ID   HEK293T HA-PEROXO
AC   CVCL_ZM07
SY   HEK-293T HA-PEROXO
DR   PRIDE; PXD018880
DR   PRIDE; PXD018918
DR   Wikidata; Q98126676
RX   PubMed=32417403;
CC   Characteristics: Expresses PEROXO-Tag, a chimeric protein consisting of three HA epitopes attached to the N-terminus of mEGFP and a segment of PEX26 fused to the EGFP C-terminus. This tag allows for localization to and insertion into the peroxisomal membrane.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; QKFJN; mEGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:22965; PEX26 (Note=With p.Met1_Val236del).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Omics: Proteomics; Subcellular; Peroxisome; Quantitative.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0063 ! HEK293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 02-07-20; Last updated: 10-04-25; Version: 10
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RX   PubMed=32417403; DOI=10.1016/j.isci.2020.101109; PMCID=PMC7254474;
RA   Ray G.J., Boydston E.A., Shortt E., Wyant G.A., Lourido S., Chen W.W.,
RA   Sabatini D.M.;
RT   "A PEROXO-Tag enables rapid isolation of peroxisomes from human
RT   cells.";
RL   iScience 23:101109.1-101109.9(2020).
//