796)
Gliosarcoma (ORDO: Orphanet_251576)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 80Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=946367; PubMed=22570425

Markers:
AmelogeninX
CSF1PO9,11
D3S135817,18
D5S81810,12
D7S8209,13
D8S117915
D13S3178,10
D16S5399,12
D18S5115,17
D21S1130
FGA24,27
Penta D11,12
Penta E13,18
TH016,9
TPOX9,11
vWA17,18

Run an STR similarity search on this cell line
Publications

PubMed=2881426; DOI=10.1007/BF00687952
Sandra H. Bigner, Henry Seth Friedman, Jaclyn A. Biegel, Carol J. Wikstrand, Joachim Mark, Rolf Gebhardt, Lawrence F. Eng, Darell Doty Bigner;
Specific chromosomal abnormalities characterize four established cell lines derived from malignant human gliomas.
Acta Neuropathol. 72:86-97(1986)

PubMed=3675803
Sandra H. Bigner, Rolf Bjerkvig, Ole Didrik Laerum, Laurence H. Muhlbaier, Darell Doty Bigner;
DNA content and chromosomes in permanent cultured cell lines derived from malignant human gliomas.
Anal. Quant. Cytol. Histol. 9:435-444(1987)

PubMed=9220028; DOI=10.1016/S0303-7207(97)00080-4
Taraneh R. Sharif, Wei Luo, Mohammed Sharif;
Functional expression of bombesin receptor in most adult and pediatric human glioblastoma cell lines; role in mitogenesis and in stimulating the mitogen-activated protein kinase pathway.
Mol. Cell. Endocrinol. 130:119-130(1997)

PubMed=9842975; DOI=10.1002/(SICI)1097-0215(19981218)79:6<640::AID-IJC15>3.0.CO;2-Z
Michael Weller, Johannes Rieger, Cornelia Grimmel, Erwin G. Van Meir, Nicolas De Tribolet, Stanislaw Krajewski, John C. Reed, Andreas von Deimling, Johannes Dichgans;
Predicting chemoresistance in human malignant glioma cells: the role of molecular genetic analyses.
Int. J. Cancer 79:640-644(1998)

DOI=10.1007/0-306-46861-1_11
Francis Ali-Osman;
Brain tumors.
(In book chapter) Human cell culture. Vol. 2. Cancer cell lines part 2; Masters, John R.W. & Palsson, Bernhard Orn (eds.); pp.167-184; Kluwer Academic Publishers; New York; USA (1999)

PubMed=10402232; DOI=10.3892/ijo.15.2.237
Taraneh R. Sharif, Mohammed Sharif;
Overexpression of protein kinase C epsilon in astroglial brain tumor derived cell lines and primary tumor samples.
Int. J. Oncol. 15:237-243(1999)

PubMed=10416987; DOI=10.1111/j.1750-3639.1999.tb00536.x; PMCID=PMC8098486
Nobuaki Ishii, Daniel Maier, Adrian Merlo, Mitsuhiro Tada, Yutaka Sawamura, Annie-Claire Diserens, Erwin G. Van Meir;
Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines.
Brain Pathol. 9:469-479(1999)

PubMed=11351043; DOI=10.1038/labinvest.3780280
Angela Bik-Yu Hui, Kwok-Wai Lo, Xiao-Lu Yin, Wai-Sang Poon, Ho-Keung Ng;
Detection of multiple gene amplifications in glioblastoma multiforme using array-based comparative genomic hybridization.
Lab. Invest. 81:717-723(2001)

PubMed=14614447; DOI=10.1038/sj.onc.1207198
Jorg Wischhusen, Ulrike Naumann, Hiroko Ohgaki, Farzan Rastinejad, Michael Weller;
CP-31398, a novel p53-stabilizing agent, induces p53-dependent and p53-independent glioma cell death.
Oncogene 22:8233-8245(2003)

PubMed=14655754; DOI=10.1111/j.1750-3639.2003.tb00479.x; PMCID=PMC8095903
Oliver Bahr, Johannes Rieger, Frank Duffner, Richard Meyermann, Michael Weller, Wolfgang Wick;
P-glycoprotein and multidrug resistance-associated protein mediate specific patterns of multidrug resistance in malignant glioma cell lines, but not in primary glioma cells.
Brain Pathol. 13:482-494(2003)

PubMed=17595512; DOI=10.1159/000104150
Johannes Rieger, Brigitte Frank, Michael Weller, Wolfgang Wick;
Mechanisms of resistance of human glioma cells to Apo2 ligand/TNF-related apoptosis-inducing ligand.
Cell. Physiol. Biochem. 20:23-34(2007)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=22570425; DOI=10.1093/neuonc/nos072; PMCID=PMC3367844
Pierre Bady, Annie-Claire Diserens, Vincent Castella, Stefanie Kalt, Karl Heinimann, Marie-France Hamou, Mauro Delorenzi, Monika E. Hegi;
DNA fingerprinting of glioma cell lines and considerations on similarity measurements.
Neuro-oncol. 14:701-711(2012)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line databases/resources CLO; CLO_0009883
cancercelllines; CVCL_1153
Cell_Model_Passport; SIDM00622
Cosmic-CLP; 946367
DepMap; ACH-002224
LINCS_LDP; LCL-1370
Chemistry resources ChEMBL-Cells; CHEMBL3308542
ChEMBL-Targets; CHEMBL2366237
GDSC; 946367
PharmacoDB; D247MG_271_2019
PubChem_Cell_line; CVCL_1153
Encyclopedic resources Wikidata; Q54817124
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM1669715
Polymorphism and mutation databases Cosmic; 849881
Cosmic; 946367
Cosmic; 2367513
IARC_TP53; 23118
IARC_TP53; 27689
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number37