ID   E-PR293
AC   CVCL_VV75
DR   Wikidata; Q93533965
RX   PubMed=16815068;
CC   Characteristics: Can be used to screen HIV-1 protease inhibitors. Produces HIV-1 PR fused to GFP in the presence of doxycycline.
CC   Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P12497; HIV-1 isolate NY5 pol.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_V349 ! HEK 293 Tet-Off
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 24-05-19; Last updated: 10-04-25; Version: 9
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RX   PubMed=16815068; DOI=10.1016/j.micinf.2006.02.016;
RA   Fuse T., Watanabe K., Kitazato K., Kobayashi N.;
RT   "Establishment of a new cell line inducibly expressing HIV-1 protease
RT   for performing safe and highly sensitive screening of HIV protease
RT   inhibitors.";
RL   Microbes Infect. 8:1783-1789(2006).
//