ID   HeLa EGFP-654
AC   CVCL_VS42
DR   Wikidata; Q94096364
RX   PubMed=11574678;
RX   PubMed=11748845;
WW   Provider; UNC OTC; 16-0176; https://unc.flintbox.com/technologies/2512ea18-bd35-4199-8769-0c6bd96cbcdc
CC   Population: African American.
CC   Characteristics: Transfected with a construct containing a mutated human beta-globin intron, HBB IVS2-654, at nucleotide 105 of EGFP cDNA thus preventing its correct translation. Treatment of the cells expressing the construct with active antisense oligonucleotide should restore proper splicing and translation of EGFP, providing a rapid and sensitive positive readout for antisense activity in the nuclei of the treated cells (PubMed=11574678; PubMed=11748845).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0058 ! HeLa S3
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 25-02-19; Last updated: 10-04-25; Version: 8
//
RX   PubMed=11574678; DOI=10.1093/nar/29.19.3965; PMCID=PMC60237;
RA   Sazani P., Kang S.-H., Maier M.A., Wei C.-F., Dillman J., Summerton J.,
RA   Manoharan M., Kole R.;
RT   "Nuclear antisense effects of neutral, anionic and cationic
RT   oligonucleotide analogs.";
RL   Nucleic Acids Res. 29:3965-3974(2001).
//
RX   PubMed=11748845; DOI=10.1002/humu.1229;
RA   Gemignani F., Landi S., DeMarini D.M., Kole R.;
RT   "Spontaneous and MNNG-induced reversion of an EGFP construct in HeLa
RT   cells: an assay for observing mutations in living cells by
RT   fluorescent microscopy.";
RL   Hum. Mutat. 18:526-534(2001).
//