Molecular Cytogenetics of Common Epithelial Cancers > Lung Carcinoma cell lines > NCI-H2009

NCI-H2009   [Cellosaurus entry CVCL_1514]

Lung Cancer Cell Line

Description

A hyper-diploid cell line from lung, metastatic site, lymph node adenocarcinoma, held at ATCC. Karyotyped in collaboration with the Sanger Institute.

   
Notable Features

Extremely rearranged with 27 structural abnormalities. Translocations involve chromosomes 1, 2, 3, 4, 5, 6, 7, 11, 12, 13, 14, 15, 16, 17, 19, 20, 22. No normal chromosomes 1, 6, 13, 14, 18, 22 were found. Loss of chromosome 13 and del (13). Most rearrangements unbalanced, but two reciprocal translocations observed – t(3;6)(q24;q21) and t(12;17)(p10;p10). All cells contained double minutes (DM), ranging from 1-8 per cell. Consistent DM were found for chromosomes 13, 18, 5, 16.

   
Representative SKY Karyotype
   
   

48(47-49), X, -X, i (1p), der(1)t(13;1;X), der(1)t(1;21), der(1)del(1), 2x2, 3x1, der(3)t(3;6)*; 4x1, der(4;19)(p10;p10?), der(4)t(4;13), 5x1, i(5p)x2, der(6)t(3;6)*, 7x1, der(7)t(6;7)dup(7)(q21?-qter), 8x1, i(8q), 9x2, 10x2, 11x1, der(11)t(11;13), der(11)t(6;11), 12x1, der(12;17)(p10;p10)**, der(13)del(13), der(5;14)(q10-qter :: q?), der(14)t(X;14), 15x1, der(15)t(15;2;19), 16x1, der(16)t(2;16), der(16)del(16), 17x1, der(12;17)(q10;q10)**, i(18q), 19x1, der(19)t(17;19;1), 20x1, der(9;20)(q10;q10?), der(20)t(16;20), 21x1, der(22)t(5;22), der(22)t(13;22), dmin

* reciprocal translocation ** reciprocal translocation

   
 
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Lung Cancer Cell Lines