ID   S2R+-Gmap-GFP-7
AC   CVCL_XF67
DR   DGRC; 277
DR   FlyBase_Cell_line; FBtc0000277
DR   Wikidata; Q98129275
CC   Group: Insect cell line.
CC   From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA.
CC   Characteristics: The constitutively Cas9-expressed parent cell line was modified with a knock-in of eGFP into the Gmap locus (FlyBase_Gene=FBgn0027287).
CC   Characteristics: Transfected with a pMT-FLAG-Cas9 construct where the expression of N-terminally FLAG-tagged Cas9 is driven by a copper-inducible metallothionein promoter (from parent cell line).
CC   Genetic integration: Method=Protein trap; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With a N-termial FLAG tag).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_UD30 ! S2R+-MT::Cas9
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 06-09-19; Last updated: 14-08-25; Version: 8
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