ID   HEK293-DR-GFP-RAD51B-9/B+
AC   CVCL_XX06
DR   DSMZ; ACC-850
DR   DSMZCellDive; ACC-850
DR   Wikidata; Q94095046
CC   Characteristics: The parental RAD51B KO cell line is functionally reconstituted by retroviral transfection of RAD51B.
CC   Characteristics: Transfected with the hprtDR-GFP reporter construct that targets the DR-GFP into the hprt locus and includes a dominant selectable puroR marker. The DR-GFP construct which contains a tandem repeat of two inactive genes coding for EGFP. After transfection with an expression vector coding for the I-SceI enzyme, recombination induced by the cleavage of the I-SceI site recreates a functional EGFP.
CC   Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
CC   Doubling time: ~24-36 hours (DSMZ=ACC-850).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:9822; RAD51B.
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:9822; RAD51B.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
ST   Source(s): DSMZ=ACC-850
ST   Amelogenin: X
ST   CSF1PO: 12
ST   D13S317: 12,14
ST   D16S539: 9,13
ST   D18S51: 17,18
ST   D19S433: 18
ST   D21S11: 28,30.2
ST   D2S1338: 19
ST   D3S1358: 15,17
ST   D5S818: 8
ST   D7S820: 11,12
ST   D8S1179: 12,14
ST   FGA: 23
ST   Penta D: 9,10
ST   Penta E: 7,15
ST   TH01: 7,9.3
ST   TPOX: 11
ST   vWA: 16,19
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_XX01 ! HEK293-DR-GFP-RAD51B-9
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 19-12-19; Last updated: 10-04-25; Version: 13
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