ID   HEK293 CCKBR calcium-Nomad
AC   CVCL_YK06
SY   CCKBR Ca2+Nomad; CCK2 Nomad
DR   Innoprot; P70108
DR   Wikidata; Q94092849
CC   Characteristics: In absence of calcium, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells.
CC   Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal.
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; A315M; TurboGFP (Note=Green fluorescent protein from P.plumata).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:1571; CCKBR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0045 ! HEK293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 19-12-19; Last updated: 14-08-25; Version: 13
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