ID   HGELN
AC   CVCL_WI90
SY   HeLa GAL4RE-ERE Luciferase-Neo
DR   Wikidata; Q94204125
RX   PubMed=10492897;
CC   Population: African American.
CC   Biotechnology: Used in a bioluminescent bioassay to quantitatively measure estrogenic compounds.
CC   Characteristics: The luciferase reporter gene is under the control of an estrogen-responsive element from the beta-globin promoter.
CC   Characteristics: Transfected with the DNA-binding domain (DBD) of GAL4 fused to the ligand-binding domain (LBD) of ESR1 (GAL4-ER).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:3467; ESR1 (Note=Ligand-binding domain).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P04386; Yeast GAL4 (Note=With p.Ile148_Glu881del = DNA-binding domain).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0030 ! HeLa
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 24-05-19; Last updated: 10-04-25; Version: 10
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RX   PubMed=10492897; DOI=10.1016/S0048-9697(99)00178-3;
RA   Balaguer P., Francois F., Comunale F., Fenet H., Boussioux A.-M.,
RA   Pons M., Nicolas J.-C., Casellas C.;
RT   "Reporter cell lines to study the estrogenic effects of
RT   xenoestrogens.";
RL   Sci. Total Environ. 233:47-56(1999).
//