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Cellosaurus SK-MEL-30 (CVCL_0039)

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Cell line name SK-MEL-30
Synonyms SK-Mel-30; SK Mel 30; SKMEL-30; Sk Mel30; SKMEL30; AW-Mel
Accession CVCL_0039
Resource Identification Initiative To cite this cell line use: SK-MEL-30 (RRID:CVCL_0039)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: Human Protein Atlas, Cell Atlas panel.
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK1980-526.
Population: Caucasian.
Characteristics: Pigmented.
Doubling time: ~30 hours (DSMZ=ACC-151).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Expression; Large antibody panel staining analysis.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Sequence variations
  • Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Heterozygous (PubMed=9598804).
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Gly1339Arg (c.4015G>C); ClinVar=VCV000233611; Zygosity=Heterozygous (Cosmic-CLP=909726; DepMap=ACH-000810).
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Gln1406Ter (c.4216C>T); ClinVar=VCV000142519; Zygosity=Heterozygous (Cosmic-CLP=909726; DepMap=ACH-000810).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Pro114Leu (c.341C>T) (p.Ala128Ala, c.384C>T); ClinVar=VCV000077637; Zygosity=Homozygous (PubMed=9598804; Cosmic-CLP=909726; DepMap=ACH-000810).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (PubMed=10766161; PubMed=15009714; PubMed=21725359; PubMed=24576830; Cosmic-CLP=909726; DepMap=ACH-000810).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (PubMed=31068700).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Thr284Argfs*21 (c.851_852delCA) (p.R283fs, c.849_850del2); Zygosity=Heterozygous (Cosmic-CLP=909726; DepMap=ACH-000810).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,02:01
HLA-BB*08:01,44:02
HLA-CC*05:01,07:06
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North1.6
East Asian, South0
South Asian0
European, North62.41
European, South35.99
Disease Cutaneous melanoma (NCIt: C3510)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 67Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=909726; DSMZ=ACC-151; PubMed=25877200

Markers:
AmelogeninX,Y
CSF1PO10
D2S133819,21
D3S135815,16
D5S8189,12
D7S8208,9
D8S11798,14
D13S3178,11
D16S5398,11
D18S5112,14 (DSMZ=ACC-151)
13,15 (PubMed=25877200)
D19S43314,15
D21S1129,31.2
FGA20,21
Penta D12
Penta E5,14
TH016
TPOX8,11
vWA15,17

Run an STR similarity search on this cell line
Web pages Info; HPA; -; https://www.proteinatlas.org/learn/cellines
Provider; MSKCC; -; https://www.mskcc.org/research-advantage/support/technology/tangible-material/sk-mel-30-human-melanoma-cell-line
Publications

PubMed=978138; DOI=10.1084/jem.144.4.873; PMCID=PMC2190439
Hiroshi Shiku, Toshitada Takahashi, Herbert Friedrich Oettgen, Lloyd John Old;
Cell surface antigens of human malignant melanoma. II. Serological typing with immune adherence assays and definition of two new surface antigens.
J. Exp. Med. 144:873-881(1976)

PubMed=1067619; DOI=10.1073/pnas.73.9.3278; PMCID=PMC431008
Thomas E. Carey, Toshitada Takahashi, Lois A. Resnick-Silverman, Herbert Friedrich Oettgen, Lloyd John Old;
Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells.
Proc. Natl. Acad. Sci. U.S.A. 73:3278-3282(1976)

PubMed=6933476; DOI=10.1073/pnas.77.7.4260; PMCID=PMC349812
Alan N. Houghton, Michael C. Taormina, Hisami Ikeda, Tadashi Watanabe, Herbert Friedrich Oettgen, Lloyd John Old;
Serological survey of normal humans for natural antibody to cell surface antigens of melanoma.
Proc. Natl. Acad. Sci. U.S.A. 77:4260-4264(1980)

