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Cellosaurus CMK (CVCL_0216)

[Text version]
Cell line name CMK
Accession CVCL_0216
Resource Identification Initiative To cite this cell line use: CMK (RRID:CVCL_0216)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: ENCODE project common cell types; tier 3.
Part of: LL-100 blood cancer cell line panel.
Population: Japanese.
Doubling time: ~40 hours (PubMed=2527057); ~40-50 hours (DSMZ=ACC-392).
Microsatellite instability: Stable (MSS) (PubMed=10739008; PubMed=11226526; Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene fusion; HGNC; HGNC:4024; FXR2 + HGNC; HGNC:11998; TP53; Name(s)=TP53-FXR2 (from child cell line CMK-86).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Asp49His (c.145G>C); ClinVar=VCV000135948; Zygosity=Heterozygous (Cosmic-CLP=910566; DepMap=ACH-000641).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*02:01,24:02
HLA-BB*15:18,40:02
HLA-CC*08:01,08:01

Source: DSMZCellDive=ACC-392
Class I
HLA-AA*02:01:01,24:02:01
HLA-BB*15:18:01,40:02:01
HLA-CC*03:04:01,08:01:01
Class II
HLA-DRDRA*01:01:01,01:01:01
DRB1*11:01:01,11:01:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.58
Native American0
East Asian, North93.95
East Asian, South3.14
South Asian0
European, North0
European, South2.33
Disease Down syndrome (NCIt: C2993)
Myeloid leukemia associated with Down syndrome (NCIt: C43223)
Down syndrome (ORDO: Orphanet_870)
Acute megakaryoblastic leukemia in Down syndrome (ORDO: Orphanet_99887)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_RM24 (CMK-6)CVCL_2804 (CMK-86)
Sex of cell Male
Age at sampling 10M
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=910566; DSMZ=ACC-392

Markers:
AmelogeninX,Y
CSF1PO11,13
D2S133820,23
D3S135816,18
D5S81811,12
D7S82011,12
D8S117910,11
D13S31711
D16S5399,11
D18S5113,15
D19S43313,15
D21S1129,30
FGA21,22
Penta D9,10
Penta E11,12
TH017
TPOX9,11
vWA18

Run an STR similarity search on this cell line
Web pages Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/human/CMK_Stam_protocol.pdf
Publications

PubMed=2527057; DOI=10.1111/j.1365-2141.1989.tb07681.x
Takeyuki Sato, Akira Fuse, Mitsuoki Eguchi, Yasuhide Hayashi, Ryukichi Ryo, Masashi Adachi, Yuji Kishimoto, Masanao Teramura, Hideaki Mizoguchi, Yukichi Shima, Isao Komori ...Show all 14 authors... , Shousuke Sunami, Yuri Okimoto, Hironori Nakajima; Show fewer authors
Establishment of a human leukaemic cell line (CMK) with megakaryocytic characteristics from a Down's syndrome patient with acute megakaryoblastic leukaemia.
Br. J. Haematol. 72:184-190(1989)

PubMed=2665839; DOI=10.1182/blood.V74.1.42.42
Norio Komatsu, Toshio Suda, Masaaki Moroi, Naomi Tokuyama, Yoichi Sakata, Masayuki Okada, Tsutomu Nishida, Yoshikatsu Hirai, Takeyuki Sato, Akira Fuse, Yasusada Miura;
Growth and differentiation of a human megakaryoblastic cell line, CMK.
Blood 74:42-48(1989)

PubMed=2217443
Yasusada Miura, Norio Komatsu, Toshio Suda;
Growth and differentiation of two human megakaryoblastic cell lines CMK and UT-7.
Prog. Clin. Biol. Res. 356:259-270(1990)

PubMed=1991165; DOI=10.1182/blood.V77.3.481.481
Mamoru Sakaguchi, Takeyuki Sato, Jerome Elliot Groopman;
Human immunodeficiency virus infection of megakaryocytic cells.
Blood 77:481-485(1991)

PubMed=1571549; DOI=10.1182/blood.V79.9.2378.2378
Koichi Sugimoto, Hideo Toyoshima, Ryuichi Sakai, Kiyoshi Miyagawa, Koichi Hagiwara, Fuyuki Ishikawa, Fumimaro Takaku, Yoshio Yazaki, Hisamaru Hirai;
Frequent mutations in the p53 gene in human myeloid leukemia cell lines.
Blood 79:2378-2383(1992)

