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Cellosaurus AN3-CA (CVCL_0028)

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Cell line name AN3-CA
Synonyms AN3_CA; AN3 CA; AN3 Ca; AN3CA; AN-3; AN3; Acanthosis Nigricans 3rd attempt-CArcinoma
Accession CVCL_0028
Resource Identification Initiative To cite this cell line use: AN3-CA (RRID:CVCL_0028)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: ERK genetic alteration cell panel (ATCC TCP-1033).
Part of: Human Protein Atlas, Cell Atlas panel.
Part of: MD Anderson Cell Lines Project.
Part of: PTEN genetic alteration cell panel (ATCC TCP-1030).
Population: Caucasian.
Doubling time: ~45-50 hours (CLS=300119); ~55 hours (DSMZ=ACC-267).
Microsatellite instability: Instable (MSI-high) (PubMed=31068700; Sanger).
Omics: Genomics; Chromatin accessibility; ATAC-seq.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Expression; Large antibody panel staining analysis.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Lymph node; UBERON=UBERON_0000029.
Sequence variations
  • Mutation; HGNC; HGNC:6877; MAPK3; Simple; p.Pro373Ser (c.1117C>T); Zygosity=Heterozygous (ATCC=HTB-111).
  • Mutation; HGNC; HGNC:8979; PIK3R1; Simple; p.Arg557_Lys561delArgGluIleAspLysinsGln (c.1670_1681delGAGAAATTGACA); Zygosity=Heterozygous (ATCC=HTB-111).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Arg130Glnfs*4 (c.389delG); ClinVar=VCV000192224; Zygosity=Homozygous (PubMed=20944090; ATCC=HTB-111).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gly389Trp (c.1165G>T); ClinVar=VCV000156517; Zygosity=Heterozygous (ATCC=HTB-111).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*11:01,11:01
HLA-BB*15:11,58:01
HLA-CC*05:01,06:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.36
Native American0
East Asian, North0.85
East Asian, South1.08
South Asian3.21
European, North62.96
European, South31.54
Disease Acanthosis nigricans (NCIt: C26687)
Type I endometrial adenocarcinoma (NCIt: C40145)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 55Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-111; CLS=300119; Cosmic-CLP=910781; DSMZ=ACC-267; IZSLER=BS TCL 200; PubMed=25877200

Markers:
AmelogeninX
CSF1PO12,14 (DSMZ=ACC-267)
12,14,15 (ATCC=HTB-111; CLS=300119; Cosmic-CLP=910781; IZSLER=BS TCL 200; PubMed=25877200)
D2S133820,23
D3S135817
D5S81811,14
D7S8207,7.1,10 (ATCC=HTB-111; PubMed=25877200)
7,10 (CLS=300119; Cosmic-CLP=910781; IZSLER=BS TCL 200)
7.1,10 (DSMZ=ACC-267)
D8S117912,14
D13S31712,14
D16S53910,14 (ATCC=HTB-111; CLS=300119; DSMZ=ACC-267; PubMed=25877200)
10,14,15 (Cosmic-CLP=910781; IZSLER=BS TCL 200)
D18S5115,18
D19S43314
D21S1129,30
FGA23
Penta D9,11
Penta E9,16
TH019.3,10
TPOX8,10
vWA14,20

Run an STR similarity search on this cell line
Web pages Info; HPA; -; https://www.proteinatlas.org/learn/cellines
Info; MCLP; -; https://tcpaportal.org/mclp/
Publications

