ID   Tango EDG8-bla U2OS
AC   CVCL_ZK33
DR   Wikidata; Q98133119
RX   PubMed=19726785;
WW   Provider; Thermo Fisher; K1518; https://www.thermofisher.com/order/catalog/product/K1518
CC   Population: Caucasian.
CC   Characteristics: The target GPCR is linked to a TEV protease cleavage site and a GAL4-VP16 transcription factor. When activated the GPCR recruits the beta-arrestin/TEV protease fusion protein, the TEV protease cleaves off the transcription factor that translocates to the nucleus to activate the expression of the bla gene.
CC   Characteristics: Stably expresses a beta-arrestin/TEV protease fusion protein and a beta-lactamase reporter gene under the control of an upstream activator sequence (UAS).
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:712; ARRB2.
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:14299; S1PR5.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P62593; Escherichia coli beta-lactamase bla (Note=With p.Met1_Phe22del, p.Ala23Met, p.Val82Ile and p.Met184Val).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04517; Tobacco etch virus (TEV) protease (Note=AA 2038-2279).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04386; Yeast GAL4 (Note=With p.Ile148_Glu881del = DNA-binding domain).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_ZJ93 ! Tango GPCR-bla U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 02-07-20; Last updated: 10-04-25; Version: 10
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RX   PubMed=19726785; DOI=10.1177/1087057109343809;
RA   Wetter J.A., Revankar C., Hanson B.J.;
RT   "Utilization of the Tango beta-arrestin recruitment technology for
RT   cell-based EDG receptor assay development and interrogation.";
RL   J. Biomol. Screen. 14:1134-1141(2009).
//