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Cellosaurus bEnd.3 (CVCL_0170)

[Text version]
Cell line name bEnd.3
Synonyms bEND.3; b.End3; Bend.3; bEnd3; BEND-3; BEND3; brain-derived Endothelial cells.3
Accession CVCL_0170
Resource Identification Initiative To cite this cell line use: bEnd.3 (RRID:CVCL_0170)
Comments Characteristics: Widely used for blood vascular research.
Doubling time: 26-30 hours (PubMed=14568334).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03077; Murine polyomavirus (strain A2) (MPyV) middle T antigen (PyMT).
Transformant: NCBI_TaxID; 10636; Murine polyomavirus (strain A2) (MPyV).
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Cell type: Endothelial cell; CL=CL_0000115.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Children:
CVCL_E1JC (HyCyte bEnd.3 KO-mCdh5)
Sex of cell Sex unspecified
Age at sampling 6W
Category Transformed cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-crl-2299
Info; Wikipedia; -; https://en.wikipedia.org/wiki/BEnd.3
Publications

PubMed=2379237; DOI=10.1016/0092-8674(90)90009-4
Roberto Montesano, Michael Sean Pepper, Uta Mohle-Steinlein, Werner Risau, Erwin Friedrich Wagner, Lelio Orci;
Increased proteolytic activity is responsible for the aberrant morphogenetic behavior of endothelial cells expressing the middle T oncogene.
Cell 62:435-445(1990)

PubMed=14568334; DOI=10.1016/S0006-8993(03)03443-7
Yadollah Omidi, Lee Campbell, Jaleh Barar, David Connell, Saeed Akhtar, Mark Gumbleton;
Evaluation of the immortalised mouse brain capillary endothelial cell line, b.End3, as an in vitro blood-brain barrier model for drug uptake and transport studies.
Brain Res. 990:95-112(2003)

PubMed=22224974; DOI=10.1089/scd.2011.0501
Katherine Joubin, Amelia Richardson, Natalia Novoa, Edmund Tu, Mark J. Tomishima;
The endothelial cell line bEnd.3 maintains human pluripotent stem cells.
Stem Cells Dev. 21:2312-2321(2012)

PubMed=25282873; PMCID=PMC4636729
David Yuen, Rose Kristine Leu, Julie Tse, S. Wang, L.L. Chen, Lu Chen;
Novel characterization of bEnd.3 cells that express lymphatic vessel endothelial hyaluronan receptor-1.
Lymphology 47:73-81(2014)

Cross-references
Cell line collections (Providers) AddexBio; C0005030/5019
ATCC; CRL-2299
BCRC; 60515
ECACC; 96091929
JCRB; NIHS0319 - Discontinued
Cell line databases/resources CLO; CLO_0001777
CLO; CLO_0001928
MCCL; MCC:0000496
CLDB; cl5049
CCRID; 3101MOUTCM40
Anatomy/cell type resources BTO; BTO_0003353
Encyclopedic resources Wikidata; Q52183429
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number21