ID   CHSE-GS2
AC   CVCL_YI85
SY   GS2
DR   Wikidata; Q93453361
RX   PubMed=31142600;
CC   Group: Fish cell line.
CC   Characteristics: The parent cell line was transiently transfected with a mix of sgRNAs designed against stat2 and against mEGFP. mEGFP-deficient cells were isolated (PubMed=31142600).
CC   Knockout cell: Method=CRISPR/Cas9; UniProtKB; A0A8C8HVT5; stat2.
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=Nuclear version; nCas9n).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Epithelial cell; CL=CL_0000066.
OX   NCBI_TaxID=74940; ! Oncorhynchus tshawytscha (Chinook salmon)
HI   CVCL_DG46 ! CHSE-EC
SX   Sex unspecified
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 19-12-19; Last updated: 10-04-25; Version: 8
//
RX   PubMed=31142600; DOI=10.4049/jimmunol.1801376; PMCID=PMC6612602;
RA   Dehler C.E., Lester K., Della Pelle G., Jouneau L., Houel A.,
RA   Collins C., Dovgan T., Machat R., Zou J., Boudinot P., Martin S.A.M.,
RA   Collet B.;
RT   "Viral resistance and IFN signaling in STAT2 knockout fish cells.";
RL   J. Immunol. 203:465-475(2019).
//