ID   MHHi006-A-3
AC   CVCL_XJ45
SY   CFTRKO/CFTRdTomato89; HSC1285TiPS2_CFTRtomatoC89 (KO)
DR   hPSCreg; MHHi006-A-3
DR   Wikidata; Q95989465
RX   PubMed=31080112;
CC   From: Hannover Medical School, LEBAO (MHH); Hannover; Germany.
CC   Characteristics: Using TALEN a ZeoR-T2A-dTomato(nuc) construct was integrated in one of the alleles of CFTR which has a 4 bp deletion in its Kozak sequence (PubMed=31080112).
CC   Genetic integration: Method=TALEN; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_VN33 ! MHHi006-A
SX   Male
AG   <1D
CA   Induced pluripotent stem cell
DT   Created: 19-12-19; Last updated: 10-09-24; Version: 6
//
RX   PubMed=31080112; DOI=10.1016/j.stemcr.2019.04.014; PMCID=PMC6565754;
RA   Merkert S., Schubert M., Olmer R., Engels L., Radetzki S., Veltman M.,
RA   Scholte B.J., Zollner J., Pedemonte N., Galietta L.J.V.,
RA   von Kries J.P., Martin U.;
RT   "High-throughput screening for modulators of CFTR activity based on
RT   genetically engineered cystic fibrosis disease-specific iPSCs.";
RL   Stem Cell Reports 12:1389-1403(2019).
//