PubMed=14207855; DOI=10.1093/jnci/33.3.441
Clyde J. Dawe, William G. Banfield 2nd, Willie DeWard Morgan, Marilyn Shaw Slatick, Helen Ollendorf-Curth;
Growth in continuous culture, and in hamsters, of cells from a neoplasm associated with acanthosis nigricans.
J. Natl. Cancer Inst. 33:441-456(1964)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Jorgen Fogh, Jens M. Fogh, Thomas Orfeo;
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=6220172
Nicholas C. Dracopoli, Jorgen Fogh;
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=3518877; DOI=10.3109/07357908609038260
Jorgen Fogh;
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=11668190; DOI=10.1177/002215540104901105
Hilmar Quentmeier, Mary Osborn, Julia Reinhardt, Margarete Zaborski, Hans Gunther Drexler;
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=16957166; DOI=10.1369/jhc.6A7001.2006; PMCID=PMC3958123
Ann-Catrin Andersson, Sara Stromberg, Helena Backvall, Caroline Kampf, Mathias Uhlen, Kenneth Wester, Fredrik Ponten;
Analysis of protein expression in cell microarrays: a tool for antibody-based proteomics.
J. Histochem. Cytochem. 54:1413-1423(2006)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20218740; DOI=10.1177/153303461000900207; PMCID=PMC5120669
Ying-Mei Wang, Da Yang, David Cogdell, Li-Mei Hu, Feng-Xia Xue, Russell Broaddus, Wei Zhang;
Genomic characterization of gene copy-number aberrations in endometrial carcinoma cell lines derived from endometrioid-type endometrial adenocarcinoma.
Technol. Cancer Res. Treat. 9:179-189(2010)

PubMed=20944090; DOI=10.1126/scitranslmed.3001538
Konstantin J. Dedes, Daniel Wetterskog, Ana M. Mendes-Pereira, Rachael Natrajan, Maryou B. Lambros, Felipe Correa Geyer, Radost Vatcheva, Kay Savage, Alan Mackay, Christopher J. Lord, Alan Ashworth, Jorge Sergio Reis-Filho;
PTEN deficiency in endometrioid endometrial adenocarcinomas predicts sensitivity to PARP inhibitors.
Sci. Transl. Med. 2:53ra75.1-53ra75.8(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017; PMCID=PMC3432677
Christopher T. Korch, Monique A. Spillman, Twila A. Jackson, Britta M. Jacobsen, Susan K. Murphy, Bruce Arthur Lessey, Virgil Craig Jordan, Andrew Peter Bradford;
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Gynecol. Oncol. 127:241-248(2012)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; HTB-111
BCRJ; 0363
CCTCC; GDC0127
CLS; 300119
DSMZ; ACC-267
IZSLER; BS TCL 200
Cell line databases/resources CLO; CLO_0001722
CLO; CLO_0037160
cancercelllines; CVCL_0028
CCRID; 4201HUM-CCTCC00127
Cell_Model_Passport; SIDM00901
Cosmic-CLP; 910781
DepMap; ACH-000940
DSMZCellDive; ACC-267
IGRhCellID; AN3CA
LINCS_LDP; LCL-1499
TOKU-E; 543
Anatomy/cell type resources BTO; BTO_0001771
Biological sample resources BioSample; SAMN03471092
BioSample; SAMN03473131
BioSample; SAMN03473336
BioSample; SAMN10988375
Chemistry resources ChEMBL-Cells; CHEMBL3308708
ChEMBL-Targets; CHEMBL1075392
GDSC; 910781
PharmacoDB; AN3CA_63_2019
PubChem_Cell_line; CVCL_0028
Encyclopedic resources Wikidata; Q54749896
Experimental variables resources EFO; EFO_0002110
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM374970
GEO; GSM375429
GEO; GSM827494
GEO; GSM886869
GEO; GSM887934
GEO; GSM1669600
GEO; GSM3161710
GEO; GSM3161711
GEO; GSM7701431
GEO; GSM7701432
Polymorphism and mutation databases Cosmic; 687550
Cosmic; 699283
Cosmic; 713473
Cosmic; 713495
Cosmic; 809134
Cosmic; 846176
Cosmic; 871551
Cosmic; 889116
Cosmic; 910781
Cosmic; 980634
Cosmic; 1007162
Cosmic; 1057760
Cosmic; 1066221
Cosmic; 1070810
Cosmic; 1102376
Cosmic; 1105620
Cosmic; 1177683
Cosmic; 1223484
Cosmic; 1434192
Cosmic; 1576450
Cosmic; 1622894
Cosmic; 1696752
Cosmic; 2030460
Cosmic; 2646618
IARC_TP53; 21175
LiGeA; CCLE_574
Progenetix; CVCL_0028
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number46