ID   CHO-K1 UA10
AC   CVCL_ZE99
SY   UA10
DR   Wikidata; Q98125620
RX   PubMed=32346859;
CC   Characteristics: Doubly auxotrophic; deficient in 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) and uridine monophosphate synthetase (UMPS), respectively disrupting the purine and pyrimidine de novo synthesis pathways.
CC   Characteristics: Useful for the production of recombinant monoclonal antibodies as it rovides a rapid and efficient selection method for the serial or simultaneous transfer of genes for multiple polypeptides of choice into the cells using readily available purine- and pyrimidine-free commercial media.
CC   Knockout cell: Method=CRISPR/Cas9; UniProtKB; G3I1V4; Atic.
CC   Knockout cell: Method=CRISPR/Cas9; UniProtKB; G3GSP0; Umps.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_0214 ! CHO-K1
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 02-07-20; Last updated: 30-01-24; Version: 5
//
RX   PubMed=32346859; DOI=10.1002/bit.27367;
RA   Zhang Q.-H., Jiang B., Du Z.-M., Chasin L.A.;
RT   "A doubly auxotrophic CHO-K1 cell line for the production of
RT   recombinant monoclonal antibodies.";
RL   Biotechnol. Bioeng. 117:2401-2409(2020).
//