PubMed=8784480; DOI=10.1159/000227600
Akinori Shinohara, Eiji Shimizu, Minoru Takada, Saburo Sone;
Lack of c-mpl proto-oncogene transcripts and growth-stimulatory effects of thrombopoietin on human small cell lung cancer cell lines.
Oncology 53:426-434(1996)

PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x; PMCID=PMC5921588
Noriko Hosoya, Akira Hangaishi, Seishi Ogawa, Kiyoshi Miyagawa, Kinuko Mitani, Yoshio Yazaki, Hisamaru Hirai;
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Jpn. J. Cancer Res. 89:33-39(1998)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x; PMCID=PMC5921886
Jun Takizawa, Ritsuro Suzuki, Hiroyuki Kuroda, Atae Utsunomiya, Yoshitoyo Kagami, Tatsuroh Joh, Yoshifusa Aizawa, Ryuzo Ueda, Masao Seto;
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=10674912; DOI=10.3109/10428190009148861
Takeyuki Sato, Hiromasa Sekine, Harumi Kakuda, Nobuyuki Miura, Masataka Sunohara, Akira Fuse;
HIV infection of megakaryocytic cell lines.
Leuk. Lymphoma 36:397-404(2000)

PubMed=10739008; DOI=10.1016/S0145-2126(99)00182-4
Kaoru Inoue, Takashi Kohno, Satoshi Takakura, Yasuhide Hayashi, Hideaki Mizoguchi, Jun Yokota;
Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.
Leuk. Res. 24:255-262(2000)

DOI=10.1016/B978-0-12-221970-2.50457-5
Hans Gunther Drexler;
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=11226526; DOI=10.1016/S0145-2126(00)00121-1
Kaoru Inoue, Takashi Kohno, Satoshi Takakura, Yasuhide Hayashi, Hideaki Mizoguchi, Jun Yokota;
Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000), 255-262.
Leuk. Res. 25:275-278(2001)

PubMed=15843827; DOI=10.1038/sj.leu.2403749
Anna K. Hagstrom-Andersson, Patrik Eden, David Lindgren, Jens Nilsson, Carin Lassen, Jesper Heldrup, Magnus Fontes, Ake Borg, Felix Mitelman, Bertil Johansson, Mattias Hoglund, Thoas Fioretos;
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Leukemia 19:1042-1050(2005)

PubMed=16408098; DOI=10.1038/sj.leu.2404081
Hilmar Quentmeier, Roderick A.F. MacLeod, Margarete Zaborski, Hans Gunther Drexler;
JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Leukemia 20:471-476(2006)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23325432; DOI=10.1101/gr.147942.112; PMCID=PMC3589544
Katherine E. Varley, Jason Gertz, Kevin M. Bowling, Stephanie L. Parker, Timothy E. Reddy, Florencia Pauli-Behn, Marie K. Cross, Brian A. Williams, John A. Stamatoyannopoulos, Gregory E. Crawford, Devin Michael Absher ...Show all 13 authors... , Barbara Jane Wold, Richard M. Myers; Show fewer authors
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=23955599; DOI=10.1038/ng.2731
Ayana Kon, Lee-Yung Shih, Masashi Minamino, Masashi Sanada, Yuichi Shiraishi, Yasunobu Nagata, Ken-ichi Yoshida, Yusuke Okuno, Masashige Bando, Ryuichiro Nakato, Shumpei Ishikawa ...Show all 41 authors... , Aiko Sato-Otsubo, Genta Nagae, Aiko Nishimoto, Claudia Haferlach, Daniel Nowak, Yusuke Sato, Tamara Alpermann, Masao Nagasaki, Teppei Shimamura, Hiroko Tanaka, Kenichi Chiba, Ryo Yamamoto, Tomoyuki Yamaguchi, Makoto Otsu, Naoshi Obara, Mamiko Sakata-Yanagimoto, Tsuyoshi Nakamaki, Ken Ishiyama, Florian Nolte, Wolf-Karsten Hofmann, Shuichi Miyawaki, Shigeru Chiba, Hiraku Mori, Hiromitsu Nakauchi, Harold Phillip Koeffler, Hiroyuki Aburatani, Torsten Haferlach, Katsuhiko Shirahige, Satoru Miyano, Seishi Ogawa; Show fewer authors
Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.
Nat. Genet. 45:1232-1237(2013)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5; PMCID=PMC6167786
Kar-Tong Tan, Ling-Wen Ding, Qiao-Yang Sun, Zhen-Tang Lao, Wenwen Chien, Xi Ren, Jin-Fen Xiao, Xin-Yi Loh, Liang Xu, Michael Lill, Anand Mayakonda ...Show all 14 authors... , De-Chen Lin, Henry He Yang, Harold Phillip Koeffler; Show fewer authors
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940.1-940.13(2018)

