ID   JE6-1REP-iNKT
AC   CVCL_WN97
DR   cancercelllines; CVCL_WN97
DR   Wikidata; Q94327309
RX   PubMed=31092850;
CC   Population: Caucasian.
CC   Characteristics: Fluorescence-based iNKT-TCR reporter system, applicable for screening potential new lipid antigens that infuence iNKT cell activation.
CC   Characteristics: The eGFP reporter gene is under control of a NF-kappaB promoter element.
CC   Sequence variation: Mutation; HGNC; HGNC:959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11491; SYK; Simple; p.Met34Hisfs*3 (c.98_99insG); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:12027; TRA (Note=iNKT-TCR V-alpha24).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:12155; TRB (Note=iNKT-TCR V-beta11).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
CC   Cell type: T-cell; CL=CL_0000084.
DI   NCIt; C7953; Childhood T acute lymphoblastic leukemia
DI   ORDO; Orphanet_99861; Precursor T-cell acute lymphoblastic leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_WN96 ! Jurkat E6.1 NF-kappaB::eGFP
SX   Male
AG   14Y
CA   Cancer cell line
DT   Created: 05-07-19; Last updated: 10-04-25; Version: 13
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RX   PubMed=31092850; DOI=10.1038/s41598-019-43529-4; PMCID=PMC6520406;
RA   Humeniuk P., Geiselhart S., Battin C., Webb T.J., Steinberger P.,
RA   Paster W., Hoffmann-Sommergruber K.;
RT   "Generation of a Jurkat-based fluorescent reporter cell line to
RT   evaluate lipid antigen interaction with the human iNKT cell
RT   receptor.";
RL   Sci. Rep. 9:7426.1-7426.12(2019).
//