ID   B2M-/Edimer(preCre)Elf1
AC   CVCL_YY08
DR   WiCell; b2m-edimer-pre-cre-elf1
DR   Wikidata; Q98125533
RX   PubMed=28504668;
CC   Characteristics: Using a rAAV, the second B2M allele has been edited to express B2M covalently linked at the C-terminus to a flexible (G4S)4 linker followed by HLA-E. A HyTK-Neo selection cassette has not been removed by Cre-recombinase.
CC   Knockout cell: Method=Recombinant Adeno-Associated Virus; HGNC; HGNC:914; B2M (Note=1 of 2 alleles).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C115 ! Elf1
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 02-07-20; Last updated: 14-08-25; Version: 8
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RX   PubMed=28504668; DOI=10.1038/nbt.3860; PMCID=PMC5548598;
RA   Gornalusse, German G.
RA   Hirata, Roli K.
RA   Funk, Sarah E.
RA   Riolobos, Laura
RA   Lopes, Vanda S.
RA   Manske, Gabriel
RA   Prunkard, Donna
RA   Colunga, Aric G.
RA   Hanafi, Laila-Aicha
RA   Clegg, Dennis O.
RA   Turtle, Cameron
RA   Russell, David W.
RT   "HLA-E-expressing pluripotent stem cells escape allogeneic responses
RT   and lysis by NK cells.";
RL   Nat. Biotechnol. 35:765-772(2017).
//