Cellosaurus cell line bEnd.3 (CVCL_0170)

Cellosaurus bEnd.3 (CVCL_0170)

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Cell line name

bEnd.3

Synonyms

bEND.3; b.End3; Bend.3; bEnd3; BEND-3; BEND3; brain-derived Endothelial cells.3

Accession

CVCL_0170

Resource Identification Initiative

To cite this cell line use: bEnd.3 (RRID:CVCL_0170)

Comments

Characteristics: Widely used for blood vascular research.
Doubling time: 26-30 hours (PubMed=14568334).
Genetic integration: Method=Transduction; Gene=UniProtKB; P03077; Murine polyomavirus (strain A2) (MPyV) middle T antigen (PyMT).
Transformant: NCBI_TaxID; 10636; Murine polyomavirus (strain A2) (MPyV).
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Cell type: Endothelial cell; CL=CL_0000115.

Species of origin

Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.

Hierarchy

Children:

CVCL_E1JC (HyCyte bEnd.3 KO-mCdh5)

CVCL_F1N1 (HyCyte bEnd.3 KO-mSpp1)

Sex of cell

Sex unspecified

Age at sampling

6W

Category

Transformed cell line

Web pages

Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-crl-2299
Info; Wikipedia; -; https://en.wikipedia.org/wiki/BEnd.3

Publications

PubMed=2379237; DOI=10.1016/0092-8674(90)90009-4
Roberto Montesano, Michael Sean Pepper, Uta Mohle-Steinlein, Werner Risau, Erwin Friedrich Wagner, Lelio Orci;
Increased proteolytic activity is responsible for the aberrant morphogenetic behavior of endothelial cells expressing the middle T oncogene.
Cell 62:435-445(1990)

PubMed=14568334; DOI=10.1016/S0006-8993(03)03443-7
Yadollah Omidi, Lee Campbell, Jaleh Barar, David Connell, Saeed Akhtar, Mark Gumbleton;
Evaluation of the immortalised mouse brain capillary endothelial cell line, b.End3, as an in vitro blood-brain barrier model for drug uptake and transport studies.
Brain Res. 990:95-112(2003)

PubMed=22224974; DOI=10.1089/scd.2011.0501
Katherine Joubin, Amelia Richardson, Natalia Novoa, Edmund Tu, Mark J. Tomishima;
The endothelial cell line bEnd.3 maintains human pluripotent stem cells.
Stem Cells Dev. 21:2312-2321(2012)

PubMed=25282873; PMCID=PMC4636729
David Yuen, Rose Kristine Leu, Julie Tse, S. Wang, L.L. Chen, Lu Chen;
Novel characterization of bEnd.3 cells that express lymphatic vessel endothelial hyaluronan receptor-1.
Lymphology 47:73-81(2014)

Cross-references

Cell line collections (Providers)

AddexBio; C0005030/5019
ATCC; CRL-2299
BCRC; 60515
ECACC; 96091929
JCRB; NIHS0319 - Discontinued

Cell line databases/resources

CLO; CLO_0001777
CLO; CLO_0001928
MCCL; MCC:0000496
CLDB; cl5049
CCRID; 3101MOUSCSP5267
CCRID; 3101MOUTCM40
CCRID; 6101MOU-CERC000126

Anatomy/cell type resources

BTO; BTO_0003353

Encyclopedic resources

Wikidata; Q52183429

Entry history

Entry creation

04-Apr-2012

Last entry update

27-Nov-2025

Version number

22