ID   U2OS TACR3 calcium-Nomad
AC   CVCL_YK60
SY   Ca++ Nomad TACR3 (NK3)/U2OS; NK3 (TACR3) Nomad; NK3 Nomad
DR   Innoprot; P70104
DR   Wikidata; Q98133766
CC   Population: Caucasian.
CC   Characteristics: In absence of calcium, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells.
CC   Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal.
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:11528; TACR3.
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; A315M; TurboGFP (Note=Green fluorescent protein from P.plumata).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 19-12-19; Last updated: 19-12-24; Version: 11
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