PubMed=30629668; DOI=10.1371/journal.pone.0210404; PMCID=PMC6328144
Cord C. Uphoff, Claudia Pommerenke, Sabine A. Denkmann, Hans Gunther Drexler;
Screening human cell lines for viral infections applying RNA-Seq data analysis.
PLoS ONE 14:e0210404.1-e0210404.22(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31160637; DOI=10.1038/s41598-019-44491-x; PMCID=PMC6547646
Hilmar Quentmeier, Claudia Pommerenke, Wilhelm Gerhard Dirks, Sonja Eberth, Max Koeppel, Roderick A.F. MacLeod, Stefan Nagel, Klaus G. Steube, Cord C. Uphoff, Hans Gunther Drexler;
The LL-100 panel: 100 cell lines for blood cancer studies.
Sci. Rep. 9:8218.1-8218.14(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
David Paul Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald 3rd, Marian Kalocsay, Judit Jane-Valbuena, Ellen T. Gelfand, Devin K. Schweppe, Mark P. Jedrychowski, Javad Golji ...Show all 20 authors... , Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi Alexander Garraway, William Raj Sellers, Steven P. Gygi; Show fewer authors
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=36982453; DOI=10.3390/ijms24065377; PMCID=PMC10049680
Rafal Skopek, Malgorzata Palusinska, Katarzyna Kaczor-Keller, Rafal Pingwara, Anna Papierniak-Wygladala, Tino Schenk, Slawomir Lewicki, Artur Zelent, Lukasz Szymanski;
Choosing the right cell line for acute myeloid leukemia (AML) research.
Int. J. Mol. Sci. 24:5377.1-5377.34(2023)

Cross-references
Cell line collections (Providers) DSMZ; ACC-392
KCB; KCB 2017032YJ
Cell line databases/resources CLO; CLO_0002526
MCCL; MCC:0000105
cancercelllines; CVCL_0216
CCRID; 5301HUM-KCB17032YJ
Cell_Model_Passport; SIDM00959
Cosmic-CLP; 910566
DepMap; ACH-000641
DSMZCellDive; ACC-392
LINCS_LDP; LCL-1051
TOKU-E; 4113
Anatomy/cell type resources BTO; BTO_0000978
Biological sample resources BioSample; SAMN03473574
BioSample; SAMN10988101
ENCODE; ENCBS071ENC
Chemistry resources ChEMBL-Cells; CHEMBL3308149
ChEMBL-Targets; CHEMBL2366177
GDSC; 910566
PharmacoDB; CMK_210_2019
PubChem_Cell_line; CVCL_0216
Encyclopedic resources Wikidata; Q54813770
Experimental variables resources EFO; EFO_0003037
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
ArrayExpress; E-MTAB-7721
ArrayExpress; E-MTAB-7722
GEO; GSM236781
GEO; GSM236817
GEO; GSM510583
GEO; GSM886938
GEO; GSM888005
GEO; GSM1374449
Polymorphism and mutation databases Cosmic; 787423
Cosmic; 910566
Cosmic; 919131
Cosmic; 975241
Cosmic; 1012061
Cosmic; 1070695
Cosmic; 1176598
Cosmic; 1191081
Cosmic; 1191702
Cosmic; 1281307
Cosmic; 1286955
Cosmic; 1315742
Cosmic; 1524829
Cosmic; 1559970
Cosmic; 2089668
Cosmic; 2131535
Cosmic; 2306202
IARC_TP53; 21228
LiGeA; CCLE_184
Progenetix; CVCL_0216
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number45