ID   U2OS MPX-Nomad ETBR
AC   CVCL_YK70
SY   MPXNomad ETBR; MultiPleXed-Nomad ETBR U2OS
DR   Wikidata; Q98133758
RX   PubMed=29412765;
CC   Population: Caucasian.
CC   Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal.
CC   Characteristics: The red cAMP-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboFP650 fluorescent protein, a Ht31 peptide fused to a KDEL signal.
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; JS838; TurboFP650 (Note=Far-red fluorescent protein derived from eqFP578).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; A315M; TurboGFP (Note=Green fluorescent protein from P.plumata).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:3180; EDNRB.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_YK69 ! U2OS MPX-Nomad
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 19-12-19; Last updated: 10-04-25; Version: 12
//
RX   PubMed=29412765; DOI=10.1177/2472630318754828;
RA   Mella R.M., Kortazar D., Roura-Ferrer M., Salado C., Valcarcel M.,
RA   Castilla A., Villace P.;
RT   "Nomad biosensors: a new multiplexed technology for the screening of
RT   GPCR ligands.";
RL   SLAS Technol. 23:207-216(2018).
//