ID   HeLa EGFP-654
AC   CVCL_VS42
DR   Wikidata; Q94096364
WW   http://technologies.unc.edu/technologies/16-0176_cell-line-hela-egfp-654-available-with-egfp-or-luciferase-pluc-705
CC   Population: African American.
CC   Characteristics: Transfected with a construct containing a mutated human beta-globin intron, IVS2-654, at nucleotide 105 of EGFP cDNA thus preventing its correct translation. Treatment of the cells expressing the construct with active antisense oligonucleotide should restore proper splicing and translation of EGFP, providing a rapid and sensitive positive readout for antisense activity in the nuclei of the treated cells.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0058 ! HeLa S3
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 25-02-19; Last updated: 05-10-23; Version: 6
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