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Cellosaurus A-253 (CVCL_1060)

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Cell line name A-253
Synonyms A 253; A253
Accession CVCL_1060
Resource Identification Initiative To cite this cell line use: A-253 (RRID:CVCL_1060)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Population: Caucasian.
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Salivary gland, submandibular gland; UBERON=UBERON_0001736.
Sequence variations
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Ala17fs*5 (c.49_61delGCCACGGCCGCGG); Zygosity=Heterozygous (ATCC=HTB-41; Cosmic-CLP=906794; DepMap=ACH-000740).
  • Mutation; HGNC; HGNC:7133; KMT2D; Simple; p.Gly3092Ter (c.9274G>T); Zygosity=Heterozygous (Cosmic-CLP=906794; DepMap=ACH-000740).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Leu152Pro (c.455T>C); ClinVar=VCV000536551; Zygosity=Homozygous (Cosmic-CLP=906794; DepMap=ACH-000740).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Glu180fs*67 (c.539delA); Zygosity=Homozygous (ATCC=HTB-41; Cosmic-CLP=906794; DepMap=ACH-000740).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*32:01,32:01
HLA-BB*44:02,44:02
HLA-CC*03:04,05:01
Class II
HLA-DQDQB1*06:08,06:08
HLA-DRDRB1*13:10,13:10
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.97
Native American0
East Asian, North1.81
East Asian, South0
South Asian0
European, North61.82
European, South35.39
Disease Submandibular gland squamous cell carcinoma (NCIt: C5943)
Squamous cell carcinoma of salivary glands (ORDO: Orphanet_500481)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_A2EP (A-253 clone B2)CVCL_A2EQ (A-253 clone B4)CVCL_A2ER (A-253 clone B9)
CVCL_A2ES (A-253 clone D3)CVCL_A2ET (A-253 clone F11)
Sex of cell Male
Age at sampling 54Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-41; Cosmic-CLP=906794; IZSLER=BS TCL 199; PubMed=29923277

Markers:
AmelogeninX
CSF1PO12,13
D2S133825,26
D3S135815
D5S81811
D7S82010,12
D8S117913
D13S3179,12
D16S53912
D18S5113
D19S43313
D21S1130,30.2
FGA25
Penta D13
Penta E7
TH016,8
TPOX8,12
vWA16

Run an STR similarity search on this cell line
Publications

PubMed=4357758; DOI=10.1093/jnci/51.5.1417
Donald J. Giard, Stuart A. Aaronson, George J. Todaro, Paul Arnstein, John H. Kersey, Harvey Dosik, Wade P. Parks;
In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.
J. Natl. Cancer Inst. 51:1417-1423(1973)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Jorgen Fogh, Jens M. Fogh, Thomas Orfeo;
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Jorgen Fogh, William C. Wright, James D. Loveless;
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=6256643; DOI=10.1038/288724a0
Rufus S. Day 3rd, Chuck H.J. Ziolkowski, Dominic A. Scudiero, Sharon A. Meyer, Anthony S. Lubiniecki, Anthony Joseph Girardi, Sheila Margaret Galloway, Gaither D. Bynum;
Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains.
Nature 288:724-727(1980)

PubMed=22282976; DOI=10.1093/carcin/1.1.21
Rufus S. Day 3rd, Chuck H.J. Ziolkowski, Dominic A. Scudiero, Sharon A. Meyer, Michael R. Mattern;
Human tumor cell strains defective in the repair of alkylation damage.
Carcinogenesis 1:21-32(1980)

PubMed=3518877; DOI=10.3109/07357908609038260
Jorgen Fogh;
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3138611; DOI=10.1177/019459988809800603
Thomas P.U. Wustrow, Alexander Raffael, Gunter K. Valet;
Multiparametric flow cytometry of human squamous cell carcinoma lines from the head and neck.
Otolaryngol. Head Neck Surg. 98:552-557(1988)

DOI=10.1016/B978-0-12-333530-2.50007-1
Thomas E. Carey;
Head and neck tumor cell lines.
(In book chapter) Atlas of human tumor cell lines; Hay, Robert J. & Park, Jae-Gahb & Gazdar, Adi F. (eds.); pp.79-120; Academic Press; New York; USA (1994)

PubMed=20014447; DOI=10.1002/hed.21311; PMCID=PMC2930055
Ivy F.L. Tsui, Cathie Garnis;
Integrative molecular characterization of head and neck cancer cell model genomes.
Head Neck 32:1143-1160(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=29923277; DOI=10.1111/odi.12920
Li-Chieh Lin, Osama Elkashty, Murali Ramamoorthi, Nathalie Trinh, You-Nan Liu, Gulshan Sunavala-Dossabhoy, Thomas John F. Pranzatelli, Drew G. Michael, Clara Chivasso, Jason Perret, John A. Chiorini ...Show all 13 authors... , Christine Delporte, Simon D. Tran; Show fewer authors
Cross-contamination of the human salivary gland HSG cell line with HeLa cells: a STR analysis study.
Oral Dis. 24:1477-1483(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-7902 - Discontinued
ATCC; HTB-41
BCRC; 60367
BCRJ; 0322
IZSLER; BS TCL 199
KCLB; 30041 - Discontinued
Cell line databases/resources CLO; CLO_0001569
CLO; CLO_0001570
cancercelllines; CVCL_1060
Cell_Model_Passport; SIDM00796
Cosmic-CLP; 906794
DepMap; ACH-000740
LINCS_LDP; LCL-2034
TOKU-E; 462
Anatomy/cell type resources BTO; BTO_0003206
Biological sample resources BioSample; SAMN03471117
BioSample; SAMN10988034
CRISP screens repositories BioGRID_ORCS_Cell_line; 824
Chemistry resources ChEMBL-Cells; CHEMBL3307893
ChEMBL-Targets; CHEMBL614255
GDSC; 906794
PharmacoDB; A253_39_2019
PubChem_Cell_line; CVCL_1060
Encyclopedic resources Wikidata; Q54605985
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM422867
GEO; GSM423670
GEO; GSM886852
GEO; GSM887917
GEO; GSM1669580
Polymorphism and mutation databases Cosmic; 688036
Cosmic; 906794
Cosmic; 921831
Cosmic; 1006471
Cosmic; 1017790
Cosmic; 1041671
Cosmic; 1995330
Cosmic; 2646649
IARC_TP53; 21160
LiGeA; CCLE_437
Progenetix; CVCL_1060
Proteomic databases PRIDE; PXD002486
PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number43