ID   WISC014i-SC1
AC   CVCL_VS05
SY   GFP-CCR5mut-hiPSC-Comb2-SC1
DR   Wikidata; Q98134936
RX   PubMed=26670276;
CC   From: University of Wisconsin; Madison; USA.
CC   Characteristics: The expression of the Clover GFP gene is under the control of the human EF1a promoter.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:1606; CCR5.
CC   Genetic integration: Method=Transfection; Gene=FPbase; 4Z641; Clover (Note=Basic green/yellow fluorescent protein derived from GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
CC   Cell type: Fibroblast of foreskin; CL=CL_1001608.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_VS02 ! WISC011i-inGFPpuro
SX   Male
AG   <1M
CA   Induced pluripotent stem cell
DT   Created: 25-02-19; Last updated: 19-12-24; Version: 11
//
RX   PubMed=26670276; DOI=10.1038/mtna.2015.42;
RA   Kang H., Minder P., Park M.A., Mesquitta W.-T., Torbett B.E.,
RA   Slukvin I.I.;
RT   "CCR5 disruption in induced pluripotent stem cells using CRISPR/Cas9
RT   provides selective resistance of immune cells to CCR5-tropic HIV-1
RT   virus.";
RL   Mol. Ther. Nucleic Acids 4:e268.1-e268.10(2015).
//