DOI=10.1007/978-1-4615-7228-2_39
Alan N. Houghton, Herbert Friedrich Oettgen, Lloyd John Old;
Malignant melanoma. Current status of the search for melanoma-specific antigens.
(In book chapter) Immunodermatology. Comprehensive Immunology, Vol 7; Safai, Bijan & Good, Robert Alan (eds.); pp.557-576; Springer; Boston; USA (1981)

PubMed=7175440; DOI=10.1084/jem.156.6.1755; PMCID=PMC2186870
Alan N. Houghton, Magdalena Eisinger, Anthony P. Albino, J. Gregory Cairncross, Lloyd John Old;
Surface antigens of melanocytes and melanomas. Markers of melanocyte differentiation and melanoma subsets.
J. Exp. Med. 156:1755-1766(1982)

PubMed=6864164; DOI=10.1084/jem.158.1.53; PMCID=PMC2187084
Alan N. Houghton, Hannah Brooks, Richard J. Cote, Michael C. Taormina, Herbert Friedrich Oettgen, Lloyd John Old;
Detection of cell surface and intracellular antigens by human monoclonal antibodies. Hybrid cell lines derived from lymphocytes of patients with malignant melanoma.
J. Exp. Med. 158:53-65(1983)

PubMed=3081645; DOI=10.4049/jimmunol.136.7.2534
Mounanandham Panneerselvam, Sydney Welt, Lloyd John Old, Carl-Wilhelm Vogel;
A molecular mechanism of complement resistance of human melanoma cells.
J. Immunol. 136:2534-2541(1986)

PubMed=3518877; DOI=10.3109/07357908609038260
Jorgen Fogh;
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3473501; DOI=10.1073/pnas.84.12.4200; PMCID=PMC305052
Sydney Welt, Michael Jules Mattes, Roberto Grando, Timothy M. Thomson, R.W. Leonard, Pat B. Zanzonico, Rodney Errol Bigler, Samuel D.J. Yeh, Herbert Friedrich Oettgen, Lloyd John Old;
Monoclonal antibody to an intracellular antigen images human melanoma transplants in nu/nu mice.
Proc. Natl. Acad. Sci. U.S.A. 84:4200-4204(1987)

PubMed=1716514
Anthony P. Albino, Brigid M. Davis, David Michael Nanus;
Induction of growth factor RNA expression in human malignant melanoma: markers of transformation.
Cancer Res. 51:4815-4820(1991)

PubMed=7509835; DOI=10.1111/1523-1747.ep12371784
Brigitte Bouchard, Setaluri Vijayasaradhi, Alan N. Houghton;
Production and characterization of antibodies against human tyrosinase.
J. Invest. Dermatol. 102:291-295(1994)

PubMed=9598804; DOI=10.1002/(SICI)1098-2264(199806)22:2<157::AID-GCC11>3.0.CO;2-N
Graeme J. Walker, Jose F. Flores, J. Michael Glendening, Amy Hsiu-Ti Lin, Isabel D.C. Markl, Jane W. Fountain;
Virtually 100% of melanoma cell lines harbor alterations at the DNA level within CDKN2A, CDKN2B, or one of their downstream targets.
Genes Chromosomes Cancer 22:157-163(1998)

PubMed=10766161
Hensin Tsao, Xue Zhang, Kianna Fowlkes, Frank G. Haluska;
Relative reciprocity of NRAS and PTEN/MMAC1 alterations in cutaneous melanoma cell lines.
Cancer Res. 60:1800-1804(2000)

PubMed=11668190; DOI=10.1177/002215540104901105
Hilmar Quentmeier, Mary Osborn, Julia Reinhardt, Margarete Zaborski, Hans Gunther Drexler;
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=15009714; DOI=10.1046/j.0022-202X.2004.22243.x; PMCID=PMC2586668
Hensin Tsao, Vikas Goel, Heng Wu, Guang Yang, Frank G. Haluska;
Genetic interaction between NRAS and BRAF mutations and PTEN/MMAC1 inactivation in melanoma.
J. Invest. Dermatol. 122:337-341(2004)

