ID   U2OS MPX-Nomad
AC   CVCL_YK69
SY   MPXNomad; MultiPleXed-Nomad U2OS
DR   Wikidata; Q98133757
RX   PubMed=29412765;
CC   Population: Caucasian.
CC   Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal.
CC   Characteristics: The red cAMP-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboFP650 fluorescent protein, a Ht31 peptide fused to a KDEL signal.
CC   Sequence variation: Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: UniProtKB; J9PBR7; Entacmaea quadricolor far-red fluorescent protein eqFP650.
CC   Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 19-12-19; Last updated: 05-10-23; Version: 8
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RX   PubMed=29412765; DOI=10.1177/2472630318754828;
RA   Mella R.M., Kortazar D., Roura-Ferrer M., Salado C., Valcarcel M.,
RA   Castilla A., Villace P.;
RT   "Nomad biosensors: a new multiplexed technology for the screening of
RT   GPCR ligands.";
RL   SLAS Technol. 23:207-216(2018).
//