ID   U2OS CHRM5 DAG-Nomad
AC   CVCL_YK40
SY   DAG-NOMAD Muscarinic 5; DAGNomad M5; DAGNOMAD M5
DR   Innoprot; P70603
DR   Wikidata; Q98133740
CC   Population: Caucasian.
CC   Characteristics: In absence of DAG, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells.
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; JS838; TurboFP650 (Note=Far-red fluorescent protein derived from eqFP578).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:1954; CHRM5.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 19-12-19; Last updated: 10-04-25; Version: 12
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