ID   S2R+-Cnx99a-GFP-4
AC   CVCL_XF62
DR   DGRC; 273
DR   FlyBase_Cell_line; FBtc0000273
DR   Wikidata; Q98129270
CC   Group: Insect cell line.
CC   From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA.
CC   Characteristics: The constitutively Cas9-expressed parent cell line was modified with a knock-in of eGFP into the Cnx99a locus (FlyBase_Gene=FBgn0015622).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Breed/subspecies: Oregon-R.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_UD30 ! S2R+-MT::Cas9
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 06-09-19; Last updated: 30-01-24; Version: 6
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