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Cellosaurus SEM (CVCL_0095)

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Cell line name SEM
Accession CVCL_0095
Resource Identification Initiative To cite this cell line use: SEM (RRID:CVCL_0095)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: LL-100 blood cancer cell line panel.
Population: Caucasian.
Doubling time: ~48 hours (PubMed=8199015); 24 hours (PubMed=25984343); ~30 hours (DSMZ=ACC-546).
Microsatellite instability: Instable (MSI) (PubMed=31068700).
Omics: Deep exome analysis.
Omics: Deep quantitative phosphoproteome analysis.
Omics: Deep quantitative proteome analysis.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene fusion; HGNC; 7135; AFF1 + HGNC; 7132; KMT2A; Name(s)=KMT2A-AFF1, MLL-AFF1, ALL-1/AF4 (PubMed=14671638; PubMed=35354797).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.His83Tyr (c.247C>T) (p.Ala97Val, c.290C>T); ClinVar=VCV000376307; Zygosity=Heterozygous (DepMap=ACH-000782).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Heterozygous (DepMap=ACH-000782).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,32:01
HLA-BB*08:01,15:01
HLA-CC*03:03,07:01

Source: DSMZCellDive=ACC-546
Class I
HLA-AA*01:01:01,32:01:01
HLA-BB*08:01:01,15:01:01
HLA-CC*03:03:01,07:01:01
Class II
HLA-DPDPA1*01:03:01,01:03:01
DPB1*04:02:01,02:01:02
HLA-DQDQA1*01:03:01,01:03:01
DQB1*03:01:01,06:03:01
HLA-DRDRA*01:01:01,01:02:02
DRB1*11:01:01,13:01:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.88
Native American0.05
East Asian, North2.53
East Asian, South0
South Asian0.07
European, North64.12
European, South32.35
Disease Childhood B acute lymphoblastic leukemia (NCIt: C9140)
B acute lymphoblastic leukemia with t(v;11q23.3) MLL rearranged (NCIt: C80342)
B-lymphoblastic leukemia/lymphoma with t(v;11q23.3) (ORDO: Orphanet_585918)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_S906 (SEMK2)
Sex of cell Female
Age at sampling 5Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-546

Markers:
AmelogeninX
CSF1PO11,12
D2S133817,24
D3S135814,17
D5S81812
D7S82010,12
D8S117911,14
D13S31714
D16S5398,10
D18S5116,17
D19S43313,14
D21S1129,30,31
FGA21,25
Penta D11,14
Penta E10,11
TH016,10
TPOX8
vWA17

Run an STR similarity search on this cell line
Publications

PubMed=8199015; DOI=10.1111/j.1365-2141.1994.tb04726.x
Greil J., Gramatzki M., Burger R., Marschalek R., Peltner M., Trautmann U., Hansen-Hagge T.E., Bartram C.R., Fey G.H., Stehr K., Beck J.-D.
The acute lymphoblastic leukaemia cell line SEM with t(4;11) chromosomal rearrangement is biphenotypic and responsive to interleukin-7.
Br. J. Haematol. 86:275-283(1994)

PubMed=7794749; DOI=10.1111/j.1365-2141.1995.tb05151.x
Marschalek R., Greil J., Lochner K., Nilson I., Siegler G., Zweckbronner I., Beck J.-D., Fey G.H.
Molecular analysis of the chromosomal breakpoint and fusion transcripts in the acute lymphoblastic SEM cell line with chromosomal translocation t(4;11).
Br. J. Haematol. 90:308-320(1995)

PubMed=9067587; DOI=10.1038/sj.leu.2400571
Uphoff C.C., MacLeod R.A.F., Denkmann S.A., Golub T.R., Borkhardt A., Janssen J.W.G., Drexler H.G.
Occurrence of TEL-AML1 fusion resulting from (12;21) translocation in human early B-lineage leukemia cell lines.
Leukemia 11:441-447(1997)

