ID   MEF (DR4) MITC
AC   CVCL_Y468
SY   DR4 MEF MITC
DR   ATCC; SCRC-1045.2
DR   Wikidata; Q54905025
CC   Characteristics: Used as feeder cells to support the growth of stem cells in the undifferentiated state.
CC   Characteristics: Growth-arrested by mitomycin C.
CC   Characteristics: Expresses transgenes that make this cell line resistant to three different drugs: geneticin (G418), puromycin and hygromycin. KO of Hprt1 makes the cell line resistant to 6-tioguanine (from parent cell line).
CC   Knockout cell: Method=KO mouse; MGI; MGI:96217; Hprt1.
CC   Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: ChEBI; CHEBI_27504; Mitomycin C (MMC; MitC).
CC   Discontinued: ATCC; SCRC-1045.2; true.
CC   Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: DR4.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_5277 ! MEF (DR4)
SX   Mixed sex
AG   14FD
CA   Finite cell line
DT   Created: 07-11-14; Last updated: 10-04-25; Version: 13
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