PubMed=16957166; DOI=10.1369/jhc.6A7001.2006; PMCID=PMC3958123
Ann-Catrin Andersson, Sara Stromberg, Helena Backvall, Caroline Kampf, Mathias Uhlen, Kenneth Wester, Fredrik Ponten;
Analysis of protein expression in cell microarrays: a tool for antibody-based proteomics.
J. Histochem. Cytochem. 54:1413-1423(2006)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=21725359; DOI=10.1038/onc.2011.250; PMCID=PMC3267014
Feng Xing, Yogindra Persaud, Christine A. Pratilas, Barry Stephen Taylor, Manickam Janakiraman, Qing-Bai She, Humilidad F. Gallardo, C. Liu, Taha Merghoub, Blake E. Hefter, Igor Dolgalev ...Show all 19 authors... , Agnes J. Viale, Adriana Heguy, Elisa de Stanchina, David Cobrinik, Gideon Bollag, Jedd D. Wolchok, Alan N. Houghton, David B. Solit; Show fewer authors
Concurrent loss of the PTEN and RB1 tumor suppressors attenuates RAF dependence in melanomas harboring (V600E)BRAF.
Oncogene 31:446-457(2012)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24576830; DOI=10.1158/0008-5472.CAN-13-2625; PMCID=PMC4005042
Moriah Heller Nissan, Christine A. Pratilas, Alexis M. Jones, Ricardo Ramirez, Helen Won, Cai-Lian Liu, Shakuntala Tiwari, Li Kong, Aphrothiti J. Hanrahan, Zhan Yao, Taha Merghoub ...Show all 19 authors... , Antoni Ribas, Paul B. Chapman, Rona D. Yaeger, Barry Stephen Taylor, Nikolaus Schultz, Michael F. Berger, Neal Rosen, David B. Solit; Show fewer authors
Loss of NF1 in cutaneous melanoma is associated with RAS activation and MEK dependence.
Cancer Res. 74:2340-2350(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30629668; DOI=10.1371/journal.pone.0210404; PMCID=PMC6328144
Cord C. Uphoff, Claudia Pommerenke, Sabine A. Denkmann, Hans Gunther Drexler;
Screening human cell lines for viral infections applying RNA-Seq data analysis.
PLoS ONE 14:e0210404.1-e0210404.22(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
David Paul Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald 3rd, Marian Kalocsay, Judit Jane-Valbuena, Ellen T. Gelfand, Devin K. Schweppe, Mark P. Jedrychowski, Javad Golji ...Show all 20 authors... , Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi Alexander Garraway, William Raj Sellers, Steven P. Gygi; Show fewer authors
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
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Cross-references
Cell line collections (Providers) DSMZ; ACC-151
Cell line databases/resources CLO; CLO_0009045
CLDB; cl4323
cancercelllines; CVCL_0039
Cell_Model_Passport; SIDM00392
Cosmic-CLP; 909726
DepMap; ACH-000810
DSMZCellDive; ACC-151
LINCS_LDP; LCL-1257
Anatomy/cell type resources BTO; BTO_0002133
Biological sample resources BioSample; SAMN03473251
BioSample; SAMN10988313
CRISP screens repositories BioGRID_ORCS_Cell_line; 465
Chemistry resources ChEMBL-Cells; CHEMBL3308128
ChEMBL-Targets; CHEMBL1075577
GDSC; 909726
PharmacoDB; SKMEL30_1398_2019
PubChem_Cell_line; CVCL_0039
Encyclopedic resources Wikidata; Q54954059
Experimental variables resources EFO; EFO_0006488
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM784510
GEO; GSM887586
GEO; GSM888669
GEO; GSM1670441
GEO; GSM7701406
GEO; GSM7701407
Polymorphism and mutation databases Cosmic; 706090
Cosmic; 721825
Cosmic; 851035
Cosmic; 888061
Cosmic; 888834
Cosmic; 909726
Cosmic; 933128
Cosmic; 1022283
Cosmic; 1060425
Cosmic; 1669146
IARC_TP53; 27239
LiGeA; CCLE_671
Progenetix; CVCL_0039
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
EGA; EGAS00001002554
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number47