ID   N2a-PK1
AC   CVCL_WI10
SY   N2aPK1; PK1
DR   Wikidata; Q95991905
RX   PubMed=14504404;
RX   PubMed=18077360;
CC   Biotechnology: Used in the scrapie cell (SC) assay. This susceptible cell line is exposed to mouse prion-containing samples for 3 days, grown to confluence and split 1:10 three times. The proportion of PrPSc-containing cells is determined with automated counting equipment.
CC   Characteristics: Highly susceptible to infection by prion (PrPsc).
CC   Derived from site: In situ; Peripheral nervous system; UBERON=UBERON_0000010.
CC   Cell type: Neural crest derived neuroblast; CL=CL_0002676.
CC   Breed/subspecies: A/J.
DI   NCIt; C21993; Mouse neuroblastoma
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_WI09 ! N2a-PD88
SX   Male
CA   Cancer cell line
DT   Created: 24-05-19; Last updated: 29-06-23; Version: 5
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RX   PubMed=14504404; DOI=10.1073/pnas.1834432100; PMCID=PMC208815;
RA   Klohn P.-C., Stoltze L., Flechsig E., Enari M., Weissmann C.;
RT   "A quantitative, highly sensitive cell-based infectivity assay for
RT   mouse scrapie prions.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:11666-11671(2003).
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RX   PubMed=18077360; DOI=10.1073/pnas.0710054104; PMCID=PMC2409240;
RA   Mahal S.P., Baker C.A., Demczyk C.A., Smith E.W., Julius C.,
RA   Weissmann C.;
RT   "Prion strain discrimination in cell culture: the cell panel assay.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:20908-20913(2007).
//