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Cellosaurus HK-2 [Human kidney] (CVCL_0302)

[Text version]
Cell line name HK-2 [Human kidney]
Synonyms Hk-2; HK2; Human Kidney-2
Accession CVCL_0302
Resource Identification Initiative To cite this cell line use: HK-2 [Human kidney] (RRID:CVCL_0302)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Caucasian.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03126; Human papillomavirus type 16 protein E6.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03129; Human papillomavirus type 16 protein E7.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Kidney, proximal tubule; UBERON=UBERON_0004134.
Cell type: Epithelial cell of proximal tubule; CL=CL_0002306.
Genome ancestry Source: PubMed=30894373

Origin% genome
African3.63
Native American0
East Asian, North3.68
East Asian, South0
South Asian0
European, North62.77
European, South29.92
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_UC05 (HK-2-KIM-1)CVCL_C9F4 (HK2/Cas9-hyg)CVCL_WZ51 (HKC)
CVCL_E1LA (HyCyte HK-2 KO-hKDM5A)CVCL_D6BQ (HyCyte HK-2 KO-hNPHP3)CVCL_D9W9 (Ubigene HK-2 ALDOB KO)
Sex of cell Male
Age at sampling Adult
Category Transformed cell line
STR profile Source(s): AddexBio=T0011004/4929; ATCC=CRL-2190; CCRID; KCLB=22190

Markers:
AmelogeninX (CCRID)
X,Y (AddexBio=T0011004/4929; ATCC=CRL-2190; KCLB=22190)
CSF1PO13
D2S133817,25
D3S135816,17
D5S81812
D7S82010,11
D8S117910,14
D13S3179
D16S53911,12
D18S5112
D19S43315,15.2
D21S1128,30
FGA20,22
Penta D9,12
Penta E10,11
TH019
TPOX8,9
vWA17,18

Run an STR similarity search on this cell line
Publications

PubMed=8127021; DOI=10.1038/ki.1994.6
Michael J. Ryan, Gretchen Johnson, Judy Kirk, Sally M. Fuerstenberg, Richard A. Zager, Beverly Torok-Storb;
HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney.
Kidney Int. 45:48-57(1994)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20555413; DOI=10.1139/y10-023
Patricia Gunness, Katarina Aleksa, Kazuhiro Kosuge, Shinya Ito, Gideon Koren;
Comparison of the novel HK-2 human renal proximal tubular cell line with the standard LLC-PK1 cell line in studying drug-induced nephrotoxicity.
Can. J. Physiol. Pharmacol. 88:448-455(2010)

PubMed=22949125; DOI=10.1002/ijc.27822
Rafal Pawlowski, Sarah M. Muhl, Tullio Sulser, Wilhelm Krek, Holger Moch, Peter Schraml;
Loss of PBRM1 expression is associated with renal cell carcinoma progression.
Int. J. Cancer 132:E11-E17(2013)

PubMed=28489074; DOI=10.1038/ncomms15165; PMCID=PMC5436135
Rileen Sinha, Andrew G. Winer, Michael Stephen Chevinsky, Christopher Jakubowski, Ying-Bei Chen, Yi-Yu Dong, Satish K. Tickoo, Victor E. Reuter, Paul Russo, Jonathan A. Coleman, Chris Sander ...Show all 13 authors... , James J.-D. Hsieh, Abraham Ari Hakimi; Show fewer authors
Analysis of renal cancer cell lines from two major resources enables genomics-guided cell line selection.
Nat. Commun. 8:15165.1-15165.10(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=33122286; DOI=10.1681/ASN.2020010009; PMCID=PMC7894662
Syed J. Khundmiri, Li-He Chen, Eleanor D. Lederer, Chin-Rang Yang, Mark A. Knepper;
Transcriptomes of major proximal tubule cell culture models.
J. Am. Soc. Nephrol. 32:86-97(2021)

Cross-references
Cell line collections (Providers) AddexBio; T0011004/4929
ATCC; CRL-2190
BCRC; 60097
CCTCC; GDC0152
CLS; 305021
KCB; KCB 200815YJ
KCLB; 22190
Ubigene; YC-C140
Cell line databases/resources CLO; CLO_0003771
MCCL; MCC:0000193
cancercelllines; CVCL_0302
CCRID; 3101HUMSCSP511
CCRID; 4201HUM-CCTCC00152
Cell_Model_Passport; SIDM01818
DepMap; ACH-001087
Lonza; 769
Anatomy/cell type resources BTO; BTO_0003575
Biological sample resources BioSample; SAMN03472562
ENCODE; ENCBS188NWH
CRISP screens repositories BioGRID_ORCS_Cell_line; 1711
Chemistry resources ChEMBL-Cells; CHEMBL4295396
ChEMBL-Targets; CHEMBL4296428
PharmacoDB; HK2_545_2019
PubChem_Cell_line; CVCL_0302
Encyclopedic resources Wikidata; Q54889732
Experimental variables resources EFO; EFO_0022529
Gene expression databases GEO; GSM827261
GEO; GSM4009069
GEO; GSM4009073
Polymorphism and mutation databases Cosmic; 2520628
Progenetix; CVCL_0302
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number35