PubMed=9881706; DOI=10.1038/sj.onc.1202229
Reichel M., Gillert E., Nilson I., Siegler G., Greil J., Fey G.H., Marschalek R.
Fine structure of translocation breakpoints in leukemic blasts with chromosomal translocation t(4;11): the DNA damage-repair model of translocation.
Oncogene 17:3035-3044(1998)

DOI=10.1016/B978-0-12-221970-2.50457-5
Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=14671638; DOI=10.1038/sj.leu.2403236
Drexler H.G., Quentmeier H., MacLeod R.A.F.
Malignant hematopoietic cell lines: in vitro models for the study of MLL gene alterations.
Leukemia 18:227-232(2004)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=21552520; DOI=10.1371/journal.pone.0019169; PMCID=PMC3084268
Gu T.-L., Nardone J., Wang Y., Loriaux M., Villen J., Beausoleil S.A., Tucker M., Kornhauser J.M., Ren J.-M., MacNeill J., Gygi S.P., Druker B.J., Heinrich M.C., Rush J., Polakiewicz R.D.
Survey of activated FLT3 signaling in leukemia.
PLoS ONE 6:E19169-E19169(2011)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5; PMCID=PMC6167786
Tan K.-T., Ding L.-W., Sun Q.-Y., Lao Z.-T., Chien W., Ren X., Xiao J.-F., Loh X.-Y., Xu L., Lill M., Mayakonda A., Lin D.-C., Yang H.H., Koeffler H.P.
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940.1-940.13(2018)

PubMed=30629668; DOI=10.1371/journal.pone.0210404; PMCID=PMC6328144
Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.
Screening human cell lines for viral infections applying RNA-Seq data analysis.
PLoS ONE 14:E0210404-E0210404(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31160637; DOI=10.1038/s41598-019-44491-x; PMCID=PMC6547646
Quentmeier H., Pommerenke C., Dirks W.G., Eberth S., Koeppel M., MacLeod R.A.F., Nagel S., Steube K.G., Uphoff C.C., Drexler H.G.
The LL-100 panel: 100 cell lines for blood cancer studies.
Sci. Rep. 9:8218-8218(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. 3rd, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=35354797; DOI=10.1038/s41467-022-29224-5; PMCID=PMC8967900
Leo I.R., Aswad L., Stahl M., Kunold E., Post F., Erkers T., Struyf N., Mermelekas G., Joshi R.N., Gracia-Villacampa E., Ostling P., Kallioniemi O.-P., Pokrovskaja Tamm K., Siavelis I., Lehtio J., Vesterlund M., Jafari R.
Integrative multi-omics and drug response profiling of childhood acute lymphoblastic leukemia cell lines.
Nat. Commun. 13:1691.1-1691.19(2022)

Cross-references
Cell line collections (Providers) DSMZ; ACC-546
Cell line databases/resources cancercelllines; CVCL_0095
Cell_Model_Passport; SIDM01424
DepMap; ACH-000782
DSMZCellDive; ACC-546
IGRhCellID; SEM
Biological sample resources BioSample; SAMN03473532
BioSample; SAMN10988273
CRISP screens repositories BioGRID_ORCS_Cell_line; 1548
Chemistry resources ChEMBL-Cells; CHEMBL4295399
ChEMBL-Targets; CHEMBL4296493
PharmacoDB; SEM_1363_2019
PubChem_Cell_line; CVCL_0095
Encyclopedic resources Wikidata; Q54952795
Experimental variables resources EFO; EFO_0002326
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-7721
ArrayExpress; E-MTAB-7722
GEO; GSM887563
GEO; GSM888646
GEO; GSM5137743
GEO; GSM5137744
Polymorphism and mutation databases Cosmic; 1130254
Cosmic; 2491076
Cosmic; 2542839
IARC_TP53; 28231
LiGeA; CCLE_736
Progenetix; CVCL_0095
Proteomic databases PRIDE; PXD023662
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number31