ID   HEK293-SREBP1-T2A-luciferase-KI
AC   CVCL_VR42
DR   Wikidata; Q94095267
RX   PubMed=30232659;
CC   Characteristics: Useful for studying transcriptional regulation of the SREBP1 gene and for screening drugs or chemical molecules that regulate SREBP1 gene expression (PubMed=30232659).
CC   Characteristics: CRISPR/Cas9-mediated knock-in of a T2A-luciferase-CMV-eGFP-T2A-Neomycin construct at the C-terminal end of SREBP1.
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0045 ! HEK293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 13-11-18; Last updated: 10-04-25; Version: 7
//
RX   PubMed=30232659; DOI=10.1007/s10529-018-2608-2;
RA   Li Z.-H., Zhao J.-L., Muhammad N., Wang D.-Y., Mao Q.-W., Xia H.-B.;
RT   "Establishment of a HEK293 cell line by CRISPR/Cas9-mediated
RT   luciferase knock-in to study transcriptional regulation of the human
RT   SREBP1 gene.";
RL   Biotechnol. Lett. 40:1495-1506(2018).
//