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Cellosaurus HK-2 [Human kidney] (CVCL_0302)

[Text version]
Cell line name HK-2 [Human kidney]
Synonyms Hk-2; HK2; Human Kidney-2
Accession CVCL_0302
Resource Identification Initiative To cite this cell line use: HK-2 [Human kidney] (RRID:CVCL_0302)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Caucasian.
Transfected with: UniProtKB; P03126; Human papillomavirus type 16 protein E6.
Transfected with: UniProtKB; P03129; Human papillomavirus type 16 protein E7.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
Omics: SNP array analysis.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0
East Asian, North3.68
East Asian, South0
South Asian0
European, North62.77
European, South29.92
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_UC05 (HK-2-KIM-1)CVCL_C9F4 (HK2/Cas9-hyg)CVCL_WZ51 (HKC)
Sex of cell Male
Age at sampling Adult
Category Transformed cell line
STR profile Source(s): AddexBio=T0011004/4929; ATCC=CRL-2190; CCRID; KCLB=22190

AmelogeninX (CCRID)
X,Y (AddexBio=T0011004/4929; ATCC=CRL-2190; KCLB=22190)
Penta D9,12
Penta E10,11

Run an STR similarity search on this cell line

PubMed=8127021; DOI=10.1038/ki.1994.6
Ryan M.J., Johnson G., Kirk J., Fuerstenberg S.M., Zager R.A., Torok-Storb B.
HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney.
Kidney Int. 45:48-57(1994)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20555413; DOI=10.1139/y10-023
Gunness P., Aleksa K., Kosuge K., Ito S., Koren G.
Comparison of the novel HK-2 human renal proximal tubular cell line with the standard LLC-PK1 cell line in studying drug-induced nephrotoxicity.
Can. J. Physiol. Pharmacol. 88:448-455(2010)

PubMed=22949125; DOI=10.1002/ijc.27822
Pawlowski R., Muhl S.M., Sulser T., Krek W., Moch H., Schraml P.
Loss of PBRM1 expression is associated with renal cell carcinoma progression.
Int. J. Cancer 132:E11-E17(2013)

PubMed=28489074; DOI=10.1038/ncomms15165
Sinha R., Winer A.G., Chevinsky M., Jakubowski C., Chen Y.-B., Dong Y.-Y., Tickoo S.K., Reuter V.E., Russo P., Coleman J.A., Sander C., Hsieh J.J.-D., Hakimi A.A.
Analysis of renal cancer cell lines from two major resources enables genomics-guided cell line selection.
Nat. Commun. 8:15165.1-15165.10(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=33122286; DOI=10.1681/ASN.2020010009
Khundmiri S.J., Chen L.-H., Lederer E.D., Yang C.-R., Knepper M.A.
Transcriptomes of major proximal tubule cell culture models.
J. Am. Soc. Nephrol. 32:86-97(2021)

Cell line collections (Providers) AddexBio; T0011004/4929
ATCC; CRL-2190
BCRC; 60097
CLS; 305021
KCB; KCB 200815YJ
KCLB; 22190
Cell line databases/resources CLO; CLO_0003771
MCCL; MCC:0000193
cancercelllines; CVCL_0302
Cell_Model_Passport; SIDM01818
DepMap; ACH-001087
Lonza; 769
Anatomy/cell type resources BTO; BTO:0003575
Biological sample resources BioSample; SAMN03472562
CRISP screens repositories BioGRID_ORCS_Cell_line; 1711
Chemistry resources ChEMBL-Cells; CHEMBL4295396
ChEMBL-Targets; CHEMBL4296428
PharmacoDB; HK2_545_2019
PubChem_Cell_line; CVCL_0302
Encyclopedic resources Wikidata; Q54889732
Gene expression databases GEO; GSM827261
GEO; GSM4009069
GEO; GSM4009073
Polymorphism and mutation databases Cosmic; 2520628
Progenetix; CVCL_0302
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number31